Expression of rabbit defensin NP-1 gene in transgenic tobacco plants and its activity against bacterial wilt
文献类型: 外文期刊
第一作者: Peng, YF
作者: Peng, YF;Cao, GC;Ma, JS;Chen, CX;Zhang, LM;Li, WB;Sun, YR
作者机构:
关键词: bacterial disease-resistance;rabbit defensin;tobacco;transgenic plant
期刊名称:CHINESE SCIENCE BULLETIN ( 影响因子:1.649; 五年影响因子:1.738 )
ISSN: 1001-6538
年卷期: 1998 年 43 卷 18 期
页码:
收录情况: SCI
摘要: A cDNA-derived 200 bp fragment encoding a rabbit defensin NP-1 mature peptide was ligated into the GUS-less pBI121 to yield pBIC-35NP1. Tobacco leaf discs were genetically transformed with an Agrobacterium tumefaciens strain harbouring pBIC-35NP1. Northern blot analysis showed that the defensin NP-1 expression cassette was normally transcribed in transgenic plants. Resistance tests demonstrated that expression of native defensin NP-1 can confer partial resistance to the bacterial wilt pathogen, Ralstonia solanacearum.
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