DNA immunization elicits high HI antibody and protects chicken from AIV challenge

文献类型: 外文期刊

第一作者: Chen, H

作者: Chen, H;Yu, K;Jiang, Y;Tang, X

作者机构:

关键词: avian influenza virus;DNA immunization;H5N1 subtype

期刊名称:OPTIONS FOR THE CONTROL OF INFLUENZA IV

ISSN: 0531-5131

年卷期: 2001 年 1219 卷

页码:

收录情况: SCI

摘要: Background: A highly pathogenic avian influenza virus of goose origin A/Goose/Guangdong/1/ 96 (H5N1) (GD/96) shared nucleotide homology of 98% in the HA with A/HK/156/97(H5N1). The nucleotide sequences at the cleavage site, all potential glycosylation sites and the receptor binding sites in the HA were conserved. The purpose of this study was to evaluate the immunogenicity and efficacy of a DNA vaccine to against this virus. Methods: The HA cDNA from GD/96 was cloned under a CMV promoter to generate vaccine plasmid pCIHA, which was administered to 3-week-old SPF chickens in two doses of 100 mug (n=9) or 50 mug (n=14) by the intramuscular route, 2 weeks apart. Chickens were challenged with 10 LD50 GD/96 at 2 weeks post-boost. Results: Hemagglutinin inhibition (HI) antibody titers were 2(5.8+/-2.0) and 2(4.7+/-2.5), 2 weeks after priming, 2(8.4+/-2.7) and 2(7.6+/-3.9) and 2 weeks after boost, and 2(9.6+/-2.2) and 2(9.0+/-2.4), 1 week after challenge in chicken immunized with 100- or 50-mug pCIHA, respectively. All immunized chickens were fully protected from the lethal challenge, with no clinical signs of disease, no virus shedding and 100% survival at 10 days postchallenge. Conclusion: The plasinid pCIHA is highly immunogenic and protects SPF chickens from lethal challenge with the homologous avian influenza virus GD/96. (C) 2001 Elsevier Science B.V. All rights reserved.

分类号:

  • 相关文献

[1]Protective Efficacy of the Inactivated H5N1 Influenza Vaccine Re-6 Against Different Clades of H5N1 Viruses Isolated in China and the Democratic People's Republic of Korea. Zeng, Xianying,Deng, Guohua,Liu, Liling,Li, Yanbing,Shi, Jianzhong,Chen, Pucheng,Feng, Huapeng,Tian, Guobin,Chen, Hualan,Zeng, Xianying,Deng, Guohua,Liu, Liling,Li, Yanbing,Shi, Jianzhong,Chen, Pucheng,Feng, Huapeng,Tian, Guobin,Chen, Hualan,Liu, Jingli,Guo, Xingfu,Mao, Shenggang,Yang, Fan,Chen, Zhiyu.

[2]Protective Efficacy of an H5N1 Inactivated Vaccine Against Challenge with Lethal H5N1, H5N2, H5N6, and H5N8 Influenza Viruses in Chickens. Tian, Guobin,Chen, Hualan.

[3]Efficacy of DNA Immunization with F and G Protein Genes of Nipah Virus. Wang, Xijun,Ge, Jinying,Hu, Sen,Wang, Qinghua,Wen, Zhiyun,Chen, Hualan,Bu, Zhigao.

[4]Evaluation of the enhancing ability of three adjuvants for DNA vaccination using the porcine circovirus type 2 ORF2 (capsid) gene in mice. Fu, Fang,Lang, Yuekun,Li, Xuesong,Chai, Zheng,Liu, Siguo,Tian, Huabin,Li, Haizhong,Wang, Wei,Chen, Xin,Li, Xi,Li, Xuesong,Tong, Guangzhi.

[5]Co-expression of Ubiquitin gene and capsid protein gene enhances the potency of DNA immunization of PCV2 in mice. Fu, Fang,Li, Xuesong,Lang, Yuekun,Li, Xi,Li, Xuesong,Yang, Yuju,Tong, Guangzhi,Li, Guoxin,Zhou, Yanjun,Li, Xi. 2011

[6]PB2-E627K and PA-T97I substitutions enhance polymerase activity and confer a virulent phenotype to an H6N1 avian influenza virus in mice. Cheng, Kaihui,Yu, Zhijun,Sun, Weiyang,Xin, Yue,Huang, Jing,Zhang, Kun,Li, Xue,Yang, Songtao,Wang, Tiecheng,Zheng, Xuexing,Wang, Hualei,Qian, Jun,Gao, Yuwei,Xia, Xianzhu,Cheng, Kaihui,Yu, Zhijun,Qin, Chuan,Xia, Xianzhu,Yu, Zhijun,Qin, Chuan,Xia, Xianzhu,Zhang, Qianyi,Chen, Hualan,Chai, Hongliang,Hua, Yuping.

