Two RAPD markers linked to a major fertility restorer gene in pepper

文献类型： 外文期刊

第一作者： Zhang, BX

作者： Zhang, BX;Huang, SW;Yang, GM;Guo, JZ

作者机构：

关键词： bulked segregant analysis;fertility restorer gene;marker assisted selection (MAS);pepper (Capsicum annuum L.);random amplified polymorphic DNA (RAPD)

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2000 年 113 卷 2 期

页码：

收录情况： SCI

摘要： Both major and minor genes control the restoring of fertility in the cytoplasmic male-sterility system in pepper (Capsicum annuum L.). Bulked segregant analysis (BSA) was applied to identify molecular markers linked to a major restorer gene (Rf) using the F-2 population of Niujiaojiao No.21 (rfrf)/Xiangtanwan (RfRf). Two random amplified polymorphic DNA (RAPD) markers linked to this allele were detected with 520 decamer primers with arbitrary sequences. OP13(1400) is a tightly linked marker with a genetic distance of 0.37 cm. OW19(800) is on the opposite side with a distance of 8.12 cm. Both markers were repeatable and easy to score. A panel of genotypes, including 13 elite inbred lines with different fertility restoring ability, were assayed for the presence of OP13(1400) and OW19(800). The markers are absent in all sweet pepper lines, indicating that they will be most helpful for transferring Rf into sweet pepper lines. With the aid of these markers, the size of the backcross population for testcrosses can be minimized. Furthermore, these markers will be useful in genetic analysis of the minor genes.

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