Expressing PHB synthetic genes through chloroplast genetic engineering

文献类型: 外文期刊

第一作者: Zhang, JY

作者: Zhang, JY;Su, N;Zhang, ZL;Zhao, HY;Zhu, SW;Song, YR

作者机构: Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China;Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China; Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

关键词: chloroplast genetic engineering;poly-3-hydroxybutyrate;transplastomic tobacco;transcriptional level;gene silencing

期刊名称:CHINESE SCIENCE BULLETIN ( 2016影响因子:1.649; 五年影响因子:1.738 )

ISSN: 1001-6538

年卷期: 2002 年 47 卷 16 期

页码:

收录情况: SCI

摘要: Chloroplast integration and expression vector containing expression cassettes for phbB, phbA, phbC and aadA genes was constructed and bombarded into the tobacco chloroplast genome. Transplastomic plants were analyzed with PCR and Southern blot. Their homoplastomy was also judged. Northern dot and RT-PCR analysis were employed to investigate transgene expression at transcriptional level. The results indicate that the chloroplast transformation system is compatible for poly-3-hydroxybutyrate (PHB) production.

分类号:

  • 相关文献

[1]Synthesis of medium-chain-length-polyhydroxyalkanoates in tobacco via chloroplast genetic engineering. Wang, YH,Wu, ZY,Zhang, XH,Chen, GQ,Wu, Q,Huang, CL,Yang, Q.

[2]Cloning and Expression of Two Soluble Acid Invertase Gene Isoforms from Rhododendron. He Lisi,Su Jiale,Liu Xiaoqing,Li Chang,Chen Shangping. 2014

[3]Improved Production of Raw Starch Degrading Enzyme by Aspergillus oryzae F-30 Using Methyl Glucoside Sesqui-Stearate. Sun, Hai-Yan,Peng, Ming,Wang, Lu,Liu, Jian-Wen. 2009

[4]Silencing of dehydrin CaDHN1 diminishes tolerance to multiple abiotic stresses in Capsicum annuum L.. Chen, Ru-gang,Jing, Hua,Guo, Wei-li,Ma, Fang,Gong, Zhen-Hui,Guo, Wei-li,Wang, Shu-Bin,Pan, Bao-Gui,Gong, Zhen-Hui.

[5]A highly efficient method for construction of rice artificial MicroRNA vectors. Chen, Jianping.

[6]ARGONAUTE SUBFAMILY GENES IN THE SMALL BROWN PLANTHOPPER, Laodelphax striatellus (HEMIPTERA: DELPHACIDAE). Zhou, Yan-Ru,Zhou, Yan-Ru,Li, Jun-Min,Sun, Zong-Tao,Xie, Li,Chen, Jian-Ping,Li, Lin-Ying.

[7]Temperature-Dependent Survival of Turnip Crinkle Virus-Infected Arabidopsis Plants Relies on an RNA Silencing-Based Defense That Requires DCL2, AG02, and HEN1. Zhang, Xiuchun,Zhang, Xiaofeng,Singh, Jasleen,Qu, Feng,Zhang, Xiuchun,Zhang, Xiaofeng,Li, Dawei.

[8]Expressional and regulatory characterization of Arabidopsis RNA-dependent RNA polymerase 1. Xu, Tao,Zhang, Liang,Zhen, Jie,Fan, Yunliu,Zhang, Chunyi,Wang, Lei.

[9]Cloning and functional characterization of a cation-chloride cotransporter gene OsCCC1. Kong, Xiang-Qiang,Gao, Xiu-Hua,Sun, Wei,An, Jing,Zhao, Yan-Xiu,Zhang, Hui,Kong, Xiang-Qiang,An, Jing.

[10]Progress in the studies of foreign gene silencing in transgenic plants.. Zhu, L,Zhang, CY,Fan, YL. 1999

[11]Translation Initiation Factor eIF4E and eIFiso4E Are Both Required for Peanut stripe virus Infection in Peanut (Arachis hypogaea L.). Xu, Manlin,Xie, Hongfeng,Wu, Juxiang,Chi, Yucheng,Xu, Manlin,Xie, Lianhui,Yang, Jinguang. 2017

[12]Cloning and functional analysis of an endo-PG-encoding gene Rrspg1 of Rhizoctonia solani, the causal agent of rice sheath blight. Yang, Ying-Qing,Yang, Mei,Li, Ming-Hai,Zhou, Er-Xun,Yang, Ying-Qing. 2012

[13]Study on the RNA interference of Sry in mouse embryos. Zhao Jin-Hong,Du Wei-Hua,Dao Er-Ji,Pei Jie,Zhang Li,Lin Xiu-Kun. 2006

[14]Influence of cellulase gene knockdown by dsRNA interference on the development and reproduction of the pine wood nematode, Bursaphelenchus xylophilus. Dai, Su-Ming,Xiao, Luo,Xie, Bing-Yan,Cheng, Xin-Yue. 2010

[15]Construction of a genomewide RNAi mutant library in rice. Wang, Lei,Zheng, Jie,Luo, Yanzhong,Xu, Tao,Zhang, Qiuxue,Zhang, Lan,Xu, Miaoyun,Zhang, Chunyi,Fan, Yunliu,Wan, Jianmin,Wang, Ming-Bo. 2013

[16]Phytophthora effector targets a novel component of small RNA pathway in plants to promote infection. Qiao, Yongli,Shi, Jinxia,Zhai, Yi,Hou, Yingnan,Ma, Wenbo,Qiao, Yongli,Shi, Jinxia,Hou, Yingnan,Ma, Wenbo,Qiao, Yongli.

[17]Generation of transgenic wheat lines with altered expression levels of 1Dx5 high-molecular weight glutenin subunit by RNA interference. Yue, S. J.,Li, Y. W.,Zhu, Y. F.,Chen, Y.,Jia, X.,Li, H.,Yue, S. J.. 2008

[18]Characterization and subcellular localization of an RNA silencing suppressor encoded by Rice stripe tenuivirus. Xiong, Ruyi,Wu, Jianxiang,Zhou, Xueping,Zhou, Yijun. 2009

[19]Versatile glycoside hydrolase family 18 chitinases for fungi ingestion and reproduction in the pinewood nematode Bursaphelenchus xylophilus. Wang, Xuan,Guan, Tinglong,Li, Hongmei,Peng, Deliang.

[20]Gene knockdown by intro-thoracic injection of double-stranded RNA in the brown planthopper, Nilaparvata lugens. Ding, Zhiping,Liu, Zewen,Liu, Shuhua,Yang, Baojun,Zhang, Chengwei.

作者其他论文 更多>>