Generation and analysis of expressed sequence tags from almond (Prunus dulcis Mill.) pistils

文献类型： 外文期刊

第一作者： Jiang, YQ

作者： Jiang, YQ;Ma, RC

作者机构：

关键词： almond;expressed sequence tags;pistil;Prunus dulcis Mill.

期刊名称：SEXUAL PLANT REPRODUCTION （ 影响因子：0.931； 五年影响因子：2.035 ）

ISSN： 0934-0882

年卷期： 2003 年 16 卷 4 期

页码：

收录情况： SCI

摘要： Large-scale single-pass sequencing of expressed sequence tags (ESTs) can give a global picture of the genes involved in the development and function of organs and tissues. Almond (Prunus dulcis Mill. syn. Amygdalus communis L. var. dulcis) is a self-incompatible fruit tree of the family Rosaceae. As an attempt to examine the transcripts expressed during pistil development, we randomly selected and partially sequenced over 1,000 clones from a cDNA library of almond pistils. Analysis of the ESTs using the NCBI non-redundant protein database revealed that 456 (similar to45.4%) have significant similarity to protein coding sequences in the database. Among the 549 cDNAs with no similarity to known protein sequences, 56 clones have significant similarity to nucleotide sequences registered in the databases. Sequences were assembled using StackPACK and the estimated number of genes identified was 716: 121 contigs and 595 singletons. Only eight of the unique cDNA clones correspond to previously isolated gene sequences of P. dulcis. After functional classification of almond ESTs, 'stress resistance and defense' and 'protein synthesis and processing' ESTs were found to be the most numerous. Our EST analysis provides a first picture of the numerous almond genes potentially involved in pistil development. It also contributes to our knowledge about gene expression patterns in pistils while providing an extensive reservoir for gene cloning and genetic mapping in almond and other related fruit trees.

分类号：

  • 相关文献

[1]Comparative proteomic analyses reveal the changes of metabolic features in soybean (Glycine max) pistils upon pollination. Li, Ming,Yang, Pingfang,Li, Ming,Sha, Aihua,Zhou, Xinan.

[2]Pollen Tube Behavior in Inter-specific Cross between Prunus Armeniaca L. and Prunus Amygdalus Batsch.. Sun Haoyuan,Wang Yuzhu,Yang Li,Cai Shengwen. 2011

[3]Development of SSR markers for the phylogenetic analysis of almond trees from China and the Mediterranean region. Xu, Y,Ma, RC,Xie, H,Liu, JT,Cao, MQ. 2004

[4]Identification of Expressed Resistance Gene Analogs from Peanut (Arachis hypogaea L.) Expressed Sequence Tags. Liu, Zhanji,Feng, Suping,Pandey, Manish K.,Chen, Xiaoping,Culbreath, Albert K.,Liu, Zhanji,Feng, Suping,Pandey, Manish K.,Varshney, Rajeev K.,Chen, Xiaoping,Guo, Baozhu. 2013

[5]Differential gene expression of cotton cultivar CCRI24 during somatic embryogenesis. Xiuming Wu,Fuguang Li#2;,Chaojun Zhang,Chuanliang Liu,Xueyan Zhang.

[6]Immune gene discovery by expressed sequence tag (EST) analysis of hemocytes in the ridgetail white prawn Exopalaemon carinicauda. Duan, Yafei,Liu, Ping,Li, Jitao,Li, Jian,Chen, Ping,Duan, Yafei. 2013

[7]DEVELOPMENT AND CHARACTERIZATION OF 41 NOVEL EST-SSR MARKERS FOR PISUM SATIVUM (LEGUMINOSAE). Xu, Sheng-Chun,Gong, Ya-Ming,Hu, Qi-Zan,Zhang, Gu-Wen,Mao, Wei-Hua,Fu, Wan,Xian, Qiang-Qiang,Fu, Wan,Xian, Qiang-Qiang. 2012

[8]Identification of differentially expressed genes in Mongolian sheep ovaries by suppression subtractive hybridization. He, Xiaolong,Wang, Feng,Tian, Chunying,Rong, Weiheng,Liu, Yongbin,He, Xiaolong,Li, Bei. 2012

[9]SSR data mined from expressed sequence tags of phytoparasitic nematodes. Huang, Q. X.,Cheng, X. Y.,Xu, R. M.,Xie, B. Y.. 2010

[10]Erwinia carotovora ssp. Carotovora infection induced "defense lignin" accumulation and lignin biosynthetic gene expression in chinese cabbage (Brassica rapa L. ssp pekinensis). Zhang, Song-He,Yang, Qing,Ma, Rong-Cai. 2007

[11]Progress in expressed sequence tags (EST) project of plant genome. Luo, M,Jia, JZ. 2001

[12]Analysis of ESTs from a Normalized cDNA Library of the Rhizome Tip of Oryza longistaminata. Zhang, Ting,Zhao, Xiuqin,Fu, Binying,Zhang, Ting,Yang, Daichang,Li, Lijuan,Hu, Fengyi.

[13]Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays). Li, Hui-Yong,Wang, Tian-Yu,Shi, Yun-Su,Fu, Jun-Jie,Song, Yan-Chun,Wang, Guo-Ying,Li, Yu.

[14]Porcine skeletal muscle differentially expressed gene CMYA1: isolation, characterization, mapping, expression and association analysis with carcass traits. Xu, X. L.,Xu, X. W.,Pan, P. W.,Yu, M.,Liu, B.,Xu, X. L.,Xu, X. W.,Pan, P. W.,Yu, M.,Liu, B.,Li, K.,Jiang, Z. H.,Rothschild, M. F.,Rothschild, M. F..

[15]Analysis of Resistance to Powdery Mildew in Wheat Based on Expressed Sequence Tags (EST) Technique. Luo, M,Kong, XY,Jiang, T,Jia, C,Zhou, RH,Jia, JZ.

[16]Identification of microsatellites in cattle unigenes. Yan, Qiuliang,Lib, Hongbin,Wei, Caihong,Niu, Lili,Li, Shangang,Du, Lixin,Yan, Qiuliang,Zhang, Yinghan,Guan, Shan. 2008

[17]Construction of a full-length cDNA library and preliminary analysis of expressed sequence tags from lymphocytes of half-pipe snowboarding athletes. Zhao, Y. H.,Zhang, Z. B.,Zhao, C. Q.,Zhang, Y.,Wang, Y. F.,Zhu, Z. Q.,Guan, W. J.. 2015

[18]Construction of a Full-Length Enriched cDNA Library and Preliminary Analysis of Expressed Sequence Tags from Bengal Tiger Panthera tigris tigris. Liu, Changqing,Lu, Taofeng,Li, Xiangchen,Ma, Yuehui,Guan, Weijun,Liu, Changqing,Guo, Yu,Liu, Dan,Zhang, Minghai,Ma, Jianzhang. 2013

[19]PRIMARY ANALYSIS OF THE EXPRESSED SEQUENCE TAGS FROM A FULL-LENGTH ENRICHED cDNA LIBRARY OF SIBERIAN TIGER (PANTHERA TIGRIS ALTAICA). Guo, Y.,Liu, C. Q.,Xu, L. S.,Lu, T. F.,Li, X. C.,Ma, Y. H.,Guan, W. J.,Liu, D.,Ma, J. Z.. 2015

[20]Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug phenacoccus solenopsis. Lang, Kun-Ling,Shen, Chang-Peng,Wan, Fang-Hao,Chu, Dong,Fu, Hai-Bin,Wan, Fang-Hao. 2015

作者其他论文 更多>>

微信小程序