Characterization of euploid backcross progenies derived from interspecific hybrids between Oryza sativa and O-eichingeri by restriction fragment length polymorphism (RFLP) analysis and genomic in situ hybridization (GISH)

文献类型： 外文期刊

第一作者： Yan, HH

作者： Yan, HH;Liu, GQ;Cheng, ZK;Min, SK;Zhu, LH

作者机构：

关键词： Oryza sativa;Oryza eichingeri;introgression lines;RFLP;genomic in situ hybridization (GISH)

期刊名称：GENOME （ 影响因子：2.166； 五年影响因子：2.474 ）

ISSN： 0831-2796

年卷期： 2001 年 44 卷 1 期

页码：

收录情况： SCI

摘要： Restriction fragment length polymorphism (RFLP) analysis and GISH (genomic in situ hybridization) were performed on euploid plants derived from crosses between Oryza sativa (2n = 24, AA) and two brown planthopper-resistant accessions of O. eichingeri (2n = 24, CC). After screening with 164 RFLP markers, 60 of the 67 euploid plants were identified as introgression lines, each carrying 1-6 small O. eichingeri segments integrated on chromosomes 1, 2, 6, or 10. In the somatic chromosome preparations of F-1 hybrid, O. eichingeri chromosomes, fluorescing greenish-yellow in the sequential GISH, appeared to be longer and to contain more heterochromatin than O. sativa ones, and this karyotypic polymorphism can be used to detect some introgressed O. eichingeri segments in euploid plants. In addition, GISH identification presented direct evidence for the transfer of small segments from O. eichingeri to O. sativa chromosome(s) which were subsequently recognized according to their condensation pattern, arm ratio, and chromosome length. The present results would contribute to the molecular mapping and selection of O. eichingeri - derived brown planthopper-resistant gene and positive yield QTLs.

分类号：

  • 相关文献

[1]A genome-specific repetitive DNA sequence from Oryza eichingeri: characterization, localization, and introgression to O-sativa. Yan, HH,Liu, GQ,Cheng, ZK,Li, XB,Liu, GZ,Min, SK,Zhu, LH. 2002

[2]Molecular characterization of a Thinopyrum intermedium Group 2 chromosome (2Ai-2) conferring resistance to barley yellow dwarf virus. Zhang, ZY,Xin, ZY,Larkin, PJ. 2001

[3]Identification of molecular markers for the Thinopyrum intermedium chromosome 2Ai-2 with resistance to barley yellow dwarf virus. Zhang, ZY,Xin, ZY,Lin, ZS,Chen, X,Wang, XP. 2000

[4]Identification of Wheat-Barley 2H Alien Substitution Lines. Yuan, YP,Chen, X,Xiao, SH,Islam, AKMR,Xin, ZY.

[5]Visualization of Oryza eichingieri chromosomes in intergenomic hybrid plants from O-sativa x O-eichingeri via fluorescent in situ hybridization. Yan, HH,Min, SK,Zhu, LH. 1999

[6]Relationship between molecular marker heterozygosity and hybrid performance in intra- and inter-subspecific crosses of rice. Zhao, MF,Li, XH,Yang, JB,Xu, CG,Hu, RY,Liu, DJ,Zhang, Q. 1999

[7]Mapping of a new gene for brown planthopper resistance in cultivated rice introgressed from Oryza eichingeri. Liu, GQ,Yan, HH,Fu, Q,Qian, Q,Zhang, ZT,Zhai, WX,Zhu, LH.

[8]Dissection of heterosis for yield and related traits using populations derived from introgression lines in rice. Xiang, Chao,Zhang, Hongjun,Wei, Shaobo,Fu, Binying,Gao, Yongming,Wang, Hui,Xia, Jiafa,Li, Zefu,Ye, Guoyou. 2016

[9]Mining for Candidate Genes in an Introgression Line by Using RNA Sequencing: The Anthocyanin Overaccumulation Phenotype in Brassica. Xie, Lulu,Li, Fei,Zhang, Shifan,Zhang, Hui,Qian, Wei,Li, Peirong,Zhang, Shujiang,Sun, Rifei. 2016

[10]Scanning QTLs for Grain Shape using Two Sets of Introgression Lines in Rice. Qiu, Xianjin,Du, Bin,Hu, Hui,Ou, Xiaoxue,Lv, Wenkai,Yang, Longwei,Xing, Danying,Xu, Junying,Li, Zhixin,Zhang, Yunbo,Wang, Xiaoyan,Xu, Jianlong,Xu, Jianlong,Zheng, Tianqing,Qiu, Xianjin. 2017

[11]Genetic and Epigenetic Alterations of Brassica nigra Introgression Lines from Somatic Hybridization: A Resource for Cauliflower Improvement. Wang, Gui-xiang,Lv, Jing,Zhang, Jie,Han, Shuo,Zong, Mei,Guo, Ning,Zeng, Xing-ying,Zhang, Yue-yun,Liu, Fan,Lv, Jing,Wang, You-ping,Lv, Jing. 2016

[12]Dissection of combining ability for yield and related traits using introgression lines in the background of a key restorer line in rice (Oryza sativa L.). Xiang, Chao,Zhang, Hongjun,Wang, Jie,Wang, Wensheng,Gao, Yongming,Wang, Hui,Xia, Jiafa,Ye, Guoyou.

[13]Identification of QTLs for red fruit firmness using the wild tomato species Solanum pennellii LA716 introgression lines. Yang, Shengbao,Wang, Baike,Xu, Jian,Gao, Jie,Yang, Shengbao,Yu, Qinghui,Wang, Baike,Yang, Tao,Li, Ning,Tang, Yaping,Aisimutuola, Patiguli,Wang, Qiang.

[14]QTL detection of amino acid content in grains of rice using advanced backcross introgression lines. Cheng, Li-Rui,Luo, Cheng-Gang,Xu, Jian-Long. 2013

[15]Production and characterization of interspecific somatic hybrids between Brassica oleracea var. botrytis and B. nigra and their progenies for the selection of advanced pre-breeding materials. Wang, Gui-xiang,Tang, Yu,Sheng, Xiao-guang,Hao, Wei-wei,Zhang, Li,Lu, Kun,Liu, Fan,Yan, Hong. 2011

[16]Chromosomal distribution of the 18S-5.8S-26S rDNA loci and heterogeneity of nuclear in regions in Thinopyrum intermedium (Poaceae : Triticeae). Li, DY,Ru, YY,Zhang, XY. 2004

[17]Primary identification of alien chromatin in T911289, a maintainer of wheat male sterile line with cytoplasm of Aegilops kotschyi. Liu, BS,Li, DY,Zhang, XY,Gao, QR,Sun, LZ,Sun, QX,Dong, ST. 2003

[18]Chromosomal Distribution of the 18S-5.8S-26S rDNA Loci and Heterogeneity of Nuclear ITS Regions in Thinopyrum intermedium (Poaceae: Triticeae). Li Dayong,Ru Yanyan,Zhang Xueyong. 2004

[19]Molecular cytogenetic identification of a wheat-Thinopyron intermedium (Host) Barkworth & DR Dewey partial amphiploid resistant to powdery mildew. Wang, HG,Zhang, XY,Li, XF,Li, DY,Duan, XY,Zhou, YL. 2005

[20]Genetic relationship and genomic in situ hybridization analysis of the three genomes in Triticum aestivum. Li, DY,Zhang, XY,Yang, J,Rao, GY. 2000

作者其他论文 更多>>

微信小程序