Spatiotemporal Transcriptome Analysis Provides Insights into Bicolor Tepal Development in Lilium "Tiny Padhye"

文献类型： 外文期刊

第一作者： Xu, Leifeng

作者： Xu, Leifeng;Yang, Panpan;Feng, Yayan;Xu, Hua;Cao, Yuwei;Tang, Yuchao;Yuan, Suxia;Liu, Xinyan;Ming, Jun;Yang, Panpan

作者机构：

关键词： Lilium spp;transcriptome;bicolor tepals;anthocyanin biosynthesis;chlorophyll metabolism;transcriptional network

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： The bicolor Asiatic hybrid lily cultivar "Tiny Padhye" is an attractive variety because of its unique color pattern. During its bicolor tepal development, the upper tepals undergo a rapid color change from green to white, while the tepal bases change from green to purple. However, the molecular mechanisms underlying these changes remain largely uncharacterized. To systematically investigate the dynamics of the lily bicolor tepal transcriptome during development, we generated 15 RNA-seq libraries from the upper tepals (S2-U) and basal tepals (S1-D, S2-D, S3-D, and S4-D) of Lilium "Tiny Padhye." Utilizing the Illumina platform, a total of 295,787 unigenes were obtained from 713.12 million high-quality paired-end reads. A total of 16,182 unigenes were identified as differentially expressed genes during tepal development. Using Kyoto Encyclopedia of Genes and Genomes pathway analysis, candidate genes involved in the anthocyanin biosynthetic pathway (61 unigenes), and chlorophyll metabolic pathway (106 unigenes) were identified. Further analyses showed that most anthocyanin biosynthesis genes were transcribed coordinately in the tepal bases, but not in the upper tepals, suggesting that the bicolor trait of "Tiny Padhye" tepals is caused by the transcriptional regulation of anthocyanin biosynthetic genes. Meanwhile, the high expression level of chlorophyll degradation genes and low expression level of chlorophyll biosynthetic genes resulted in the absence of chlorophylls from "Tiny Padhye" tepals after flowering. Transcription factors putatively involved in the anthocyanin biosynthetic pathway and chlorophyll metabolism in lilies were identified using a weighted gene co-expression network analysis and their possible roles in lily bicolor tepal development were discussed. In conclusion, these extensive transcriptome data provide a platform for elucidating the molecular mechanisms of bicolor tepals in lilies and provide a basis for similar research in other closely related species.

分类号：

  • 相关文献

[1]Transcriptome Analysis Identifies Key Candidate Genes Mediating Purple Ovary Coloration in Asiatic Hybrid Lilies. Xu, Leifeng,Yang, Panpan,Yuan, Suxia,Feng, Yayan,Xu, Hua,Cao, Yuwei,Ming, Jun,Yang, Panpan. 2016

[2]Transcriptome Profiling of Light-Regulated Anthocyanin Biosynthesis in the Pericarp of Litch. Zhang, Hong-Na,Li, Wei-Cai,Shi, Sheng-You,Shu, Bo,Liu, Li-Qin,Wei, Yong-Zan,Xie, Jiang-Hui,Wang, Hui-Cong. 2016

[3]Identification of Candidate Anthocyanin-Related Genes by Transcriptomic Analysis of `Furongli' Plum (Prunus salicina Lindl.) during Fruit Ripening Using RNA-Seq. Fang, Zhi-Zhen,Zhou, Dan-Rong,Ye, Xin-Fu,Jiang, Cui-Cui,Pan, Shao-Lin. 2016

[4]ES7, encoding a ferredoxin-dependent glutamate synthase, functions in nitrogen metabolism and impacts leaf senescence in rice. Bi, Zhenzhen,Zhang, Yingxin,Wu, Weixun,Zhan, Xiaodeng,Yu, Ning,Xu, Tingting,Liu, Qunen,Li, Zhi,Shen, Xihong,Chen, Daibo,Cheng, Shihua,Cao, Liyong,Bi, Zhenzhen,Zhang, Yingxin,Wu, Weixun,Zhan, Xiaodeng,Yu, Ning,Xu, Tingting,Liu, Qunen,Li, Zhi,Shen, Xihong,Chen, Daibo,Cheng, Shihua,Cao, Liyong.

[5]Identification of anthocyanin biosynthesis related microRNAs in a distinctive Chinese radish (Raphanus sativus L.) by high-throughput sequencing. Sun, Yuyan,Qiu, Yang,Duan, Mengmeng,Wang, Jinglei,Zhang, Xiaohui,Wang, Haiping,Song, Jiangping,Li, Xixiang,Sun, Yuyan.

[6]The apple WD40 protein MdTTG1 interacts with bHLH but not MYB proteins to regulate anthocyanin accumulation. An, Xiu-Hong,Tian, Yi,Chen, Ke-Qin,Wang, Xiao-Fei,Hao, Yu-Jin,An, Xiu-Hong,Tian, Yi,Chen, Ke-Qin,Wang, Xiao-Fei,Hao, Yu-Jin,An, Xiu-Hong,Tian, Yi,Chen, Ke-Qin,Wang, Xiao-Fei,Hao, Yu-Jin,Tian, Yi. 2012

[7]Anthocyanin Biosynthesis Regulation in the Fruit of Citrus sinensis cv. Tarocco. Liu, Jian-jun,Chen, Ke-ling,Li, Hong-wen,He, Jian,Guan, Bin,He, Li,Wang, Jian-hui,Liu, Jian-jun,Chen, Ke-ling,Li, Hong-wen,He, Jian,Guan, Bin,He, Li,Wang, Jian-hui,Liu, Jian-jun,Chen, Ke-ling,Li, Hong-wen,He, Jian,Guan, Bin,He, Li.

