Transcriptome analysis of Cucumis sativus infected by Cucurbit chlorotic yellows virus

文献类型: 外文期刊

第一作者: Sun, Xinyan

作者: Sun, Xinyan;Wang, Zhenyue;Li, Honglian;Han, Weili;Shi, Yan;Gu, Qinsheng

作者机构:

关键词: CCYV;Transcriptome;Cucumber;RNA-Seq

期刊名称:VIROLOGY JOURNAL ( 影响因子:4.099; 五年影响因子:3.719 )

ISSN: 1743-422X

年卷期: 2017 年 14 卷

页码:

收录情况: SCI

摘要: Background: Cucurbit chlorotic yellows virus (CCYV) is a recently reported bipartite crinivirus that causes chlorotic leaf spots and yellowing symptoms on the leaves of cucurbit plants. The virus-host interaction of CCYV remains to be elucidated, and the influence of criniviruses on the host gene transcriptome requires analysis. Methods: We used transcriptome sequencing to analyse the differentially expressed genes (DEGs) caused by CCYV infection. Results: CCYV infection resulted in 865 DEGs. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis identified 67 pathways, and the three major enrichment pathways (according to the P-values) were photosynthesis-antenna proteins (KO00196), phenylalanine metabolism (KO00360a), and phenylpropanoid biosynthesis (KO00940). Of the 13 DEGs identified in phenylalanine metabolism, 11 genes encode disease resistance-related phenylalanine ammonia-lyase (PAL) genes. Using quantitative real-time PCR, we validated the differential expression of 12 genes. Conclusions: Our study based on the CCYV-cucumber interaction provides comprehensive transcriptomic information, and will improve our understanding of host-crinivirus interactions.

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