Development and utilization of a new chemically-induced soybean library with a high mutation density

文献类型： 外文期刊

第一作者： Li, Zhongfeng

作者： Li, Zhongfeng;Jiang, Lingxue;Ma, Yansong;Wei, Zhongyan;Hong, Huilong;Liu, Zhangxiong;Lei, Jinhui;Liu, Ying;Guan, Rongxia;Guo, Yong;Jin, Longguo;Zhang, Lijuan;Li, Yinghui;Ren, Yulong;He, Wei;Liu, Ming;Htwe, Nang Myint Phyu Sin;Liu, Lin;Guo, Bingfu;Song, Jian;Tan, Bing;Liu, Guifeng;Li, Maiquan;Zhang, Xianli;Liu, Bo;Shi, Xuehui;Han, Sining;Hua, Sunan;Zhou, Fulai;Yu, Lili;Li, Yanfei;Wang, Shuang;Wang, Jun;Chang, Ruzhen;Qiu, Lijuan;Ma, Yansong;Wang, Jun

作者机构：

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2017 年 59 卷 1 期

页码：

收录情况： SCI

摘要： Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate (EMS)-induced soybean (Glycine max) population, consisting of 21,600 independent M-2 lines, was developed. Over 1,000 M-4(5) families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll (Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to MinnGold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene (ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.

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