First Characterization of Sphingomyeline Phosphodiesterase Expression in the Bumblebee, Bombus lantschouensis

文献类型： 外文期刊

第一作者： Han, L.

作者： Han, L.;Dong, J.;Wang, Y.;Huang, J.;Wu, J.;Han, L.;He, S.

作者机构：

关键词： Bombus lantschouensis;SMPDgene;gene clone;prokaryotic expression;polyclonal antibody;expression characteristics

期刊名称：SOCIOBIOLOGY （ 影响因子：0.983； 五年影响因子：1.01 ）

ISSN： 0361-6525

年卷期： 2017 年 64 卷 1 期

页码：

收录情况： SCI

摘要： The bumblebee (Bombus lantschouensis Vogt) is an important pollinator of wild plants. Sphingomyelin phosphodiesterase (SMPD) is a hydrolase that plays a major role in sphingolipid metabolism reactions. We report the preparation and characterization of a polyclonal antibody for bumblebee SMPD. We then use the polyclonal antiserum to detect the SMPD protein at different development stages and in different tissues. Our results showed that a 1228bp fragment homologous with the B. terrestris SMPD gene was successfully amplified. The molecular weight of the fusion protein was about 70kDa by SDS-PAGE. An effective polyclonal antibody against SMPD was also obtained from mice and found to have a higher specificity for bumblebee SMPD. Western blotting detection showed that SMPD was expressed at a high level in queen ovaries, although expression was lower in the midgut and venom gland. SMPD expression decreased from the egg stage until the pbl pupal stage. We interpret our results as showing that the development of an effective polyclonal antiserum for the SMPD protein of a bumblebee, which provides a tool for exploring the function of the SMPD gene. In addition, the work has confirmed that SMPD should be considered as an important enzyme during bumblebee egg and larval stages.

分类号：

  • 相关文献

[1]Transcript expression bias of phosphatidylethanolamine binding protein gene in bumblebee, Bombus lantschouensis (Hymenoptera: Apidae). Dong, Jie,Han, Lei,Wang, Ye,Huang, Jiaxing,Wu, Jie.

[2]Functional Characteristics of a Novel Chemosensory Protein in the Cotton Bollworm Helicoverpa armigera (Hubner). Zhang Yong-jun,Guo Yu-yuan,Wang Wei-xuan,Zhang Zi-ding. 2013

[3]Expression analysis of two reverse duplicated small heat shock protein genes in rice (Oryza sativa L.). Guo Hong-xia,Zeng Wen-zhi,Feng Jing-lei,Tang Hui-wu,Bai Mei,Liu Yao-guang,Guo Jing-xin,Guo Hong-xia,Wang Chuang-yun,Zhao Li,Wang Lu-jun,Fan Tao. 2016

[4]Construction of early human embryo cDNA libraries and screening objective genes. Gou, DM,Huang, J,Li, WX,Mao, X,Jiang, DH,Hua, WJ,Fan, JH. 1999

[5]Molecular cloning and mRNA expression of a hepcidin gene from the spinyhead croaker, Collichthys lucidus. Sang, C.,Lin, Y.,Jiang, K.,Zhang, F.,Song, W.. 2015

[6]Cloning of Fructokinase Gene from Longan Fruit and Bioinformatics Analysis. Shuai, L.,Li, J.,Wu, Z. X.,Han, D. M.. 2014

[7]Cloning and Characterization of karmoisin Homologue Gene (Nlka) in Two Brown Planthopper Strains with Different Eye Colors. Liu Shu-hua,Wu Jin-cai,Liu Shu-hua,Tang Jian,Luo Ju,Yang Bao-jun,Wang Ai-ying. 2016

[8]Isolation of the P5CS gene from reed canary grass and its expression under salt stress. Cong, L. L.,Yang, F. Y.,Liu, S. J.,Zhang, Y. W.,Cong, L. L.,Cong, L. L.,Zhang, X. Q.. 2014

[9]Identification and characterization of the DNA replication origin recognition complex gene family in the silkworm Bombyx mori. Yang, Hui-Peng,Zhang, Yao-Zhou,Yang, Hui-Peng,Luo, Su-Juan,Li, Yi-Nu,Zhang, Zhi-Fang.

[10]Cloning and sequence analysis of sucrose phosphate synthase gene from varieties of Pennisetum species. Li, H. C.,Lu, H. B.,Yang, F. Y.,Liu, S. J.,Zhang, Y. W.,Li, H. C.,Lu, H. B.,Yang, F. Y.,Liu, S. J.,Zhang, Y. W.,Bai, C. J.. 2015

[11]Low-affinity iron transport protein Uvt3277 is important for pathogenesis in the rice false smut fungus Ustilaginoidea virens. Zheng, Meng-ting,Ding, Hui,Huang, Lei,Wang, Ya-hui,Yu, Mi-na,Zheng, Rui,Yu, Jun-jie,Liu, Yong-feng,Zheng, Meng-ting,Ding, Hui,Huang, Lei,Liu, Yong-feng.

[12]Cloning and Alternative Splicing Analysis of Bombyx mori Transformer-2 Gene using Silkworm EST Database. Niu, BL,Meng, ZQ,Tao, YZ,Lu, SL,Weng, HB,He, LH,Shen, WF.

[13]An Indirect Enzyme-Linked Immunosorbent Assay for Determination of New Herbicide H-9201 in Water. Zhang Cun-Zheng,Liu Xian-Jin,Yu Jie,Zhang Cun-Zheng,Liu Xian-Jin,Yu Jie,Yang Chun-Long,Chen Min. 2011

[14]Expression, purification and characterization of a functional extracellular domain of porcine Fc gamma RII. Tian, Xiaohui,Qiao, Songlin,Zhang, Gaiping,Xi, Jun,Liu, Yunchao,Tian, Xiaohui,Wang, Aiping,Li, Xuewu,Xu, Yuqing. 2009

[15]Development of an antigen-capture ELISA for the detection of equine influenza virus nucleoprotein. Ji, Yuanyuan,Guo, Wei,Zhao, Liping,Li, Hongmei,Lu, Gang,Wang, Zheng,Wang, Guibin,Liu, Cuiyun,Xiang, Wenhua. 2011

[16]Prepared polyclonal antibody of the semicarbazide. Huang, Xuan-yun,Huang, Dong-mei,Cai, You-qiong. 2015

[17]Development of an enzyme-linked immunosorbent assay for podophyllotoxin. Xu, DM,Yu, XY,Liu, YQ,Feng, JT,Pan, LG,Liu, XJ,He, J,Zhang, X. 2005

[18]Development of an antigen-capture ELISA for the detection of avian leukosis virus p27 antigen. Yun, Bingling,Li, Delong,Zhu, Haibo,Liu, Wen,Qin, Liting,Liu, Zaisi,Wu, Guan,Wang, Yongqiang,Qi, Xiaole,Gao, Honglei,Wang, Xiaomei,Gao, Yulong. 2013

[19]Prokaryotic expression and immunoassay of grass carp reovirus capsid VP6 protein. Zhou, Y.,Fan, Y. D.,Zeng, L. B.,Ma, J.. 2013

[20]Effect of hapten structures on specific and sensitive enzyme-linked immunosorbent assays for N-methylcarbamate insecticide metolcarb. Zhang, Qi,Wu, Yunru,Wang, Limin,Hu, Baishi,Liu, Fengquan,Zhang, Qi,Li, Peiwu.

作者其他论文 更多>>

微信小程序