Evaluation of an attenuated vaccine candidate based on the genotype C of bovine parainfluenza virus type 3 in albino guinea pigs

文献类型： 外文期刊

第一作者： Ma Lei

作者： Ma Lei;Zhu Yuan-mao;Yang Ting;Xue Fei

作者机构：

关键词： bovine parainfluenza virus type 3;attenuated vaccine;genotype C;guinea pig

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2017 年 16 卷 9 期

页码：

收录情况： SCI

摘要： Bovine parainfluenza virus type 3 (BPIV3) is considered as one of the most important respiratory tract pathogens of both young and adult cattle, and widespread among cattle in the world. BPIV3 was first reported in China in 2008 and four strains of BPIV3 were isolated from Shandong Province, known as genotype C (BPIV3c). Pathogen investigations had shown that BPIV3c infection was very common among cattle in China. To date, BPIV3 can be classified into genotypes A, B and C based on genetic and phylogenetic analysis. Serological survey also demonstrates that BPIV3 infection is widespread in China, however, there is still no available vaccine for BPIV3 prevention in China nowadays. In the present study, the BPIV3c strain SD0835 was continuously passaged on Madin-Darby bovine kidney (MDBK) cells for hundreds of times, and the pathogenicity of passage 209 was reduced in guinea pigs. The passage 209 of BPIV3c strain SD0835 was used as a live vaccine candidate to immunize the guinea pigs. The vaccination results revealed that two vaccinations could induce excellent serum neutralizing antibody responses as well as proliferation of T lymphocytes. The vaccinated guinea pigs were well protected against challenge with a low passage of BPIV3c strain SD0835. Additionally, the percentages of CD4(+) and CD8(+) T cell subsets of animals in vaccinated group increased after immunization; T cell subsets on day 2 after challenge in both groups decreased, and the decline of CD4(+) and CD8(+) T cell subsets levels of four guinea pigs in vaccinated group was relatively moderate, comparing with that of the control group. These data support further testing of the attenuated virus as an effective candidate vaccine.

分类号：

  • 相关文献

[1]Pathogenesis of a genotype C strain of bovine parainfluenza virus type 3 infection in albino guinea pigs. Shi, Hong-Fei,Zhu, Yuan-Mao,Dong, Xiu-Mei,Cai, Hong,Ma, Lei,Wang, Shu,Yan, Hao,Wang, Xue-Zhi,Xue, Fei.

[2]Studies on the pathogenesis of a Chinese strain of bovine parainfluenza virus type 3 infection in Balb/c mice. Dong, Xiu-Mei,Zhu, Yuan-Mao,Cai, Hong,Lv, Chuang,Gao, Yu-Ran,Yu, Zuo,Xue, Fei,Dong, Xiu-Mei. 2012

[3]Identification of three antigen epitopes on the nucleocapsid protein of the genotype C of bovine parainfluenza virus type 3. Ren, Jian-Le,Zhu, Yuan-Mao,Zhou, Yue-Hui,Lv, Chuang,Yan, Hao,Ma, Lei,Shi, Hong-Fei,Xue, Fei.

[4]Recombinant Bivalent Vaccine against Foot-and-Mouth Disease Virus Serotype O/A Infection in Guinea Pig. Yi, JZ,Liu, MQ,Zhu, CZ,Zhang, Q,Sheng, ZT,Du, QY,Yan, WY,Zheng, ZX.

[5]Development and evaluation of two truncated recombinant NP antigen-based indirect ELISAs for detection of bovine parainfluenza virus type 3 antibodies in cattle. Yang, Yong,Wang, Feng-Xue,Sun, Na,Cao, Li,Zhang, Shu-Qin,Zhu, Hong-Wei,Guo, Li,Cheng, Shi-Peng,Wen, Yong-Jun.

[6]The innate immunity of guinea pigs against highly pathogenic avian influenza virus infection. Zhang, Kun,Xu, Wei wei,Zhang, Zhaowei,Liu, Jing,Sun, Weiyang,Sang, Xiaoyu,Ren, Zhiguang,Yu, Zhijun,Li, Yuanguo,Feng, Na,Wang, Tiecheng,Wang, Hualei,Yang, Songtao,Zhao, Yongkun,Gao, Yuwei,Xia, Xianzhu,Zhang, Kun,Li, Jing,Liu, WenJun,Sun, Lijuan,Zhang, Xuemei,Jiao, Peirong,Liao, Ming,Wilker, Peter R.,Chen, Hualan. 2017

[7]Effects of fluoride and aluminum on expressions of StAR and P450scc of related steroidogenesis in guinea pigs' testis. Dong, Chunguang,Cao, Jinling,Cao, Chunfang,Wang, Jundong,Dong, Chunguang,Han, Yichao,Wu, Shouyan,Wang, Shaolin,Wang, Jundong.

[8]Establishment of reverse transcription loop-mediated isothermal amplification for rapid detection and differentiation of canine distemper virus infected and vaccinated animals. Liu, Da-Fei,Chai, Hong-Liang,Yin, Xiu-Chen,Hua, Yu-Ping,Liu, Da-Fei,Liu, Chun-Guo,Tian, Jin,Zhang, Xiao-Zhan,Yang, Tian-Kuo,Zhang, Hong-Ying,Liu, Ming,Qu, Lian-Dong,Jiang, Yi-Tong.

[9]Amino acid mutations of the infectious clone from Chinese EIAV attenuated vaccine resulted in reversion of virulence. Shen, T,Liang, H,Tong, X,Fan, XJ,He, X,Ma, Y,Xiang, WH,Shen, RX,Zhang, XY,Shao, YM. 2006

[10]Microbial ecology of swine farms and PRRS vaccine vaccination strategies. Zhao, Zhanzhong,Lai, Zhi,Guo, Xin,Yang, Hanchun,Zhao, Zhanzhong,Lai, Zhi,Guo, Xin,Yang, Hanchun,Zhao, Zhanzhong,Qin, Yuming,Wang, Liyong,Peng, Liying,Cai, Xuehui. 2012

[11]The Plasma Load of Inoculated EIAV Vaccine Strain EIAV(FDDV) Was Not Boosted by Immune Suppression. Ma Jian,Jiang Cheng-Gang,Lin Yue-Zhi,Guo Liang,Guo Wei,Kong Xian-Gang,Shen Rong-Xian,Zhou Jian-Hua,Ma Jian,Guo Liang,Shao Yi-Ming,Zhang Xiao-Yan. 2008

[12]Immune Responses to the Attenuated Mycoplasma hyopneumoniae 168 Strain Vaccine by Intrapulmonic Immunization in Piglets. Feng Zhi-xin,Shao Guo-qing,Liu Mao-jun,Wu Xu-su,Zhou Yong-qi,Gan Yuan. 2010

作者其他论文 更多>>

微信小程序