Characterization of euploid backcross progenies derived from interspecific hybrids between Oryza sativa and O-eichingeri by restriction fragment length polymorphism (RFLP) analysis and genomic in situ hybridization (GISH)

文献类型： 外文期刊

第一作者： Yan, HH

作者： Yan, HH;Liu, GQ;Cheng, ZK;Min, SK;Zhu, LH

作者机构：

关键词： Oryza sativa;Oryza eichingeri;introgression lines;RFLP;genomic in situ hybridization (GISH)

期刊名称：GENOME （ 影响因子：2.166； 五年影响因子：2.474 ）

ISSN： 0831-2796

年卷期： 2001 年 44 卷 1 期

页码：

收录情况： SCI

摘要： Restriction fragment length polymorphism (RFLP) analysis and GISH (genomic in situ hybridization) were performed on euploid plants derived from crosses between Oryza sativa (2n = 24, AA) and two brown planthopper-resistant accessions of O. eichingeri (2n = 24, CC). After screening with 164 RFLP markers, 60 of the 67 euploid plants were identified as introgression lines, each carrying 1-6 small O. eichingeri segments integrated on chromosomes 1, 2, 6, or 10. In the somatic chromosome preparations of F-1 hybrid, O. eichingeri chromosomes, fluorescing greenish-yellow in the sequential GISH, appeared to be longer and to contain more heterochromatin than O. sativa ones, and this karyotypic polymorphism can be used to detect some introgressed O. eichingeri segments in euploid plants. In addition, GISH identification presented direct evidence for the transfer of small segments from O. eichingeri to O. sativa chromosome(s) which were subsequently recognized according to their condensation pattern, arm ratio, and chromosome length. The present results would contribute to the molecular mapping and selection of O. eichingeri - derived brown planthopper-resistant gene and positive yield QTLs.

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