Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8 Delta clpP/apxIIC confers protection against homologous or heterologous strain challenge

文献类型： 外文期刊

第一作者： Xie, Fang

作者： Xie, Fang;Li, Gang;Zhou, Long;Zhang, Yanhe;Cui, Ning;Liu, Siguo;Wang, Chunlai

作者机构：

关键词： Actinobacillus pleuropneumoniae;Live attenuated vaccine;Porcine pleuropneumonia

期刊名称：BMC VETERINARY RESEARCH （ 影响因子：2.741； 五年影响因子：2.955 ）

ISSN： 1746-6148

年卷期： 2017 年 13 卷

页码：

收录情况： SCI

摘要： Background: Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, which leads to large economic losses to the swine industry worldwide. In this study, S-8(Delta)clpP(Delta)apxIIC, a double-deletion mutant of A. pleuropneumoniae was constructed, and its safety and protective efficacy were evaluated in pigs. Results: The S-8(Delta)clpP(Delta)apxIIC mutant exhibited attenuated virulence in a murine (BALB/c) model, and caused no detrimental effects on pigs even at a dose of up to 1.0 x 10(9) CFU. Furthermore, the S-8(Delta)clpP(Delta)apxIIC mutant was able to induce a strong immune response in pigs, which included high levels of IgG1 and IgG2, stimulated gamma interferon (IFN-gamma), interleukin 12 (IL-12), and interleukin 4 (IL-4) production, and conferred effective protection against the lethal challenge with A. pleuropneumoniae serovars 7 or 5a. The pigs in the S-8(Delta)clpP(Delta)apxIIC immunized groups have no lesions and reduced bacterial loads in the lung tissue after challenge. Conclusions: The data obtained in this study suggest that the S-8(Delta)clpP(Delta)apxIIC mutant can serve as a highly immunogenic and potential live attenuated vaccine candidate against A. pleuropneumoniae infection.

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