Studies on transfer of Bt gene into Glycine max

文献类型： 外文期刊

第一作者： Su, YH

作者： Su, YH;Wang, HL;Yu, MM;Lu, DY;Guo, SD

作者机构：

关键词： Bt gene;GUS gene;gene-gun bombard;Agrobacterium-mediated transformation;soybean transgenic plant

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 1999 年 41 卷 10 期

页码：

收录情况： SCI

摘要： Transgenic plants were regenerated from soybean (Glycine max (L.) Merr.) immature cotyledons bombarded by gene-gun with plasmid pGB14AB which contained Bacillus thuringiensis Berliner insecticidal crystal protein gene (Bt gene land GUS gene, and from cotyledon nodes infected with LBA4404 which contained pGB14AB. Immature cotyledons of "Zhonghuang No. 4" (variety) and 8502 (line) differentiated many more somatic embryos than others. Desiccation treatment of somatic embryos from immature cotyledons of "Zhonghuang No. 4" improved significantly their germination frequency. Preculturing of immature cotyledon,vas beneficial for somatic embryogenesis from the cotyledons infected with Agrobacterium tumefaciens (Smith et Townsend) Conn. High efficiency of transformation is related to selection of genotype and recipient, desiccation treatment of somatic embryos and improvement of transformed system in soybean.

分类号：

  • 相关文献

[1]Relationship between Omega as well as the length of 3 ' poly (dA) and efficiency of gene expression. Mao, LQ,Guo, SD. 1998

[2]Gene transfer strategy for kiwifruit to obtain pure transgenic plant through inducing adventitious roots in leaf explants with Agrobacterium tumefaciens. Li, M,Huang, ZG,Han, LX,Zhao, GR,Li, YH. 2003

[3]Measuring gene flow in the cultivation of transgenic cotton (Gossypium hirsutum L.). Bao-Hong Zhang,Xiao-Ping Pan,Teng-Long Guo,Qing-Lian Wang,Todd A. Anderson. 2005

[4]Development of lepidopteran pest-resistant transgenic japonica rice harboring a synthetic cry2A* gene. Jin Yong-mei,Ma Rui,Yu Zhi-jing,Lin Xiu-feng,Wang Ling,Jiang Wen-zhu. 2015

[5]Inheritance and segregation of exogenous genes in transgenic cotton. BAO-HONG ZHANG,TENG-LONG GUO,QING-LIAN WANG. 2000

[6]Development of highly regenerable callus lines and Agrobacterium-mediated transformation of Chinese lawngrass (Zoysia sinica Hance) with a cold inducible transcription factor, CBF1. Li, RF,Wei, JH,Wang, HZ,He, J,Sun, ZY. 2006

[7]Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability. Song, Zhang-yue,Tian, Jing-luan,Fu, Wei-zhe,Li, Lin,Lu, Ling-hong,Zhou, Lian,Shou, Hui-xia,Shan, Zhi-hui,Tang, Gui-xiang. 2013

[8]An efficient and high-throughput protocol for Agrobacterium-mediated transformation based on phosphomannose isomerase positive selection in Japonica rice (Oryza sativa L.). Duan, Yongbo,Li, Hao,Li, Juan,Ni, Dahu,Song, Fengshun,Li, Li,Yang, Jianbo,Duan, Yongbo,Song, Fengshun,Zhai, Chenguang,Mei, Wenqian,Gui, Huaping,Zhang, Wanggen,Li, Hao,Li, Juan,Ni, Dahu. 2012

[9]Generation of Marker- and/or Backbone-Free Transgenic Wheat Plants via Agrobacterium-Mediated Transformation. Wang, Gen-Ping,Yu, Xiu-Dao,Sun, Yong-Wei,Xia, Lan-Qin,Wang, Gen-Ping,Jones, Huw D.. 2016

[10]Mannose selection system used for cucumber transformation. He, Zhengquan,Duan, ZhenZhen,Liang, Wei,Chen, Faju,Yao, Wei,Liang, Hongwei,Yue, Chaoyin,Sun, Zongxiu,Chen, Fan,Dai, Jianwu. 2006

[11]Construction of two Gateway vectors for gene expression in fungi. Zhu, Tingheng,Yang, Xiao,Wang, Kun,Cui, Zhifeng,Wang, Weixia. 2009

[12]Efficient Agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection. Ding Zai-Song,Zhao Ming,Jing Yu-Xiang,Li Liang-Bi,Kuang Ting-Nin. 2006

[13]Expressing a modified cowpea trypsin inhibitor gene to increase insect tolerance against Pieris rapae in Chinese cabbage. Ma, Xiaoli,Pei, Yanxi,Ma, Xiaoli,Zhu, Zhen,Li, Yane,Yang, Guangdong. 2017

[14]Development and Characterization of Transgenic Sugarcane with Insect Resistance and Herbicide Tolerance. Wang, Wen Zhi,Yang, Ben Peng,Feng, Xiao Yan,Cao, Zheng Ying,Feng, Cui Lian,Wang, Jun Gang,Xiong, Guo Ru,Shen, Lin Bo,Zeng, Jun,Zhao, Ting Ting,Zhang, Shu Zhen. 2017

[15]The study on anthocyanin regulatory genes as a visual marker in transformation of common wheat (Triticum aestivum). He Yanli,Zhang Xiaodong,He Yanli,Yang Fengping,Zhang Xiaodong. 2014

[16]Efficient regeneration and genetic transformation platform applicable to five Musa varieties. Liu, Juhua,Gao, Pengzhao,Zhang, Jing,Jia, Caihong,Zhang, Jianbin,Hu, Wei,Xu, Biyu,Jin, Zhiqiang,Sun, Peiguang,Wang, Jiashui,Jin, Zhiqiang,Sun, Xiuxiu. 2017

[17]Approaches to improve the transgenic efficiency and to rescue seedlings from hyperhydricity for rapeseed (Brassica napus). Ding, Yongqiang,Zhang, Dingyu,Yin, Guoying,Wang, Wenjing,Ding, Yongqiang,Zhang, Dingyu,Yin, Guoying,Wang, Wenjing.

[18]Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola. Peng, RH,Yao, QH,Xiong, AS,Cheng, ZM,Li, Y. 2006

[19]Agrobacterium-mediated genetic transformation of peanut and the efficient recovery of transgenic plants. Chen, Mingna,Yang, Qingli,Wang, Tong,Chen, Na,Pan, Lijuan,Chi, Xiaoyuan,Yang, Zhen,Wang, Mian,Yu, Shanlin,Chi, Xiaoyuan. 2015

[20]Construction and genetic analysis of anthocyanin-deficient mutants induced by T-DNA insertion in 'Tsuda' turnip (Brassica rapa). Wang, Xiuzhi,Wang, Yu,Chen, Bowei,Fang, Zhiyuan,Li, Yuhua,Wang, Yu,Li, Yuhua,Wang, Xiuzhi,Kawabata, Saneyuki,Fang, Zhiyuan. 2017

作者其他论文 更多>>

微信小程序