Immunogenicity of porcine circovirus type 2 nucleic acid vaccine containing CpG motif for mice

文献类型： 外文期刊

第一作者： Li, Jun

作者： Li, Jun;Yu, Jiang;Xu, Shaojian;Shi, Jianli;Xu, Shengnan;Wu, Xiaoyan;Peng, Zhe;Zhang, Lingling;Zheng, Shuxuan;Yuan, Xiaoyuan;Cong, Xiaoyan;Sun, Wenbo;Cheng, Kaihui;Du, Yijun;Wu, Jiaqiang;Wang, Jinbao;Fu, Fang

作者机构：

关键词： Porcine circovirus type 2;Nucleic acid vaccine;Immunogenicity;CpG motifs;Mice

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2016 年 13 卷

页码：

收录情况： SCI

摘要： Background: This study aimed at reseaching the immune effect of porcine circovirus type 2 (PCV2) DNA vaccine containing CpG motif on mice. Methods: A total of 40 6-week-old female BALB/c mice were randomly divided into four groups which were immunized by 18CpG-pVAX1-ORF2, pVAX1-ORF2, pVAX1 and PBS, respectively, and immunized again 2 weeks later. All mice were challenged with 0.2 mL PCV2 cells virulent strain SD (10(6.0) TCID50/mL) after 4 weeks. Average daily gain, blood antibody levels, microscopic changes and viremia were detected to estimate the effect of DNA vaccine. Results and Discussion: The results showed that compared to those of the control mice, groups immunized with pVAX1-ORF2 and 18CpG-pVAX1-ORF2 could induce PCV2-specific antibodies. The PCV2-specific antibodies level of 18 CpG-pVAX1-ORF2 groups was higher significantly than other groups and decreased slowly along with time. There was no distinct pathological damage and viremia occurring in mice that inoculated with CpG motif DNA vaccines. The results demonstrated that the DNA vaccine containing 18 CpG could build up resistibility immunity and reduce immune organ damage on mice.

分类号：

  • 相关文献

[1]High-level expression and immunogenicity of a porcine circovirus type 2 capsid protein through codon optimization in Pichia pastoris. Tu, Yabin,Wang, Yanqun,Wang, Gang,Wu, Jianan,Liu, Yonggang,Wang, Shujie,Jiang, Chenggang,Cai, Xuehui.

[2]Capsid proteins from PCV2a genotype confer greater protection against a PCV2b strain than those from PCV2b genotype in pigs: evidence for PCV2b strains becoming more predominant than PCV2a strains from 2000 to 2010s. Huang, Liping,Wang, Yiping,Wei, Yanwu,Chen, Dongjie,Liu, Dan,Du, Wenjuan,Xia, Deli,Wu, Hongli,Feng, Li,Liu, Changming.

[3]Recombinant outer membrane protein A induces a protective immune response against Escherichia coli infection in mice. Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Zhang, Yong,Wang, Jianhua,Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Zhang, Yong,Wang, Jianhua.

[4]Effects of CpG ODN on CD4(+) and CD8(+) T subpopulations in the immune response to porcine reproductive and respiratory syndrome killed virus vaccine. Zhang, LH,Tian, XS,Guo, Y,Zhou, FZ. 2006

[5]Effect of transgenic expression of porcine interleukin-6 gene and CpG sequences on immune responses of newborn piglets inoculated with Pseudorabies attenuated vaccine. Zhang, LH,Tian, XS,Guo, Y,Zhou, FZ,Meng, MJ. 2006

[6]Nuclear localization signal regulates porcine circovirus type 2 capsid protein nuclear export through phosphorylation. Hou, Qiang,Hou, Shaohua,Jia, Hong,Xin, Ting,Jiang, Yitong,Guo, Xiaoyu,Zhu, Hongfei,Chen, Qing. 2018

[7]Co-existence of multiple strains of porcine circovirus type 2 in the same pig from China. Zhai, Shao-Lun,Chen, Sheng-Nan,Wei, Wen-Kang,Zhai, Shao-Lun,Wei, Zu-Zhang,Zhang, Jian-Wu,Huang, Lv,Lin, Tao,Yuan, Shi-Shan,Long, Jin-Xue,Zhai, Shao-Lun,Chen, Sheng-Nan,Yue, Cheng,Ran, Duo-Liang. 2011

