Simultaneously Testing Deoxynivalenol, T2 Toxin and Ochratoxin A in Wheat with a Colloidal Gold Test Strip and Matrix Solid-Phase Dispersion Cleaning

文献类型： 外文期刊

第一作者： Jin, Nuo

作者： Jin, Nuo;Wang, Miao;Fan, Bei;He, Yan;Huang, Yatao;Wang, Fengzhong;Liu, Yang;Wang, Miao;Fan, Bei;He, Yan;Huang, Yatao;Wang, Fengzhong

作者机构：

关键词： Deoxynivalenol;T2 toxin;Ochratoxin A;Colloidal gold;Immunoassay;Matrix solid-phase dispersion cleaning

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2017 年 19 卷 6 期

页码：

收录情况： SCI

摘要： Deoxynivalenol (DON, also called as Vomitoxin), T2 toxin and Ochratoxin A (OTA) are three fungal toxins commonly found in corn or wheat. Chromatography and Mass spectrometer are main analysis methods for testing these mycotoxins. But these methods are time consuming, difficult to operate, and high cost on human resources and economy. Immune Colloidal Gold Assay (ICGA) is a rapid testing method suitable for on-spot testing. Unfortunately, the main reason for ICGA difficult to test these three toxins simultaneously is the optimization of the testing condition and sample preparation procedures. In this study, by utilizing three different coating antigens with different concentration, three test lines were set (T-1, T-2, T-3), for detecting DON, T2 toxin and OTA, respectively. The Test Line disappears when the concentration reach 1 mg/kg (for DON), 1 mg/kg (for T2) and 5 mu g/kg (for OTA), separately. We also developed a sample preparation method by using MSPD for extraction and cleaning of the three toxins above for the simultaneously ICGA. That makes it easier for detecting toxins in wheat and for assurance of the food that can meet official limit of residue restrictions of China. The method could obviously improve the people's food safety and human health in real practice of agro-products' processing and purchasing. (C) 2017 Friends Science Publishers

分类号：

  • 相关文献

[1]Development of an immunochromatographic test strip for simultaneous qualitative and quantitative detection of ochratoxin A and zearalenone in cereal. Sun, Yaning,Xing, Guangxu,Yang, Jifei,Wang, Fangyu,Deng, Ruiguang,Zhang, Gaiping,Hu, Xiaofei,Sun, Yaning,Zhang, Yong,Zhang, Gaiping.

[2]Occurrence of mycotoxins in feed ingredients and complete feeds obtained from the Beijing region of China. Zhao, Lihong,Fan, Yu,Ma, Shanshan,Ji, Cheng,Ma, Qiugang,Zhang, Jianyun,Jia, Yaxiong,Sun, Lei.

[3]Occurrence of four mycotoxins in cereal and oil products in Yangtze Delta region of China and their food safety risks. Li, Rui,Wang, Xu,Zhou, Yu,Zhou, Ting,Yang, Dongxu,Wang, Qi. 2014

[4]Self-paired monoclonal antibody lateral flow immunoassay strip for rapid detection of Acidovorax avenae subsp citrulli. Zeng, Haijuan,Li, Jianwu,Zhai, Xuzhao,Song, Chunmei,Liu, Qing,Guo, Wenbo,Liang, Beibei,Zhao, Wenjun,Fan, Enguo.

[5]Development of a lateral flow immunoassay strip for screening of sulfamonomethoxine residues. Zhang, G.,Wang, X.,Bao, Y.,Wang, Z.,Zhang, G.,Wang, X.,Yang, Y.,Luo, J.,Li, Q.,Guo, J.,Yang, J.,Xing, G.,Chai, S.,Shi, T.,Liu, Q.,Zhi, A.,Qu, M.. 2008

[6]Rapid and Sensitive Detection of the Food Allergen Glycinin in Powdered Milk Using a Lateral Flow Colloidal Gold Immunoassay Strip Test. Wang, Yao,Li, Zhixi,Deng, Ruiguang,Zhang, Gaiping,Li, Qingmei,Yang, Jifei,Sun, Yaning,Hu, Xiaofei,Zhang, Gaiping.

