Identification and functional analysis of a new glyphosate resistance gene from a fungus cDNA library
文献类型: 外文期刊
第一作者: Tao, Bo
作者: Tao, Bo;Shao, Bai-Hui;Qiao, Yu-Xin;Wang, Xiao-Qin;Chang, Shu-Jun;Qiu, Li-Juan
作者机构:
关键词: cDNA library;Aspergillus oryzae RIB40;Glyphosate resistance;Gene screening;MES40;SDS-PAGE
期刊名称:PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY ( 影响因子:3.963; 五年影响因子:4.454 )
ISSN: 0048-3575
年卷期: 2017 年 140 卷
页码:
收录情况: SCI
摘要: Glyphosate is a widely used broad spectrum herbicide; however, this limits its use once crops are planted. If glyphosate-resistant crops are grown, glyphosate can be used for weed control in crops. While several glyphosate resistance genes are used in commercial glyphosate tolerant crops, there is interest in identifying additional genes for glyphosate tolerance. This research constructed a high-quality cDNA library form the glyphosateresistant fungus Aspergillus oryzae RIB40 to identify genes that may confer resistance to glyphosate. Using a medium containing glyphosate (120 mM), we screened several clones from the library. Based on a nucleotide sequence analysis, we identified a gene of unknown function (GenBank accession number: XM_001826835.2) that encoded a hypothetical 344-amino acid protein. The gene was named MES40. Its ORE was amplified to construct an expression vector, pGEX-4T-1-MES40, to express the protein in Escherichia coli BL21. The gene conferred glyphosate tolerance to E. coli ER2799 cells. (C) 2017 Elsevier Inc. All rights reserved.
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