Chloroplast transformation in oilseed rape

文献类型： 外文期刊

第一作者： Hou, BK

作者： Hou, BK;Zhou, YH;Wan, LH;Zhang, ZL;Shen, GF;Chen, ZH;Hu, ZM

作者机构：

关键词： chloroplast transformation;oilseed rape;site-specific integration

期刊名称：TRANSGENIC RESEARCH （ 影响因子：2.788； 五年影响因子：2.377 ）

ISSN： 0962-8819

年卷期： 2003 年 12 卷 1 期

页码：

收录情况： SCI

摘要： The chloroplast transformation vector pNRAB carries two expression cassettes for the spectinomycin resistance gene aadA and the insect resistance gene cry1Aa10. The two cassettes are sited between the rps7 and ndhB targeting fragments. Biolistic delivery of the vector DNA, followed by spectinomycin selection, yielded chloroplast transformants at a frequency of four in 1000 bombarded cotyledon petioles. PCR analysis and Southern blot of PCR products confirmed the site-specific integration of aadA and cry1Aa10 into the chloroplast genomes of transgenic oilseed rape. When transgenic oilseed rape leaves were fed to second instar Plutella xylostera larvae, 47% mortality was observed against this insect and the surviving larvae had significantly lower weight than the control. This is the first report of chloroplast transformation in oilseed rape and the introduction of novel genes between the rps7 and ndhB genes in the chloroplast genome. This offers an opportunity for improvement of oilseed rape by chloroplast genetic engineering.

分类号：

  • 相关文献

[1]Co-Transfer of TALENs Construct Targeted for Chloroplast Genome and Chloroplast Transformation Vector Into Rice Using Particle Bombardment. Li, Ding,Tang, Ning,Fang, Zhen,Xia, Yumei,Cao, Mengliang,Li, Ding,Tang, Ning.

[2]Testing the Role of the N-Terminal Tail of D1 in the Maintenance of Photosystem II in Tobacco Chloroplasts. Michoux, Franck,Ahmad, Niaz,Wei, Zheng-Yi,Nixon, Peter J.,Belgio, Erica,Ruban, Alexander V.,Michoux, Franck,Ahmad, Niaz,Wei, Zheng-Yi,Wei, Zheng-Yi,Belgio, Erica. 2016

[3]Transformation of alfalfa chloroplasts and expression of green fluorescent protein in a forage crop. Wei, Zhengyi,Liu, Yanzhi,Lin, Chunjing,Wang, Yunpeng,Cai, Qin'an,Dong, Yingshan,Xing, Shaochen. 2011

[4]The Klebsiella pneumoniae nitrogenase Fe protein gene (nifH) functionally substitutes for the chlL gene in Chlamydomonas reinhardtii. Cheng, Q,Day, A,Dowson-Day, M,Shen, GF,Dixon, R. 2005

[5]Establishment of a Gene Expression System in Rice Chloroplast and Obtainment of PPT-Resistant Rice Plants. Li Yi-nue,Zhang Zhi-fang,Shen Gui-fang,Sun Bing-yao,Meng Xiang-xun,Su Ning. 2009

[6]Studies on insect resistance of Bt transplastomic plants and the phenotype of their progenies. Zhang, ZL,Chen, X,Qian, KX,Shen, GF. 1999

[7]Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast. Sun, M,Qian, KX,Su, N,Chang, HY,Liu, JX,Chen, GF.

[8]Quantitative trait loci mapping for two seed yield component traits in an oilseed rape (Brassica napus) cross. WEI CHEN,YONGSHAN ZHANG,JINBO YAO,CHAOZHI MA,JINXING TU,FU TINGDONG. 2011

[9]Development of a biologically based fertilizer, incorporating Bacillus megaterium A6, for improved phosphorus nutrition of oilseed rape. Hu, Xiaojia,Xie, Lihua,Yu, Changbing,Li, Yinshui,Zhang, Shujie,Liao, Xing,Roberts, Daniel P.,Maul, Jude E..

[10]New host resistances to Pseudocercosporella capsellae and implications for white leaf spot management in Brassicaceae crops. Gunasinghe, Niroshini,You, Ming Pei,Barbetti, Martin J.,Barbetti, Martin J.,Li, Xi Xiang,Banga, Surinder S.,Banga, Shashi K..

[11]Efficient Detection of Leptosphaeria maculans from Infected Seed lots of Oilseed Rape. Song, Pei-ling,Bao, Yu-ying,Song, Pei-ling,Jedryczka, Malgorzata,Irzykowski, Witold,Song, Pei-ling,Yan, Meng-jiao,Huangfu, Hai-yan,Hao, Li-fen,Li, Zi-qin.

[12]A robust sampling approach for identification and quantification of methyl jasmonate in leaf tissue of oilseed rape for analysis of early signaling in sclerotinia sclerotiorum resistance. Wei, Fang,Cheng, Ji-hua,Liu, Sheng-yi,Huang, Jun-yan,Dong, Xu-yan,Li, Ping-ping,Kong, Fan-pi,Wu, Yu,Li, Yan-hua,Chen, Hong,Wang, Zhan,Feng, Yu-qi.

[13]A mutant of the nematophagous fungus Paecilomyces lilacinus (Thom) is a novel biocontrol agent for Sclerotinia sclerotiorum. Yang, Fan,Abdelnabby, Hazem,Xiao, Yannong,Yang, Fan,Abdelnabby, Hazem.

[14]Effects of imidacloprid and thiamethoxam as seed treatments on the early seedling characteristics and aphid-resistance of oilseed rape. Huang Liang,Lu Yao-bin,Huang Liang,Zhao Chun-lin,Huang Fang,Lu Yao-bin,Bai Run-e,Yan Feng-ming,Hao Zhong-ping. 2015

[15]Rhizosphere cadmium speciation and mechanisms of cadmium tolerance in different oilseed rape species. Ru, SH,Xing, JP,Su, DC. 2006

[16]Influence of subsoil zinc on dry matter production, seed yield and distribution of zinc in oilseed rape genotypes differing in zinc efficiency. Grewal, HS,Lu, ZG,Graham, RD. 1997

[17]BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape. Chen, Quan,Wang, Jie,Hou, Mingsheng,Cai, Li,Chen, Quan,Liu, Shengyi,Huang, Junyan. 2015

[18]Formulations of Bacillus subtilis BY-2 suppress Sclerotinia sclerotiorum on oilseed rape in the field. Hu, Xiaojia,Xie, Lihua,Yu, Changbing,Li, Yinshui,Jiang, Mulan,Liao, Xiangsheng,Che, Zhi,Liao, Xing,Roberts, Daniel P.,Maul, Jude E.. 2014

[19]Formulations of the endophytic bacterium Bacillus subtilis Tu-100 suppress Sclerotinia sclerotiorum on oilseed rape and improve plant vigor in field trials conducted at separate locations. Roberts, Daniel P.,Maul, Jude E.,Emche, Sarah E.,McKenna, Laurie F.,Buyer, Jeffrey S.,Hu, Xiaojia,Liao, Xing,Guo, Xuelan,Liu, Yeying,Liu, Shengyi. 2011

[20]Expression divergence of FRUITFULL homeologs enhanced pod shatter resistance in Brassica napus. Peng, P. F.,Li, Y. C.,Mei, D. S.,Fu, L.,Liu, J.,Chen, Y. F.,Hu, Q.,Colasanti, J.. 2015

作者其他论文 更多>>

微信小程序