Cryopreservation of mango (Mangifera indica L.) embryogenic cultures

文献类型： 外文期刊

第一作者： Wu, YJ

作者： Wu, YJ;Huang, XL;Xiao, JN;Li, XJ;Zhou, MD;Engelmann, F

作者机构：

关键词： Mangifera indica;embryogenic masses;vitrification;encapsulation/dehydration;pregrowth/dehydration

期刊名称：CRYOLETTERS （ 影响因子：1.066； 五年影响因子：1.0 ）

ISSN： 0143-2044

年卷期： 2003 年 24 卷 5 期

页码：

收录情况： SCI

摘要： Three techniques were compared for cryopreserving embryogenic masses (EMs) sampled from mango (Mangifera indica L.) cv. Zihua embryogenic cultures: (i) encapsulation/dehydration; (ii) pregrowth/dehydration; and (iii) vitrification. In all experiments, EMs were sampled from embryogenic cultures during their exponential growth phase and pretreated for 24 h on solid medium containing 0.5 M sucrose before freezing. No recovery was achieved after cryopreservation using the encapsulation/dehydration technique, whatever the moisture content (fresh weight basis) of EMs, which ranged from 78.3 % without dehydration to 40.8 % after 6 h dehydration. With the pregrowth/dehydration technique, limited recovery (8.3 %) was achieved after desiccation of EMs for I h, to 58.5 % MC. Using the vitrification technique, recovery ranged from 94.3 % after treatment of EMs with the PVS3 vitrification solution for 20 min (EM moisture content of 34.7 %) to 10.9 % after a 120 min treatment with the vitrification solution (EM moisture content of 26.0 %).

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