The Rice YGL Gene Encoding an Mg2+-chelatase ChlD Subunit is Affected by Temperature for Chlorophyll Biosynthesis

文献类型: 外文期刊

第一作者: Ruan, Banpu

作者: Ruan, Banpu;Yang, Shenglong;Xu, Zhengjin;Ruan, Banpu;Gao, Zhenyu;Zhao, Juan;Zhang, Bin;Zhang, Anpeng;Hong, Kai;Yang, Shenglong;Jiang, Hongzhen;Liu, Chaolei;Chen, Guang;Peng, Youlin;Dong, Guojun;Guo, Longbiao;Qian, Qian

作者机构:

关键词: Mg2+-chelatase ChlD subunit;Rice;Temperature sensitivity;Yellow-green leaf

期刊名称:JOURNAL OF PLANT BIOLOGY ( 影响因子:2.434; 五年影响因子:2.455 )

ISSN: 1226-9239

年卷期: 2017 年 60 卷 4 期

页码:

收录情况: SCI

摘要: Leaf color-related mutants are important materials to study the chlorophyll biosynthesis and chloroplast development. Here, we isolated and characterized a thermo-sensitive yellow-green leaf mutant, ygl, through mutagenized Japonica rice cultivar Zhonghua11 by EMS. The mutant showed yellow-green leaves only at the seedling stage. Genetic and molecular analyses uncovered that YGL encode an Mg2+-chelatase ChlD subunit, which was a new allele of Chl1/YGL7. Quantitative PCR and GUS staining showed that the YGL transcripts expressed in all tested organs and subcellular localization detected YGL protein in the chloroplasts. The mutant displayed yellow-green leaves at 20 degrees C and normal green leaves at 34 degrees C during the seedling stage. Transcript level of YGL in the ygl mutant was significantly reduced at 20 degrees C, and increased at 34 degrees C compared with the wild-type plant. Furthermore, the expression of chlorophyll biosynthesis and chloroplast development related genes were influenced by the mutation of YGL at different temperature. These findings suggested that the YGL gene not only play a key role in chlorophyll biosynthesis, but is affected at transcript level by temperature in rice.

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