Establishment and Application of a Method for Rapid Determination of Total Sugar Content Based on Colorimetric Microplate
文献类型: 外文期刊
第一作者: Wang, Yonggang
作者: Wang, Yonggang;Jing, Yanjun;Leng, Feifan;Wang, Shiwei;Wang, Fan;Zhuang, Yan;Liu, Xiaofeng;Wang, Yonggang;Jing, Yanjun;Leng, Feifan;Wang, Shiwei;Wang, Fan;Zhuang, Yan;Liu, Xiaofeng;Wang, Xiaoli;Ma, Xueqing
作者机构:
关键词: Microplate;Phenol-sulfuric acid colorimetry;Glucose;Response surface methodology;Methodological evaluation
期刊名称:SUGAR TECH ( 影响因子:1.591; 五年影响因子:1.688 )
ISSN: 0972-1525
年卷期: 2017 年 19 卷 4 期
页码:
收录情况: SCI
摘要: To obtain maximal linear regression correlation coefficient (R-2) of glucose standard curve determinations, single-factor test and response surface methodology were applied to optimize conditions including reaction time, incubation temperature and phenol concentration. For investigating optimization of this method, the effects of different metal ions, amino acids and proteins, respectively, were evaluated. The contents of several polysaccharides were determined by three methods, and it was found that the absorption value at 490 nm of the reaction system (230 mu L) placed in the microplate involving 150 mu L sulfuric acid and 30 mu L 5 % phenol solution was in linear relationship with the concentration of glucose range from 10 to 100 nmol/well at 85 degrees C for 20 min. Our results also suggested that the method had a relative deviation of 0.4 %, and the recovery was between 102.7 and 104.10 %.
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