Identification of a natural recombinant transmissible gastroenteritis virus between Purdue and Miller clusters in China

文献类型: 外文期刊

第一作者: Zhang, Xin

作者: Zhang, Xin;Zhu, Yunnuan;Zhu, Xiangdong;Shi, Hongyan;Chen, Jianfei;Shi, Da;Yuan, Jing;Cao, Liyan;Liu, Jianbo;Dong, Hui;Jing, Zhaoyang;Zhang, Jialin;Wang, Xiaobo;Feng, Li;Dong, Hui

作者机构:

关键词: identification;natural recombinant;transmissible gastroenteritis virus

期刊名称:EMERGING MICROBES & INFECTIONS ( 影响因子:7.163; 五年影响因子:7.329 )

ISSN: 2222-1751

年卷期: 2017 年 6 卷

页码:

收录情况: SCI

摘要: Transmissible gastroenteritis virus (TGEV) is an infective coronavirus (CoV) that causes diarrhea-related morbidity and mortality in piglets. For the first time, a natural recombination strain of a TGEV Anhui Hefei (AHHF) virus between the Purdue and the Miller clusters was isolated from the small intestine content of piglets in China. A phylogenetic tree based on a complete genome sequence placed the TGEV AHHF strain between the Purdue and the Miller clusters. The results of a computational analysis of recombination showed that the TGEV AHHF strain is a natural recombinant strain between these clusters. Two breakpoints located in the open reading frame 1a (ORF1a) and spike (S) genes were identified. The pathogenicity of the TGEV AHHF strain was evaluated in piglets, and the results show that TGEV AHHF is an enteric pathogenic strain. These results provide valuable information about the recombination and evolution of CoVs and will facilitate future investigations of the molecular pathogenesis of TGEV.

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