Linkage analysis of a fertility restoring mutant generated from CMS rice

文献类型: 外文期刊

第一作者: Shen, YW

作者: Shen, YW;Guan, ZQ;Lu, J;Zhuang, JY;Zheng, KL;Gao, MW;Wang, XM

作者机构:

关键词: rice (Oryza sativa. L);CMS;fertile revertant;restorer gene;RAPD marker;mapping

期刊名称:THEORETICAL AND APPLIED GENETICS ( 影响因子:5.699; 五年影响因子:5.565 )

ISSN: 0040-5752

年卷期: 1998 年 97 卷 1-2 期

页码:

收录情况: SCI

摘要: DNA polymorphism between a cytoplasmic male-sterile rice line II-32A, the male-fertile maintainer counterpart II-32B, a fertile revertant (T24), as well as two commercial indica restorers, was analyzed with randomly amplified polymorphic DNA (RAPD). A very low degree of polymorphism was found between the revertant T24 and II-32A compared with that of indica rice varieties. This result, together with agronomic and genetic evidence, suggests the revertant to be a product of a nuclear mutation. An analysis of polymorphism between II-32A and the revertant T24 with 510 RAPD decamer primers identified the cosegregating markers OPB07(640) and OPB18(1000) to be linked to a sterile allele of the restoring locus in the revertant T24, at a distance of 5.3 cM. RAPD analysis of a mapping population of Tesanai2/CB with primer OPB07 revealed linkage of OPB07(640) with RG374 (10.8 cM) and RG394 (8.8 cM) on chromosome 1. Thus the restorer gene, designated Rf(5), was tentatively localized between RG374 and RG394 on chromosome 1 and appears to be independent of other mapped restorer genes in rice.

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