Global DNA methylation variations after short-term heat shock treatment in cultured microspores of Brassica napus cv. Topas

文献类型： 外文期刊

第一作者： Li, Jun

作者： Li, Jun;Huang, Qian;Zhang, Tianyao;Li, Hao;Chen, Biyun;Xu, Kun;Gao, Guizhen;Li, Feng;Yan, Guixin;Qiao, Jiangwei;Wu, Xiaoming;Sun, Mengxiang;Cai, Yongping

作者机构：

期刊名称：Scientific Reports （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2016 年 6 卷

页码：

收录情况： SCI

摘要： Heat stress can induce the cultured microspores into embryogenesis. In this study, whole genome bisulphite sequencing was employed to study global DNA methylation variations after short-term heat shock (STHS) treatments in cultured microspores of Brassica napus cv. Topas. Our results indicated that treatment on cultured Topas microspores at 32 degrees C for 6 h triggered DNA hypomethylation, particularly in the CG and CHG contexts. And the total number of T32 (Topas 32 degrees C for 6 h) vs. T0 (Topas 0 h) differentially methylated region-related genes (DRGs) was approximately two-fold higher than that of T18 (Topas 18 degrees C for 6 h) vs. T0 DRGs, which suggested that 32 degrees C might be a more intense external stimulus than 18 degrees C resulting in more changes in the DNA methylation status of cultured microspores. Additionally, 32 degrees C treatment for 6 h led to increased CHG differential methylations of transposons (DMTs), which were mainly constituted by overlaps between the hypomethylated differentially methylated regions (hypo-DMRs) and transposon elements (TEs). Further analysis demonstrated that the DRGs and their paralogs exhibited differential methylated/demethylated patterns. To summarize, the present study is the first methylome analysis of cultured microspores in response to STHS and may provide valuable information on the roles of DNA methylation in heat response.

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