Bis(sulfosuccinimidyl) suberate (BS3) crosslinking analysis of the behavior of amyloid-beta peptide in solution and in phospholipid membranes

文献类型： 外文期刊

第一作者： Shi, Jing-Ming

作者： Shi, Jing-Ming;Liu, En-Qi;Zhang, Lin;Shi, Jing-Ming;Pei, Jie

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2017 年 12 卷 3 期

页码：

收录情况： SCI

摘要： The structure and state of amyloid-beta peptide (A beta) oligomers often need to be checked by reliable experimental methods. Electrophoresis is a commonly applied measurement method. However, due to the presence of detergents, oligomers are easily broken during electrophoresis, which makes it very hard to accurately assess A beta aggregate states. In the current study, bis(sulfosuccinimidyl) suberate (BS3) was used to cross-link A beta 1-42 oligomers prior to electrophoresis. When compared to a previously reported A beta cross-linking agent, glutaraldehyde, it was quite apparent that BS3 is more suitable for detecting intra-membrane A beta oligomers and extra-membrane A beta oligomers states. As such, our findings provide an efficient method for analyzing A beta proteins or other proteins that are easily aggregated in solution and in phospholipid membranes.

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