[8]Accumulation and Molecular Regulation of Anthocyanin in Purple Tumorous Stem Mustard (Brassica juncea var. tumida Tsen et Lee). Xie, Qiaoli,Hu, Zongli,Zhang, Yanjie,Zhao, Zhiping,Chen, Guoping,Tian, Shibing,Wang, Zhijin,Yang, Yang.

[9]A collection of 10,096 indica rice full-length cDNAs reveals highly expressed sequence divergence between Oryza sativa indica and japonica subspecies. Liu, Xiaohui,Lu, Tingting,Yu, Shuliang,Li, Ying,Huang, Yuchen,Huang, Tao,Zhang, Lei,Zhu, Jingjie,Zhao, Qiang,Fan, Danlin,Mu, Jie,Shangguan, Yingying,Feng, Qi,Guan, Jianping,Ying, Kai,Zhang, Yu,Lin, Zhixin,Sun, Zongxiu,Qian, Qian,Lu, Yuping,Han, Bin.

[10]Transcriptome analysis of the roots at early and late seedling stages using Illumina paired-end sequencing and development of EST-SSR markers in radish. Wang, Shufen,He, Qiwei,Liu, Xianxian,Xu, Wenling,Li, Libin,Gao, Jianwei,Wang, Fengde,Wang, Xiufeng. 2012

[11]Transcriptome Analysis of Calcium and Hormone-Related Gene Expressions during Different Stages of Peanut Pod Development. Li, Yan,Meng, Jingjing,Yang, Sha,Guo, Feng,Zhang, Jialei,Geng, Yun,Cui, Li,Li, Xinguo,Wan, Shubo. 2017

[12]Transcriptome profiling of peanut gynophores revealed global reprogramming of gene expression during early pod development in darkness. Xia, Han,Zhao, Chuanzhi,Hou, Lei,Li, Aiqin,Zhao, Shuzhen,Bi, Yuping,An, Jing,Wan, Shubo,Wang, Xingjun,Bi, Yuping,Wan, Shubo,Wang, Xingjun,Bi, Yuping,An, Jing,Zhao, Yanxiu,Wang, Xingjun. 2013

[13]Switch on a more efficient pyruvate synthesis pathway based on transcriptome analysis and metabolic evolution. Yang, Maohua,Mu, Tingzhen,Xing, Jianmin,Chen, Ruonan,Zhang, Xiang. 2017

[14]Transcriptome analysis of rosette and folding leaves in Chinese high-throughput RNA sequencing. Wang, Fengde,Li, Libin,Li, Huayin,Liu, Lifeng,Zhang, Yihui,Gao, Jianwei,Wang, Xiaowu. 2012

[15]Transcriptome analysis reveals long noncoding RNAs involved in fiber development in cotton (Gossypium arboreum). Changsong Zou,Qiaolian Wang,Cairui Lu,Wencui Yang,Youping Zhang,Hailiang Cheng,Xiaoxu Feng,Mtawa Andrew Prosper,Guoli Song. 2016

[16]De novo assembly and transcriptome analysis of two contrary tillering mutants to learn the mechanisms of tillers outgrowth in switchgrass (Panicum virgatum L.). Kaijie Xu,Fengli Sun,Guaiqiang Chai,Yongfeng Wang,Lili Shi,Shudong Liu,Yajun Xi. 2015

[17]Global analysis of the Gossypium hirsutum L. Transcriptome during leaf senescence by RNA-Seq. Min Lin,Chaoyou Pang,Shuli Fan,Meizhen Song,Hengling Wei,Shuxun Yu. 2015

[18]PAG1, a cotton brassinosteroid catabolism gene, modulates fiber elongation. Yang, Zuoren,Zhang, Chaojun,Yang, Xiaojie,Liu, Kun,Wu, Zhixia,Zhang, Xueyan,Zheng, Wu,Liu, Chuanliang,Lu, Lili,Yang, Zhaoen,Qian, Yuyuan,Xu, Zhenzhen,Li, Changfeng,Li, Fuguang,Xun, Qingqing,Li, Jia.

[19]Identification of candidate thermotolerance genes during early seedling stage in upland cotton (Gossypium hirsutum L.) revealed by comparative transcriptome analysis. Peng, Zhen,Cao, Moju,Xu, Jie,Lu, Yanli,Peng, Zhen,He, Shoupu,Gong, Wenfang,Sun, Junling,Pan, Zhaoe,Du, Xiongming,Sun, Gaofei.

[20]Characterization of the global transcriptome for cotton (Gossypium hirsutum L.) anther and development of SSR marker. Xianwen Zhang ,Zhenwei Ye,TiankangWang,Hairong Xiong,Xiaoling Yuan,Zhigang Zhang,Youlu Yuan,Zhi Liu.

作者其他论文 更多>>

微信小程序