Determination of Aspergillus pathogens in agricultural products by a specific nanobody-polyclonal antibody sandwich ELISA

文献类型： 外文期刊

第一作者： Wang, Ting

作者： Wang, Ting;Li, Peiwu;Zhang, Qi;Zhang, Wen;Zhang, Zhaowei;Wang, Tong;He, Ting;Wang, Ting;Li, Peiwu;Zhang, Qi;Zhang, Zhaowei;Wang, Tong;He, Ting;Wang, Ting;Li, Peiwu;Zhang, Qi;Zhang, Wen;Wang, Tong;He, Ting;Li, Peiwu;Zhang, Zhaowei;Li, Peiwu;Zhang, Wen

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Aspergillus and its poisonous mycotoxins are distributed worldwide throughout the environment and are of particular interest in agriculture and food safety. In order to develop a specific method for rapid detection of Aspergillus flavus to forecast diseases and control aflatoxins, a nanobody, PO8-VHH, highly reactive to A. flavus was isolated from an immunized alpaca nanobody library by phage display. The nanobody was verified to bind to the components of extracellular and intracellular antigen from both A. flavus and A. parasiticus. To construct a sandwich format immunoassay, polyclonal antibodies against Aspergillus were raised with rabbits. Finally, a highly selective nanobody-polyclonal antibody sandwich enzyme-linked immunosorbent assay was optimized and developed. The results revealed that the detection limits of the two fungi were as low as 1 mu g mL(-1), and that it is able to detect fungal concentrations below to 2 mu g mg(-1) of peanut and maize grains in both artificially and naturally contaminated samples. Therefore, we here provided a rapid and simple method for monitoring Aspergillus spp. contamination in agricultural products.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序