Visualization of Oryza eichingieri chromosomes in intergenomic hybrid plants from O-sativa x O-eichingeri via fluorescent in situ hybridization

文献类型: 外文期刊

第一作者: Yan, HH

作者: Yan, HH;Min, SK;Zhu, LH

作者机构:

关键词: fluorescent in situ hybridization;Oryza sativa;Oryza eichingeri;amphiploid;alien addition lines

期刊名称:GENOME ( 影响因子:2.166; 五年影响因子:2.474 )

ISSN: 0831-2796

年卷期: 1999 年 42 卷 1 期

页码:

收录情况: SCI

摘要: Digoxigenin-labeled total genomic DNA from Oryza eichingeri was hybridized in situ to somatic chromosome preparations of F-1, F-2, backcross progenies, and a pollen-derived tetraploid plant (E-24) from O. sativa (2n = 24, genome AA) x O. eichingeri (2n = 24, genome CC), which allowed a definitive discrimination between A- and C-genome chromosomes. Twelve chromosomes in F-1, F-2, BC1, and twenty-four chromosomes in plant E-24 were clearly revealed to be of O. eichingeri origin, thus confirming that both BC1 and F-2 were allotriploids (2n = 36, AAC) while plant E-24 was an amphiploid (2n = 48, AACC). In addition, the presence of O. eichingeri chromosomes in four alien addition lines was characterized. The results suggest that FISH/GISH may be a powerful tool for monitoring the O. sativa-alien chromosome in the progenies of rice interspecific hybridization.

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