[7]Development of an Immunochromatographic Strip Test for the Rapid Detection of Zearalenone in Corn. Sun, Yaning,Hu, Xiaofei,Yang, Jifei,Wang, Fangyu,Wang, Yao,Deng, Ruiguang,Zhang, Gaiping,Sun, Yaning,Zhang, Yong,Zhang, Gaiping.

[8]Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Olaquindox Residues. Song, Chunmei,Liu, Qingtang,Zhi, Aimin,Yang, Jifei,Zhi, Yubao,Li, Qingmei,Hu, Xiaofei,Deng, Ruiguang,Zhang, Gaiping,Song, Chunmei,Casas, Justin,Tang, Liang.

[9]Establishment of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Soybean Allergen beta-Conglycinin. Wang, Yao,Li, Zhaozhou,Pei, Yafeng,Hou, Yuze,Wang, Yao,Li, Qingmei,Sun, Yaning,Yang, Jifei,Yang, Yanyan,Zhi, Yubao,Deng, Ruiguang,Hu, Xiaofei.

[10]Development of a colloidal gold-based strip test for the detection of chlorothalonil residues in cucumber. Yang, Xingdong,Zeng, Xianyin,Yang, Xingdong,Zhang, Gaiping,Wang, Fangyu,Wang, Yinbiao,Hu, Xiaofei,Li, Qingmei,Jia, Guochao,Liu, Zhen,Wang, Yao,Deng, Ruiguang,Zhang, Gaiping.

[11]Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Enrofloxacin Residues. Zhao, Yinli,Zhang, Gaiping,Liu, Qingtang,Teng, Man,Yang, Jifei,Zhao, Yinli,Wang, Jianhua.

[12]Development of an immunochromatographic lateral flow test strip for detection of beta-adrenergic agonist Clenbuterol residues. Zhang, GP,Wang, XN,Yang, JF,Yang, YY,Xing, GX,Li, QM,Zhao, D,Chai, SJ,Guo, JQ. 2006

[13]Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples. Yang, Xing-dong,Wang, Fang-yu,Zhang, Gai-ping,Yang, Xing-dong,Zeng, Xian-yin,Song, Chun-mei,Wu, Shu-yan,Zhang, Gai-ping.

[14]Development of a one-step strip test for the diagnosis of chicken infectious bursal disease. Zhang, GP,Li, QM,Yan, YY,Guo, JQ,Li, XW,Deng, RG,Xiao, ZJ,Xing, GX,Yang, JF,Zhao, D,Cai, SJ,Zang, WM. 2005

[15]Development of an immunochromatographic strip for the serodiagnosis of Theileria infection in sheep. Lu, Yizhu,Guan, Guiquan,Jiang, Tao,Li, Youquan,Yang, Jifei,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Liu, Zhijie,Yin, Hong. 2015

[16]A Chromatographic Strip for Rapid Semi-quantitative Detection of H5 Subtype Avian Influenza Viruses in Poultry. Li, Ran,Li, Peiwu,Zhang, Wen,Zhang, Qi,Li, Ran,Li, Peiwu,Zhang, Wen,Zhang, Qi,Li, Ran,Li, Peiwu,Guo, Xuebo,Jin, Meilin. 2013

[17]Development of a lateral-flow immunochromatographic test device for the rapid detection of difloxacin residues. Zhi, Ai-min,Li, Bin-bin,Liu, Qing-tang,Hu, Xiao-fei,Zhao, Dong,Hou, Yu-ze,Deng, Rui-guang,Zhang, Gai-ping,Li, Bin-bin,Chai, Shu-Jun. 2010

[18]A rapid one-step immunochromatographic test strip for rabies detection using canine serum samples. Tao, C.,Li, G.,Tao, C..

[19]Rapid detection of aflatoxin B-1 on membrane by dot-immunogold filtration assay. Ye, Yang,Mo, Zhenzhen,Cheng, Wei,Yang, Sha,Wang, Xiaohong,Chen, Fusheng,Wang, Xiaohong,Chen, Fusheng,Chen, Fusheng,Zhou, Youxiang.

[20]Colloidal gold-McAb probe-based rapid immunoassay strip for simultaneous detection of fumonisins in maize. Yao, Jingjing,Sun, Yaning,Li, Qingmei,Wang, Fangyu,Teng, Man,Yang, Yanyan,Deng, Ruiguang,Hu, Xiaofei.

作者其他论文 更多>>

微信小程序