[7]Multiple adaptive amino acid substitutions increase the virulence of a wild waterfowl-origin reassortant H5N8 avian influenza virus in mice. Yu, Zhijun,Sun, Weiyang,Zhang, Xinghai,Xia, Xianzhu,Gao, Yuwei,Xia, Xianzhu,Gao, Yuwei,Cheng, Kaihui,Xia, Xianzhu,Gao, Yuwei. 2018

[8]Multiple amino acid substitutions involved in the adaptation of H6N1 avian influenza virus in mice. Yu, Zhijun,Qin, Chuan,Xia, Xianzhu,Yu, Zhijun,Qin, Chuan,Xia, Xianzhu,Yu, Zhijun,Cheng, Kaihui,Xin, Yue,Sun, Weiyang,Li, Xue,Huang, Jing,Zhang, Kun,Yang, Songtao,Wang, Tiecheng,Zheng, Xuexing,Wang, Hualei,Qin, Chuan,Xia, Xianzhu,Cheng, Kaihui,Chai, Hongliang.

[9]Rapid acquisition adaptive amino acid substitutions involved in the virulence enhancement of an H1N2 avian influenza virus in mice. Yu, Zhijun,Sun, Weiyang,Zhang, Xinghai,Xia, Xianzhu,Gao, Yuwei,Xia, Xianzhu,Gao, Yuwei,Cheng, Kaihui,Zhao, Chuqi.

[10]Sequence analysis of the haemagglutinin genes of H9N2 avian influenza viruses isolated in China during 1996 to 2011. Dai, Yin,Zhao, Ruihong,Hu, Xiaomiao,Zhang, Danjun,Wang, Junjun,Xu, Fazhi. 2012

[11]Diversified reassortant H9N2 avian influenza viruses in chicken flocks in northern and eastern China. Huang, Yanyan,Hu, Beixia,Zhang, Xiumei,Huang, Yanyan,Wen, Xintian,Cao, Sanjie,Du, Qijing,Gavrilov, Boris K.,Khan, Mazhar I.. 2010

[12]New H6 influenza virus reassortment strains isolated from Anser fabalis in Anhui Province, China. Ge, Ye,Yao, Qiucheng,Ma, Jian,Chen, Si,Deng, Guohua,Chen, Hualan,Ge, Ye,Chai, Hongliang,Hua, Yuping,Fan, Zhiqiang,Wang, Xianfu. 2017

[13]Novel genetic reassortants in H9N2 influenza A viruses and their diverse pathogenicity to mice. Bi, Yuhai,Lu, Lu,Li, Jing,Zeshan, Basit,Sun, Lei,Liu, Wenjun,Lu, Lu,Yin, Yanbo,Qin, Zhuoming,Zhang, Yi,Gao, Huijie,Liu, Jinhua. 2011

[14]Sequence and phylogenetic analysis of hemagglutinin genes of H9N2 influenza viruses isolated from chicken in China from 2013 to 2015. Su Xiao-na,Xie Qing-mei,Chen Wei-guo,Bi Ying-zuo,Chen Feng,Su Xiao-na,Xie Qing-mei,Chen Feng,Su Xiao-na,Xie Qing-mei,Chen Feng,Liao Chang-tao,Yan Zhuan-qiang. 2016

[15]The first lack of evidence of H7N9 avian influenza virus infections among pigs in Eastern China. Zhao, Fu-Rong,Zhou, Dong-Hui,Lin, Tong,Shao, Jun-Jun,Wei, Ping,Zhang, Yong-Guang,Chang, Hui-Yun,Wei, Ping.

[16]New influenza A(H7N7) viruses detected in live poultry markets in China. Cui, Pengfei,Chen, Hualan,Cui, Pengfei,Deng, Guohua,Shi, Jianzhong,Kong, Huihui,Liu, Liling,Guan, Yuntao,Chen, Hualan,Suzuki, Yasuo.

[17]Detection and differentiation of four poultry diseases using asymmetric reverse transcription polymerase chain reaction in combination with oligonucleotide microarrays. Tao, Qimeng,Wang, Xiurong,Bao, Hongmei,Wu, Jianan,Shi, Lin,Li, Yanbing,Qiao, Chuanling,Chen, Hualan,Tao, Qimeng,Wang, Xiurong,Bao, Hongmei,Wu, Jianan,Shi, Lin,Li, Yanbing,Qiao, Chuanling,Chen, Hualan,Yakovlevich, Samuilenko Anatolij,Mikhaylovna, Poukhova Nina.

[18]Efficacy of a high-yield attenuated vaccine strain wholly derived from avian influenza viruses by use of reverse genetics. Liu, Ming,Liu, Ming.

[19]Non-thermal plasma for inactivated-vaccine preparation. Wang, Guomin,Wang, Kaile,Zhang, Qian,Zhang, Jue,Fang, Jing,Zhu, Ruihao,Yang, Licong,Su, Xia,Yang, Bing,Zhang, Jue,Fang, Jing.

[20]Lowly pathogenic avian influenza (H9N2) infection in Plateau pika (Ochotona curzoniae), Qinghai Lake, China. Yu, Zhijun,Zhang, Kun,Qin, Chuan,Xia, Xianzhu,Yu, Zhijun,Zhang, Kun,Qin, Chuan,Xia, Xianzhu,Yu, Zhijun,Cheng, Kaihui,Sun, Weiyang,Xin, Yue,Li, Lin,Huang, Jing,Sang, Xiaoyu,Li, Xue,Wang, Tiecheng,Qian, Jun,Gao, Yuwei,Xia, Xianzhu,Cai, Jinshan,Ma, Ruilin,Zhao, Quanbang,Cheng, Kaihui,Huang, Jing,Li, Xue.

作者其他论文 更多>>