Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis

文献类型： 外文期刊

第一作者： Li, Shengben

作者： Li, Shengben;Li, Shengben;Le, Brandon;Li, Shaofang;You, Chenjiang;Yu, Yu;Zhang, Bailong;Liu, Lin;Gao, Lei;Shi, Ting;Zhao, Yonghui;Chen, Xuemei;Ma, Xuan;You, Chenjiang;Liu, Lin;Gao, Lei;Mo, Beixin;Chen, Xuemei;Ma, Xuan;Cao, Xiaofeng;Ma, Xuan;Cao, Xiaofeng;Shi, Ting;Chen, Xuemei

作者机构：

期刊名称：ELIFE （ 影响因子：8.14； 五年影响因子：9.056 ）

ISSN： 2050-084X

年卷期： 2016 年 5 卷

页码：

收录情况： SCI

摘要： Small RNAs are central players in RNA silencing, yet their cytoplasmic compartmentalization and the effects it may have on their activities have not been studied at the genomic scale. Here we report that Arabidopsis microRNAs (miRNAs) and small interfering RNAs (siRNAs) are distinctly partitioned between the endoplasmic reticulum (ER) and cytosol. All miRNAs are associated with membrane-bound polysomes (MBPs) as opposed to polysomes in general. The MBP association is functionally linked to a deeply conserved and tightly regulated activity of miRNAs production of phased siRNAs (phasiRNAs) from select target RNAs. The phasiRNA precursor RNAs, thought to be noncoding, are on MBPs and are occupied by ribosomes in a manner that supports miRNA-triggered phasiRNA production, suggesting that ribosomes on the rough ER impact siRNA biogenesis. This study reveals global patterns of cytoplasmic partitioning of small RNAs and expands the known functions of ribosomes and ER.

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