Two single nucleotide polymorphisms sites in alpha 1-AT gene and their association with somatic cell score in Chinese Holstein cows

文献类型： 外文期刊

第一作者： Guo, Xiao-Fei

作者： Guo, Xiao-Fei;Hu, Wen-Ping;Wang, Xiang-Yu;Di, Ran;Liu, Qiu-Yue;Chu, Ming-Xing;Lang, Xian-Zheng;Liu, Xiao-Lin;Li, Qiu-Ling;An, Yong-Fu;Guo, Xiao-Fei

作者机构：

关键词： SNP;alpha 1-AT gene;Polymorphism;Somatic cell score;Chinese Holstein cows

期刊名称：JOURNAL OF BIOLOGICAL RESEARCH-THESSALONIKI （ 影响因子：1.889； 五年影响因子：2.988 ）

ISSN： 2241-5793

年卷期： 2017 年 24 卷

页码：

收录情况： SCI

摘要： Background: Alpha 1-antitrypsin (alpha 1-AT) may affect the susceptibility of mastitis in dairy cattle for its possible role in the protection of lactoferrin from proteolytic degradation in the mammary. Milk somatic cell score (SCS) is a logarithmic transformation of the milk somatic cell count widely used as an index to evaluate mastitis. To study the relationships of alpha 1-AT gene and SCS in Chinese Holstein cows, methods of PCR-SSCP, DNA sequencing, PCR-RFLP, and CRS-PCR technologies were used to detect single nucleotide polymorphisms sites in alpha 1-AT gene. Results: Two polymorphic sites at G5503A and G5746C of alpha 1-AT gene were found. AA (0.3633), AB (0.4644) and BB (0.1723) genotypes were detected at G5503A site, CC (0.3483), CD (0.4906) and DD (0.1611) genotypes were found at G5746C in Chinese Holstein cows. Least squares mean of SCS for individuals with BB genotype was significantly lower than that with AA and AB genotype (p < 0.01), and that with AB genotype was significantly lower than that with AA (p < 0.05). There was no significant difference among individuals with CC, CD and DD genotypes (p > 0.05). Least squares mean of SCS for individuals with BBDD genotype combination were significantly lower than those with AACC and AACD (p < 0.05). Conclusions: Statistical analysis indicated that B allele and BBDD genotype combination of alpha 1-AT can improve mastitis resistance in dairy cattle.

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