Nested-PCR assay for detection of Schistosoma japonicum infection in domestic animals

文献类型： 外文期刊

第一作者： Zhang, Xin

作者： Zhang, Xin;He, Chuan-Chuan;Liu, Jin-Ming;Li, Hao;Lu, Ke;Fu, Zhi-Qiang;Zhu, Chuan-Gang;Hong, Yang;Jin, Ya-Mei;Lin, Jiao-Jiao;Liu, Yi-Ping;Tong, Lai-Bao;Zhou, De-bao;Zha, Li;Lin, Jiao-Jiao

作者机构：

关键词： Schistosomiasis;Domestic animals;Nested-PCR;Diagnosis

期刊名称：INFECTIOUS DISEASES OF POVERTY （ 影响因子：4.52； 五年影响因子：4.307 ）

ISSN： 2049-9957

年卷期： 2017 年 6 卷

页码：

收录情况： SCI

摘要： Background: Schistosomiasis japonica is a common zoonosis. Domestic animals are the primary source of infection and play an important role in disease transmission. The prevalence and infectivity of this disease in domestic animals in China have significantly decreased and, for this reason, diagnostics with a higher sensitivity have become increasingly necessary. It was reported that polymerase chain reaction (PCR)-based methods could be used to detect schistosome infection in humans and animals and presented a high sensitivity and specificity. The present study aimed to develop a PCR-based method for detection of Schistosoma japonicum infection in domestic animals. Methods: A specific nested-PCR assay was developed to detect S. japonicum infection in domestic animals via amplification of a 231-bp DNA fragment of retrotransposon SjR2. The developed assay was first used in sera and dry blood filter paper (DBFP) from goats and buffaloes at different time points of infection. Then, 78 DBFPs from 39 artificially-infected bovines at 14 and 28 days post-infection and 42 DBFPs from schistosome-negative bovines from the city of Huangshan in the Anhui province were used to evaluate the diagnostic validity. Furthermore, this assay was used to detect S. japonicum infection in domestic animals in Dongzhi and Wangjiang counties. Results: The expected PCR product was detected in eggs and adult worms of S. japonicum and blood samples from S. japonicum-infected goats and water buffaloes, but not from Fasciola and Haemonchus contortus worms. The nested-PCR assay could detect the target S. japonicum DNA in DBFPs from goats and buffaloes after day 3 post-infection. The sensitivity in buffaloes at 14 and 28 days post-infection was 92.30% (36/39) and 100% (39/39), respectively. The specificity was 97.60% (41/42). The positivity rates in Dongzhi and Wangjiang counties were 6.00% and 8.00% in bovines and 22.00% and 16.67% in goats, respectively. The positivity rates in goats in both counties were higher than those in bovines with a significant difference in Dongzhi County but not in Wangjiang County (P < 0.05 and P = 0.23, respectively). Conclusions: Our results suggest that the developed nested-PCR assay may be used for the diagnosis of S. japonicum infection in domestic animals, and the control of S. japonicum infection in goats should be paid more attention.

分类号：

  • 相关文献

[1]A candidate recombinant antigen for diagnosis of schistosomiasis japonica in domestic animals. Feng, Jintao,Xu, Rui,Zhang, Xin,Han, Yu,He, Chuanchuan,Lu, Chao,Hong, Yang,Lu, Ke,Li, Hao,Jin, Yamei,Lin, Jiaojiao,Liu, Jinming,Lin, Jiaojiao.

[2]Biotechnological advances in the diagnosis, species differentiation and phylogenetic analysis of Schistosoma spp.. Zhao, Guang-Hui,Li, Juan,Li, Xiao-Yan,Zhu, Xing-Quan,Zhao, Guang-Hui,Li, Juan,Lin, Rui-Qing,Blair, David,Li, Xiao-Yan,Elsheikha, Hany M.,Zou, Feng-Cai,Zhu, Xing-Quan. 2012

[3]First Report of Occurrences of Two Cyprinid Herpesvirus 3 Variants, I++ II- and I++ II+Delta, in China. Liu, Zhenxing,Ke, Hao,Ma, Yanping,Hao, Le,Ma, Jiangyao,Liang, Zhiling,Chen, Zhongjian,Liu, Zhenxing,Ke, Hao,Ma, Yanping,Hao, Le,Ma, Jiangyao,Liang, Zhiling.

