Development of pepper SSR markers from sequence databases

文献类型： 外文期刊

第一作者： Huang, SW

作者： Huang, SW;Zhang, BX;Milbourne, D;Cardle, L;Yang, GM;Guo, JZ

作者机构：

关键词： Capsicum annuum L.;database search;simple sequence repeats;SSR polymorphism

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2001 年 117 卷 2 期

页码：

收录情况： SCI

摘要： Simple sequence repeat (SSR) markers are potentially valuable tools for plant breeding. The objectives of the work reported here were to search the EMBL and Genbank databases for the presence of SSR-containing sequences from the genus Capsicum, to assess the frequency of different motifs, and to examine the polymorphism of selected markers in a panel of genotypes, including 10 Capsicum spp. and 1 tomato and 1 potato genotype. Fifty-eight microsatellites with different motifs were found in Capsicum sequences. A subset of twelve of these were selected for the polymorphism survey using PCR primers flanking the SSR. Polymorphisms between Capsicum lines can be detected with 5 of these primer pairs. PCR products of the predicted size were also amplified with three primer pairs in potato and one primer pair in tomato. The study also showed that shorter microsatellites could be valuable markers in Capsicum.

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