Development of Near-sogenic Lines in a Parthenogenetically Reproduced Thrips Species, Frankliniella occidentalis
文献类型: 外文期刊
第一作者: Yuan, Guangdi
作者: Yuan, Guangdi;Wan, Yanran;Li, Xiaoyu;He, Bingqing;Zhang, Youjun;Xu, Baoyun;Wang, Shaoli;Xie, Wen;Wu, Qingjun;Zhou, Xuguo
作者机构:
关键词: Frankliniella occidentalis;near-isogenic lines;insecticide resistance;spinosad;ISSR
期刊名称:FRONTIERS IN PHYSIOLOGY ( 影响因子:4.566; 五年影响因子:4.804 )
ISSN: 1664-042X
年卷期: 2017 年 8 卷
页码:
收录情况: SCI
摘要: Although near-isogenic lines (NILs) can standardize genetic backgrounds among individuals, it has never been applied in parthenogenetically reproduced animals. Here, through multiple rounds of backcrossing and spinosad screening, we generated spinosad resistant NILs in the western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), with a haplo-diploid reproduction system. The resultant F. occidentalis NIL-R strain maintained a resistance ratio over 30,000-fold, which was comparable to its parental resistant strain, Spin-R. More importantly, F. occidentalis NIL-R shared 98.90% genetic similarity with its susceptible parental strain Ivf03. By developing this toolset, we are able to segregate individual resistance and facilitate the mechanistic study of insecticide resistances in phloem-feeding arthropods, a group of devastating pest species reproducing sexually as well as asexually.
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