First Characterization of Sphingomyeline Phosphodiesterase Expression in the Bumblebee, Bombus lantschouensis

文献类型： 外文期刊

第一作者： Han, L.

作者： Han, L.;Dong, J.;Wang, Y.;Huang, J.;Wu, J.;Han, L.;He, S.

作者机构：

关键词： Bombus lantschouensis;SMPDgene;gene clone;prokaryotic expression;polyclonal antibody;expression characteristics

期刊名称：SOCIOBIOLOGY （ 影响因子：0.983； 五年影响因子：1.01 ）

ISSN： 0361-6525

年卷期： 2017 年 64 卷 1 期

页码：

收录情况： SCI

摘要： The bumblebee (Bombus lantschouensis Vogt) is an important pollinator of wild plants. Sphingomyelin phosphodiesterase (SMPD) is a hydrolase that plays a major role in sphingolipid metabolism reactions. We report the preparation and characterization of a polyclonal antibody for bumblebee SMPD. We then use the polyclonal antiserum to detect the SMPD protein at different development stages and in different tissues. Our results showed that a 1228bp fragment homologous with the B. terrestris SMPD gene was successfully amplified. The molecular weight of the fusion protein was about 70kDa by SDS-PAGE. An effective polyclonal antibody against SMPD was also obtained from mice and found to have a higher specificity for bumblebee SMPD. Western blotting detection showed that SMPD was expressed at a high level in queen ovaries, although expression was lower in the midgut and venom gland. SMPD expression decreased from the egg stage until the pbl pupal stage. We interpret our results as showing that the development of an effective polyclonal antiserum for the SMPD protein of a bumblebee, which provides a tool for exploring the function of the SMPD gene. In addition, the work has confirmed that SMPD should be considered as an important enzyme during bumblebee egg and larval stages.

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