Polysaccharide from Sepia esculenta ink and cisplatin inhibit synergistically proliferation and metastasis of triple-negative breast cancer MDA-MB-231 cells

文献类型： 外文期刊

第一作者： Liu, Hua-Zhong

作者： Liu, Hua-Zhong;Xiao, Wei;Shang, Jiang-Hua;Xiao, Wei;Zhang, Da-Yan;Gu, Yi-Peng;Tao, Ye-Xing;Du, Hui

作者机构：

关键词： Chou-Talalay method;Cisplatin;MDA-MB-231 cells;Sepia esculenta ink

期刊名称：IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES （ 影响因子：2.699； 五年影响因子：2.629 ）

ISSN： 2008-3866

年卷期： 2016 年 19 卷 12 期

页码：

收录情况： SCI

摘要： Objective(s): This paper aims to investigate synergistic inhibition of polysaccharide from Sepia esculenta ink (SIP), a newly isolated marine polysaccharide in our laboratory, on breast cancer MDA-MB- 231 cells exposed to cisplatin. Materials and Methods: Cell viability of MDA-MB-231 cells was determined by CCK 8 assay. Median-effect concentration was analyzed using Chou-Talalay method that was also subjected to determine cell inhibition ratio and combined index, as well as interaction between SIP and cisplatin. Proliferation and migration abilities were detected with plate colony formation assay and cell wound scratch assay, respectively. Expression of MMP-2 and MMP-9 proteins was measured with Western blot assay. Results: Data showed that SIP not only suppressed proliferation and migration of MDA-MB-231 cells, and expression of MMP-2 and MMP-9 proteins, also promoted inhibition of cisplatin on proliferation, migration and MMPs expression of MDA-MB-231 cells, which indicates synergy inhibition of drug combination of SIP and cisplatin on breast cancer cells. The median-effect concentrations of cisplatin and SIP were 4.9 and 1659.6 mu g/ml, respectively. Whereas the concentration of combination drug was 158.5 mu g/ml. The data indicated that drug combination can decrease dosages of the two single agents, especially the usual dosage of cisplatin. Conclusion: This research demonstrated that SIP repressed proliferation and metastasis of MDA-MB-231 cells and promoted anticancer effect of cisplatin on the breast cancer cells. The data suggested that SIP is a potential natural drug that can be used as an auxiliary medicine alongside chemotherapy in treating breast cancer.

分类号：

  • 相关文献

[1]Role of gga-miR-221 and gga-miR-222 during Tumour Formation in Chickens Infected by Subgroup J Avian Leukosis Virus. Dai, Zhenkai,Yan, Yiming,Lin, Wencheng,Li, Hongxin,Chen, Feng,Liu, Yang,Chen, Weiguo,Bi, Yingzuo,Xie, Qingmei,Dai, Zhenkai,Yan, Yiming,Lin, Wencheng,Li, Hongxin,Chen, Feng,Liu, Yang,Chen, Weiguo,Bi, Yingzuo,Xie, Qingmei,Ji, Jun,Xie, Qingmei,Li, Hongxin,Chen, Feng,Xie, Qingmei,Chen, Feng,Chen, Weiguo,Bi, Yingzuo,Xie, Qingmei,Chen, Feng,Chen, Weiguo,Bi, Yingzuo,Xie, Qingmei. 2015

[2]Silencing of BAG3 promotes the sensitivity of ovarian cancer cells to cisplatin via inhibition of autophagy. Qiu, Shuang,Jin, Ye,An, Qi,Zheng, Jianhua,Sun, Liang,Weng, Changjiang.

[3]Early responses of the STAT3 pathway to platinum drugs are associated with cisplatin resistance in epithelial ovarian cancer. Sheng, W. J.,Jiang, H.,Zheng, J. H.,Wu, D. L.. 2013

作者其他论文 更多>>

微信小程序