The SapA Protein Is Involved in Resistance to Antimicrobial Peptide PR-39 and Virulence of Actinobacillus pleuropneumoniae

文献类型： 外文期刊

第一作者： Xie, Fang

作者： Xie, Fang;Wang, Yalei;Li, Gang;Liu, Shuanghong;Cui, Ning;Liu, Siguo;Wang, Chunlai;Wang, Yalei;Langford, Paul R.

作者机构：

关键词： Actinobacillus pleuropneumoniae;SapA;antimicrobial peptide resistance;PR-39;Virulence

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Antimicrobial peptides are essential to the innate immune defense of the mammal against bacterial infection. However, pathogenic bacteria have evolved multiple strategies to resist and evade antimicrobial peptides, which is vital to bacterial survival and colonization in hosts. PR-39 is a linear porcine antimicrobial peptide containing 39 amino acid residues with a high proline content. Resistance to antimicrobial peptide PR-39 has been observed in Actinobacillus pleuropneumoniae. However, little is known about the factors required for this resistance. In the present study, PR-39 exposure increased the expression of the sapA gene in A. pleuropneumoniae. The sapA gene, which encodes a putative peptide transport periplasmic protein, was deleted from this bacterium. The Delta sapA mutant showed increased sensitivity to PR-39 compared to the wild-type MD12 and complemented P Delta sapA strains. However, the Delta sapA mutant did not exhibit any alterations in outer membrane integrity. Scanning electron microscopy showed that the Delta sapA mutant displayed morphological defects, as indicated by a deformed and sunken shape after PR-39 treatment. In addition, disruption of the SapA protein led to reduced colonization and attenuated virulence of A. pleuropneumoniae in the BALB/c mouse model. Collectively, these data suggest that SapA acts as one mechanism for A. pleuropneumoniae to counteract PR-39-mediated killing. To the best of our knowledge, this is the first study to show a mechanism underlying antimicrobial peptide resistance in A. pleuropneumoniae.

分类号：

  • 相关文献

[1]Deletion of the znuA virulence factor attenuates Actinobacillus pleuropneumoniae and confers protection against homologous or heterologous strain challenge. Yuan, Fangyan,Liu, Zewen,Zhou, Danna,Tian, Yongxiang,You, Wujin,Tan, Yongqiang,Zheng, Chengkun,BinWang,Bei, Weicheng,Liao, Yonghong.

[2]Actinobacillus pleuropneumoniae two-component system QseB/QseC regulates the transcription of PilM, an important determinant of bacterial adherence and virulence. Liu, Jinlin,Hu, Linlin,Xu, Zhuofei,Tan, Chen,Fu, Shulin,Cheng, Hui,Chen, Huanchun,Bei, Weicheng,Liu, Jinlin,Yuan, Fangyan.

[3]Characterization and immunogenicity of an apxIA mutant of Actinobacillus pleuropneumoniae. Xu, Fuzhou,Chen, Xiaoling,Shi, Aihua,Yang, Bing,Wang, Jinluo,Li, Yongqing,Guo, Xin,Blackall, P. J.,Yang, Hanchun. 2006

[4]Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8 Delta clpP/apxIIC confers protection against homologous or heterologous strain challenge. Xie, Fang,Li, Gang,Zhou, Long,Zhang, Yanhe,Cui, Ning,Liu, Siguo,Wang, Chunlai. 2017

[5]Screening of strain-specific Actinobacillus pleuropneumoniae genes using a combination method. Lei, Liancheng,Wang, Jiaqi,Lei, Liancheng,Du, Chongtiao,Yang, Peng,Xie, Fang,Ou, Pingyang,Han, Wenyu.

[6]First Report of Actinobacillus pleuropneumoniae Prevalence in Tibetan Pigs in Tibet, China. Shi, X. C.,Mu, Y.,Shi, X. C.,Zhang, N. Z.,Zhou, D. H.,Xu, M. J.,Wu, S. M.,Zhu, X. Q.,Danba, C.,Ga, G.,Zhu, X. Q.. 2012

[7]Cross Protection Experiment in Mice Immunization with Actinobacillus pleuropneumoniae serotype 7 Genomic Expression Library. Liu, Huifang,Liu, Huifang,Si, Wei,Zhou, Yuanyuan,Wang, Chunlai,Liu, Siguo. 2015

[8]Positive role for rApxIVN in the immune protection of pigs against infection by Actinobacillus pleuropneumoniae. Wang, Chunlai,Wang, Yong,Shao, Meili,Si, Wei,Liu, Huifang,Chang, Yuehong,Peng, Wei,Kong, Xiangang,Liu, Siguo,Shao, Meili.

