Advances in the biosafety research of non-target DNA in transgenic plants

文献类型: 外文期刊

第一作者: Ma, RC

作者: Ma, RC;Peng, YF;Guan, HS

作者机构:

关键词: biosafety;35S promoter;marker gene;vector backbone sequence;CRE/lox

期刊名称:ACTA BOTANICA SINICA ( 影响因子:0.599; )

ISSN: 0577-7496

年卷期: 2001 年 43 卷 7 期

页码:

收录情况: SCI

摘要: The biosafety of genetically engineered plants has been of concernment in society and science in recent years. The issue of 35S promoter of CaMV has been contentious because of its wide use in plant genetic engineering. The debate on the safety and potential risks of the 35S promoter will be discussed here. Some of concerns are expressed about the dissemination of antibiotic-resistance genes and vector backbone sequences. Various methods and strategies are currently being developed for the marker gene excision and elimination of vector backbone sequences from transgenic plants. In this review, the CRE/lox system which could get rid of the marker geens and vector backbone sequences will he discussed in detail. Advances in the research of the safety assessment of genetically modified plants using the CRE/lox system will also be described.

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