Expressing PHB synthetic genes through chloroplast genetic engineering

文献类型: 外文期刊

第一作者: Zhang, JY

作者: Zhang, JY;Su, N;Zhang, ZL;Zhao, HY;Zhu, SW;Song, YR

作者机构:

关键词: chloroplast genetic engineering;poly-3-hydroxybutyrate;transplastomic tobacco;transcriptional level;gene silencing

期刊名称:CHINESE SCIENCE BULLETIN ( 影响因子:1.649; 五年影响因子:1.738 )

ISSN: 1001-6538

年卷期: 2002 年 47 卷 16 期

页码:

收录情况: SCI

摘要: Chloroplast integration and expression vector containing expression cassettes for phbB, phbA, phbC and aadA genes was constructed and bombarded into the tobacco chloroplast genome. Transplastomic plants were analyzed with PCR and Southern blot. Their homoplastomy was also judged. Northern dot and RT-PCR analysis were employed to investigate transgene expression at transcriptional level. The results indicate that the chloroplast transformation system is compatible for poly-3-hydroxybutyrate (PHB) production.

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