Isolation and characterisation of a novel antibacterial peptide from a native swine intestinal tract-derived bacterium

文献类型： 外文期刊

第一作者： Xin, Haiyun

作者： Xin, Haiyun;Ji, Shengyue;Peng, Jiayin;Han, Peng;An, Xiaopeng;Wang, Shan;Cao, Binyun;Ji, Shengyue

作者机构：

关键词： Antibacterial peptide;MIC;Tibet swine;Antitumour

期刊名称：INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS （ 影响因子：5.283； 五年影响因子：5.209 ）

ISSN： 0924-8579

年卷期： 2017 年 49 卷 4 期

页码：

收录情况： SCI

摘要： Antimicrobial peptides (AMPs) are highly associated with antipathogenic activity, without generating drug resistance in targeted bacteria. In this study, the existence of AMPs in the Tibetan swine, a China-native, cold-resistant and seldom-sick breed of pig, was investigated. A peptide secreted by a Tibetan swine intestinal tract-derived Bacillus strain was isolated using reversed-phase chromatography (RPC), ultrafiltration and reversed-phase high-performance liquid chromatography (RP-HPLC). The peptide was identified by mass spectrometry and was characterised for activity against Escherichia coli and Staphylococcus aureus. The 16-amino acid peptide (ASVVNKLTGGVAGLLK), named TP, had a molecular mass of 1568.919 Da and exhibited inhibitory activity against Gram-positive and Gram-negative bacteria [minimum inhibitory concentrations (MICs) of 2.5-5 mu M and 10-20 mu M for E. coli and S. aureus, respectively] as well as human MKN-45 and NB4 tumour cell lines [50% inhibitory concentration (IC50) = 4.686 mu M and 11.479 mu M, respectively]. TP also exhibited weak haemolytic activity. Furthermore, TP enhanced cell membrane permeability and K+ outflow, bound with E. coli genomic DNA in vitro and inhibited E. coli growth. Thus, TP represents a strong candidate as an antibacterial peptide. (C) 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

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