[4]Diagnosis of Peste des Petits Ruminants in Wild and Domestic Animals in Xinjiang, China, 2013-2016. Li, J.,Li, L.,Wu, X.,Liu, F.,Zou, Y.,Wang, Q.,Liu, C.,Bao, J.,Wang, Z.,Wang, W.,Ma, W.,Lin, H.,Huang, J.,Zheng, X.. 2017

[5]Progress of genome wide association study in domestic animals. Zhang, Hui,Wang, Zhipeng,Wang, Shouzhi,Li, Hui,Zhang, Hui,Wang, Zhipeng,Wang, Shouzhi,Li, Hui. 2012

[6]Molecular Detection of Tick-Borne Rickettsiales in Goats and Sheep from Southeastern China. Ge, Yan,Yin, Hongmei,Pan, Weiqing,Rikihisa, Yasuko,Yin, Hong. 2016

[7]Evaluation on Infectivity of Babesia microti to Domestic Animals and Ticks Outside the Ixodes Genus. Wu, Jiajun,Cao, Jie,Zhou, Yongzhi,Zhang, Houshuang,Gong, Haiyan,Zhou, Jinlin,Zhou, Jinlin. 2017

[8]Altered levels of circulating miRNAs are associated Schistosoma japonicum infection in mice. Zhu, Lihui,Dao, Jinwei,Du, Xiaoli,Li, Hao,Lu, Ke,Liu, Jinming,Cheng, Guofeng. 2015

[9]Successful outcome of an integrated strategy for the reduction of schistosomiasis transmission in an endemically complex area. Li, Shi-Zhu,Qian, Ying-Jun,Wang, Qiang,Zhou, Xiao-Nong,Yang, Kun,Zhang, Hua-Ming,Liu, Jun,Chen, Mu-Hua,Huang, Xi-Bao,Xu, Yin-Long. 2012

[10]A specific PCR assay for the identification and differentiation of Schistosoma japonicum geographical isolates in mainland China based on analysis of mitochondrial genome sequences. Zhao, Guang-Hui,Li, Juan,Song, Hui-Qun,Li, Xiao-Yan,Zou, Feng-Cai,Zhu, Xing-Quan,Zhao, Guang-Hui,Zhao, Guang-Hui,Li, Juan,Song, Hui-Qun,Chen, Fen,Lin, Rui-Qing,Yuan, Zi-Guo,Weng, Ya-Biao,Li, Xiao-Yan,Zou, Feng-Cai,Zhu, Xing-Quan,Hu, Min,Zhu, Xing-Quan.

[11]Prevention of Schistosoma japonicum infection in mice with long-acting praziquantel implants. Lei, Lei,Guo, Shengrong,Cheng, Liang,Hou, Jingwen,Zhu, Chuangang,Shi, Yaojun,Jiang, Yan,Lin, Jiaojiao. 2012

[12]MicroRNAs: Potentially important regulators for schistosome development and therapeutic targets against schistosomiasis. Cheng, Guofeng,Jin, Youxin,Jin, Youxin. 2012

[13]Role of microRNAs in schistosomes and schistosomiasis. Zhu, Lihui,Liu, Jinming,Cheng, Guofeng. 2014

[14]Changes in microRNA expression in response to Schistosoma japonicum infection. Hong, Y.,Fu, Z.,Cao, X.,Lin, J.,Cao, X.,Lin, J..

[15]In vitro and in vivo evaluation of small interference RNA-mediated gynaecophoral canal protein silencing in Schistosoma japonicum. Cheng, Guofeng,Fu, Zhiqiang,Lin, Jiaojiao,Cai, Youmin,Shi, Yi,Zhou, Yuancong,Jin, Youxin. 2009

[16]Rapid detection and identification of four major Schistosoma species by high-resolution melt (HRM) analysis. Li, Juan,Zhu, Xing-Quan,Li, Juan,Zhao, Guang-Hui,Lin, RuiQing,Blair, David,Sugiyama, Hiromu.

[17]A specific indel marker for the Philippines Schistosoma japonicum revealed by analysis of mitochondrial genome sequences. Li, Juan,Li, Juan,Zhu, Xing-Quan,Chen, Fen,Sugiyama, Hiromu,Blair, David,Lin, Rui-Qing.

[18]Monoclonal antibodies for differentiating infections of three serological-related tospoviruses prevalent in Southwestern China. Chen, Yu-Han,Chien, Wan-Chu,Sun, Jing-Hua,Wang, Yun-Chi,Chen, Tsung-Chi,Dong, Jiahong,Zheng, Kuanyu,Wu, Kuo,Yeh, Shyi-Dong,Yeh, Shyi-Dong,Chen, Tsung-Chi. 2016

[19]Detection of anti-Trichinella antibodies in serum of experimentally-infected swine by immunochromatographic strip. Fu, B. Q.,Li, W. H.,Gai, W. Y.,Yao, J. X.,Qu, Z. G.,Xie, Z. Z.,Wang, Y. H.,Zhang, D. L.,Blaga, R.. 2013

[20]Classical swine fever in China: A minireview. Luo, Yuzi,Li, Su,Sun, Yuan,Qiu, Hua-Ji.

作者其他论文 更多>>

微信小程序