iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A

文献类型： 外文期刊

第一作者： Xu, Wentao

作者： Xu, Wentao;Luo, Yunbo;Huang, Kunlun;Wang, Yan;Wang, Yan;Peng, Xiaoli;Yang, Zhuojun;Zhao, Weiwei;Xu, Wentao;Hao, Junran;Wu, Weihong;Shen, Xiao Li;Luo, Yunbo;Huang, Kunlun;Peng, Xiaoli;Shen, Xiao Li

作者机构：

关键词： ochratoxin A;Arabidopsis thaliana;programmed cell death;mitochondria;iTRAQ;mitoproteomes

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN： 2072-6651

年卷期： 2017 年 9 卷 5 期

页码：

收录情况： SCI

摘要： Ochratoxin A (OTA) is one of the most common and dangerous mycotoxins in the world. Previous work indicated that OTA could elicit spontaneous HR-like lesions formation Arabidopsis thaliana, reactive oxygen species (ROS) play an important role in OTA toxicity, and their major endogenous source is mitochondria. However, there has been no evidence as to whether OTA induces directly PCD in plants until now. In this study, the presence of OTA in Arabidopsis thaliana leaves triggered accelerated respiration, increased production of mitochondrial ROS, the opening of ROS-dependent mitochondrial permeability transition pores and a decrease in mitochondrial membrane potential as well as the release of cytochrome c into the cytosol. There were 42 and 43 significantly differentially expressed proteins identified in response to exposure to OTA for 8 and 24 h, respectively, according to iTRAQ analysis. These proteins were mainly involved in perturbation of the mitochondrial electron transport chain, interfering with ATP synthesis and inducing PCD. Digital gene expression data at transcriptional level was consistent with the cell death induced by OTA being PCD. These results indicated that mitochondrial dysfunction was a prerequisite for OTA-induced PCD and the initiation and execution of PCD via a mitochondrial-mediated pathway.

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