Characterization of Copy Number Variation's Potential Role in Marek's Disease

文献类型: 外文期刊

第一作者: Xu, Lingyang

作者: Xu, Lingyang;He, Yanghua;Ding, Yi;Sun, Guirong;Carrillo, Jose Adrian;Li, Yaokun;Ghaly, Mona M.;Ma, Li;Song, Jiuzhou;Xu, Lingyang;Liu, George E.;Xu, Lingyang;Sun, Guirong;Li, Yaokun;Ghaly, Mona M.;Zhang, Huanmin

作者机构:

关键词: copy number variation;chicken;inbred lines;recombinant congenic strains (RCS);Marek's disease;RNA-sequencing

期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.923; 五年影响因子:6.132 )

ISSN: 1422-0067

年卷期: 2017 年 18 卷 5 期

页码:

收录情况: SCI

摘要: Marek's Disease (MD) is a highly contagious pathogenic and oncogenic disease primarily affecting chickens. Chicken Lines 63 and 72, as well as their recombinant congenic strains (RCS) with varied susceptibility to MD, are ideal models to study the complex mechanisms of genetic resistance to MD. In this study, we investigated copy number variation (CNV) in these inbred chicken lines using the Affymetrix Axiom HD 600 K SNP genotyping array. We detected 393 CNV segments across all ten chicken lines, of which 12 CNVs were specifically identified in Line 72. We then assessed genetic structure based on CNV and observed markedly different patterns. Finally, we validated two deletion events in Line 72 and correlated them with genes expression using qPCR and RNA-seq, respectively. Our combined results indicated that these two CNV deletions were likely to contribute to MD susceptibility.

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