[9]The Lon protease homologue LonA, not LonC, contributes to the stress tolerance and biofilm formation of Actinobacillus pleuropneumoniae. Xie, Fang,Li, Gang,Zhang, Yanhe,Zhou, Long,Liu, Shuanghong,Liu, Siguo,Wang, Chunlai.

[10]Multiple adaptive amino acid substitutions increase the virulence of a wild waterfowl-origin reassortant H5N8 avian influenza virus in mice. Yu, Zhijun,Sun, Weiyang,Zhang, Xinghai,Xia, Xianzhu,Gao, Yuwei,Xia, Xianzhu,Gao, Yuwei,Cheng, Kaihui,Xia, Xianzhu,Gao, Yuwei. 2018

[11]Isolation and functional analysis of the pathogenicity-related gene VdPR3 from Verticillium dahliae on cotton. Zhang, Ya-Lin,Li, Zhi-Fang,Feng, Zi-Li,Feng, Hong-Jie,Zhao, Li-Hong,Shi, Yong-Qiang,Hu, Xiao-Ping,Zhu, He-Qin.

[12]LuxS/AI-2 in Streptococcus agalactiae reveals a key role in acid tolerance and virulence. Ma, Yanping,Hao, Le,Ke, Hao,Liang, Zhiling,Ma, Jiangyao,Liu, Zhenxing,Li, Yugu. 2017

[13]Genome and Molecular Characterization of a CSFV Strain Isolated from a CSF Outbreak in South China. Shen, Hai-Yan,Wang, Jia-Ying,Dong, Xiao-Ying,Zhao, Ming-Qiu,Kang, Yanmei,Li, Yin-Guang,Pei, Jing-Jing,Liao, Ming,Ju, Chun-Mei,Yi, Lin,Hu, Yongming,Chen, Jin-Ding,Shen, Hai-Yan. 2013

[14]Functional characterization of the gene FoOCH1 encoding a putative alpha-1,6-mannosyltransferase in Fusarium oxysporum f. sp cubense. Li, Min-Hui,Xie, Xiao-Ling,Lin, Xian-Feng,Shi, Jin-Xiu,Ding, Zhao-Jian,Ling, Jin-Feng,Xi, Ping-Gen,Zhou, Jia-Nuan,Jiang, Zi-De,Leng, Yueqiang,Zhong, Shaobin,Ling, Jin-Feng. 2014

[15]Studies on the Complementary Differential Varieties and Local Physiologic Races of Magnaporthe grisea in Sichuan Province. . 2009

[16]Pathogenesis and phylogenetic analyses of canine distemper virus strain ZJ7 isolate from domestic dogs in China. Tan, Bin,Wen, Yong-Jun,Wang, Feng-Xue,Zhang, Shu-Qin,Wang, Xiu-Dong,Hu, Jia-Xin,Shi, Xin-Chuan,Yang, Bo-Chao,Chen, Li-Zhi,Cheng, Shi-Peng,Wu, Hua. 2011

[17]Mycosphere Essays 14: Assessing the aggressiveness of plant pathogenic Botryosphaeriaceae. Manawasinghe, I. S.,Chethana, K. W. T.,Zhang, W.,Yan, J. Y.,Li, X. H.,Manawasinghe, I. S.,Hyde, K. D.,Chethana, K. W. T.,Phillips, A. J. L.,Zhang, W.,Zhao, W. S.. 2016

[18]The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of Zoonotic Streptococcus suis. Zhang, Chunyan,Sun, Wen,Dong, Mengmeng,Liu, Wanquan,Li, Lu,Xu, Zhuofei,Zhou, Rui,Tan, Meifang,Gao, Ting,Li, Lu,Xu, Zhuofei,Zhou, Rui. 2017

[19]Characterization in biological traits of entomopathogenic nematodes isolated from North China. Ma, Juan,Chen, Shulong,Li, Xiuhua,Moens, Maurice,De Clercq, Patrick,Han, Richou,Moens, Maurice. 2013

[20]XocR, a LuxR solo required for virulence in Xanthomonas oryzae pv. oryzicola. Xu, Huiyong,Zhao, Yancun,Liu, Fengquan,Xu, Huiyong,Qian, Guoliang,Liu, Fengquan,Xu, Huiyong,Qian, Guoliang,Liu, Fengquan. 2015

作者其他论文 更多>>

微信小程序