An On-Site, Ultra-Sensitive, Quantitative Sensing Method for the Determination of Total Aflatoxin in Peanut and Rice Based on Quantum Dot Nanobeads Strip

文献类型： 外文期刊

第一作者： Ouyang, Suiyan

作者： Ouyang, Suiyan;Zhang, Zhaowei;He, Ting;Li, Peiwu;Zhang, Qi;Chen, Xiaomei;Wang, Du;Li, Hui;Tang, Xiaoqian;Zhang, Wen;Ouyang, Suiyan;Zhang, Zhaowei;He, Ting;Li, Peiwu;Zhang, Qi;Wang, Du;Ouyang, Suiyan;Zhang, Zhaowei;He, Ting;Li, Peiwu;Li, Hui;Zhang, Wen;Ouyang, Suiyan;Zhang, Zhaowei;Li, Peiwu;Chen, Xiaomei;Tang, Xiaoqian;Zhang, Wen

作者机构：

关键词： total aflatoxin;quantum dot nanobead;test strip;on-site;peanut and rice;agro-food safety

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN： 2072-6651

年卷期： 2017 年 9 卷 4 期

页码：

收录情况： SCI

摘要： An on-site, ultra-sensitive, and quantitative sensing method was developed based on quantum dot nanobeads (QDNBs) and a test strip for the determination of total aflatoxins (AFTs) in rice and peanuts. The monoclonal antibody against AFT (mAb(AFT)) was homemade and labeled with QDNB. After the pre-coating of the AFT antigen on the test line (T line), the competitive immunoreactions were conducted between AFT and AFT antigen on the T line with QDNBs-mAb(AFT). Under optimal conditions, this approach allowed a rapid response towards AFT with a considerable sensitivity of 1.4 pg/mL and 2.9 pg/mL in rice and peanut matrices, respectively. The put-in and put-out durations were within 10 min. The recoveries for AFT in rice and peanut sample matrices were recorded from 86.25% to 118.0%, with relative deviations (RSD) below 12%. The assay was further validated via the comparison between this QDNB strip and the conventional HPLC method using spiked samples. Thus, the design provided a potential alternative for on-site, ultra-sensitive, and quantitative sensing of AFT that could also be expanded to other chemical contaminants for food safety.

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