AAC as a Potential Target Gene to Control Verticillium dahliae

文献类型： 外文期刊

第一作者： Su, Xiaofeng

作者： Su, Xiaofeng;Rehman, Latifur;Guo, Huiming;Li, Xiaokang;Zhang, Rui;Cheng, Hongmei

作者机构：

关键词： Verticillium dahliae;VdAAC;RNAi;growth;virulence

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

ISSN： 2073-4425

年卷期： 2017 年 8 卷 1 期

页码：

收录情况： SCI

摘要： Verticillium dahliae invades the roots of host plants and causes vascular wilt, which seriously diminishes the yield of cotton and other important crops. The protein AAC (ADP, ATP carrier) is responsible for transferring ATP from the mitochondria into the cytoplasm. When V. dahliae protoplasts were transformed with short interfering RNAs (siRNAs) targeting the VdAAC gene, fungal growth and sporulation were significantly inhibited. To further confirm a role for VdAAC in fungal development, we generated knockout mutants (Delta VdACC). Compared with wild-type V. dahliae (Vd wt), Delta VdAAC was impaired in germination and virulence; these impairments were rescued in the complementary strains (Delta VdAAC-C). Moreover, when an RNAi construct of VdAAC under the control of the 35S promoter was used to transform Nicotiana benthamiana, the expression of VdAAC was downregulated in the transgenic seedlings, and they had elevated resistance against V. dahliae. The results of this study suggest that VdAAC contributes to fungal development, virulence and is a promising candidate gene to control V. dahliae. In addition, RNAi is a highly efficient way to silence fungal genes and provides a novel strategy to improve disease resistance in plants.

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