Neutralization of genotype 2 porcine epidemic diarrhea virus strains by a novel monoclonal antibody

文献类型： 外文期刊

第一作者： Liu, Jianbo

作者： Liu, Jianbo;Shi, Hongyan;Chen, Jianfei;Zhang, Xin;Ji, Zhaoyang;Yuan, Jing;Shi, Da;Cao, Liyan;Zhu, Xiangdong;Dong, Hui;Wang, Xiaobo;Zhang, Jialin;Feng, Li;Liu, Jianbo;Shi, Hongyan;Chen, Jianfei;Zhang, Xin;Ji, Zhaoyang;Yuan, Jing;Shi, Da;Cao, Liyan;Zhu, Xiangdong;Dong, Hui;Wang, Xiaobo;Zhang, Jialin;Feng, Li

作者机构：

关键词： Conformational epitope;Monoclonal antibody;Neutralization test;Porcine epidemic diarrhea virus

期刊名称：VIROLOGY （ 影响因子：3.616； 五年影响因子：3.967 ）

ISSN： 0042-6822

年卷期： 2017 年 507 卷

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV) has two genotypes, G1 and G2. To research the immunogenicity differences of PEDV G1 and G2 genotype strains and obtain a neutralizing monoclonal antibody (mAb), we inoculated specific-pathogen-free BALB/c mice with a newly emerged strain, PEDV-LNCT2. After immunizations, cells from the spleen of the mice were fused with SP2/0 myeloma cells. Following culturing and subcloning, a strain, 1B9, secreting neutralizing antibody, was obtained. The 1B9 mAb neutralized new variant genotype 2 PEDV strains (LNCT2, LNSY, and Rims), but it did not neutralize a genotype 1 PEDV strain (CV777), in vitro. Results showed that the epitope recognized by the 1B9 mAb lies in the spike protein, and that it is a conformational epitope. These findings confirm that allelic differences in the PEDV S gene between the G1 and G2 genotype strains led to changes in the S protein and, thus, differences in its immunogenicity.

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