Studies on transfer of Bt gene into Glycine max
文献类型: 外文期刊
第一作者: Su, YH
作者: Su, YH;Wang, HL;Yu, MM;Lu, DY;Guo, SD
作者机构:
关键词: Bt gene;GUS gene;gene-gun bombard;Agrobacterium-mediated transformation;soybean transgenic plant
期刊名称:ACTA BOTANICA SINICA ( 影响因子:0.599; )
ISSN: 0577-7496
年卷期: 1999 年 41 卷 10 期
页码:
收录情况: SCI
摘要: Transgenic plants were regenerated from soybean (Glycine max (L.) Merr.) immature cotyledons bombarded by gene-gun with plasmid pGB14AB which contained Bacillus thuringiensis Berliner insecticidal crystal protein gene (Bt gene land GUS gene, and from cotyledon nodes infected with LBA4404 which contained pGB14AB. Immature cotyledons of "Zhonghuang No. 4" (variety) and 8502 (line) differentiated many more somatic embryos than others. Desiccation treatment of somatic embryos from immature cotyledons of "Zhonghuang No. 4" improved significantly their germination frequency. Preculturing of immature cotyledon,vas beneficial for somatic embryogenesis from the cotyledons infected with Agrobacterium tumefaciens (Smith et Townsend) Conn. High efficiency of transformation is related to selection of genotype and recipient, desiccation treatment of somatic embryos and improvement of transformed system in soybean.
分类号:
- 相关文献
作者其他论文 更多>>
-
QTL analysis for rice grain length and fine mapping of an identified QTL with stable and major effects
作者:Wan, XY;Wan, JM;Jiang, L;Wang, JK;Zhai, HQ;Weng, JF;Wang, HL;Lei, CL;Wang, JL;Zhang, X;Cheng, ZJ;Guo, XP
关键词:
-
Successive chromosome walking by compatible ends ligation inverse PCR
作者:Ren, MZ;Chen, QJ;Li, L;Zhang, R;Guo, SD
关键词:Compatible ends ligation inverse PCR (CELI-PCR);chromosome walking;Gossypium hirsutum
-
Functional analysis of nodulin-like promoter in transgenic cotton plants
作者:Ren, MZ;Chen, QJ;Li, L;Zhang, R;Guo, SD
关键词:cotton plant;functional identification;nodulin-like promoter;predominant expression;reproductive organ
-
Feeding behaviour of Helicoverpa armigera larvae on insect-resistant transgenic cotton and non-transgenic cotton
作者:Zhang, JH;Wang, CZ;Qin, JD;Guo, SD
关键词:Helicoverpa armigera;Bacillus thuringiensis;cowpea trypsin inhibitor;transgenic cotton;feeding behaviour
-
Biological activity and field efficacy of a genetically modified Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus expressing an insect-selective toxin from a chimeric promoter
作者:Sun, XL;Wang, HL;Sun, XC;Chen, XW;Peng, CM;Pan, DM;Jehle, JA;van der Werf, W;Vlak, JM;Hua, Z
关键词:HaSNPV;recombinant baculovifus;p6.9 promoter;AaIT;field efficacy;cotton bollworm
-
Integration and inheritance stability of foreign Bt toxin gene in the bivalent insect-resistant transgenic cotton plants
作者:Xia, LQ;Guo, SD
关键词:Bt toxin gene;the bivalent insect-resistant transgenic cotton plants;integration;copy number;the genetic stability
-
The synthesis of insect-specific neurotoxin gene tox34 and acquirement of transgenic tobacco
作者:Mao, LQ;Guo, SD
关键词:insect-specific neurotoxin;mite;tox34 gene;transgenic tobacco