[8]Porcine circovirus type 2 in China: an update on and insights to its prevalence and control. Zhai, Shao-Lun,Sun, Bei-Bei,Deng, Su-Fang,Hu, Jun,Luo, Man-Lin,Zhai, Shao-Lun,Xu, Zhi-Hong,Lv, Dian-Hong,Wen, Xiao-Hui,Yuan, Jie,Wei, Wen-Kang,Chen, Sheng-Nan,Tang, Man-Hua,Wang, Feng-Guo,Li, Xiao-Jing. 2014

[9]Construction and characterization of porcine circovirus type 2 carrying a genetic marker strain. Liu, Changming,Wei, Yanwu,Zhang, Caofan,Lu, Yueha,Kong, Xiangang. 2007

[10]Three new emerging subgroups of torque teno sus viruses (TTSuVs) and co-infection of TTSuVs with porcine circovirus type 2 in China. Liu, Jianbo,Guo, Longjun,Zhang, Long,Wei, Yanwu,Huang, Liping,Wu, Hongli,Liu, Changming. 2013

[11]Synergistic pathogenicity in sequential coinfection with Mycoplasma hyorhinis and porcine circovirus type 2. Chen, Dongjie,Wei, Yanwu,Huang, Liping,Wang, Yiping,Sun, Jianhui,Du, Wenjuan,Wu, Hongli,Liu, Changming.

[12]Molecular detection and genome characterization of porcine circovirus type 2 in rats captured on commercial swine farms. Zhai, Shao-Lun,Wen, Xiao-Hui,Lv, Dian-Hong,Wei, Wen-Kang,Zhai, Shao-Lun,Wen, Xiao-Hui,Lv, Dian-Hong,Wei, Wen-Kang,Zhai, Shao-Lun,Wen, Xiao-Hui,Lv, Dian-Hong,Wei, Wen-Kang,Chen, Sheng-Nan,Liu, Wei,Chen, Rui-Ai,Li, Xiao-Peng,Deng, Su-Fang,Luo, Man-Lin,Chen, Rui-Ai.

[13]Construction and biological characterisation of recombinant porcine circovirus type 2 expressing the V5 epitope tag. Huang, Liping,Lu, Yuehua,Wei, Yanwu,Guo, Longjun,Wu, Hongli,Zhang, Feiyan,Fu, Yujie,Liu, Changming. 2011

[14]C-terminal heat shock protein 70 of Mycobacterium tuberculosis as a molecular adjuvant for DNA vaccination with the porcine circovirus type 2 ORF2 (capsid) gene in mice. Fu, Fang,Tian, Huabin,Li, Xuesong,Lang, Yuekun,Liu, Siguo,Li, Haizhong,Wang, Wei,Li, Xi,Chen, Xin,Li, Xuesong,Tong, Guangzhi. 2013

[15]Mutant Rep protein of the porcine circovirus type 2 N-glycosylation:23-25aa, 256-258aa mutation reduced virus replication but 286-288aa mutation enhanced virus replication in PK-15 cells. Shi, Jianli,Peng, Zhe,Xu, Shaojian,Cong, Xiaoyan,Yuan, Xiaoyuan,Yu, Jiang,Wu, Jiaqiang,Sun, Wenbo,Du, Yijun,Li, Jun,Wang, Jinbao,Fu, Fang,Xu, Shengnan.

[16]Effect of different selenium sources and levels on porcine circovirus type 2 replication in vitro. Pan, Qunxing,Huang, Kehe,Lu, Fuzeng,Pan, Qunxing,He, Kongwang. 2008

[17]Nanoparticle orientationally displayed antigen epitopes improve neutralizing antibody level in a model of porcine circovirus type 2. Ding, Peiyang,Li, Yafei,Zhou, Enmin,Zhang, Gaiping,Ding, Peiyang,Zhang, Teng,Li, Yafei,Teng, Man,Sun, Yaning,Liu, Xiao,Chai, Shujun,Jin, Qianyue,Zhang, Gaiping,Zhang, Teng,Liu, Xiao,Zhang, Gaiping,Jin, Qianyue,Zhang, Gaiping. 2017

[18]Development of a blocking ELISA for detection of serum neutralizing antibodies against porcine circovirus type 2. Huang, Liping,Lu, Yuehua,Wei, Yanwu,Guo, Longjun,Liu, Changming. 2011

[19]Secreted Expression of the Cap Gene of Porcine Circovirus Type 2 in Classical Swine Fever Virus C-Strain: Potential of C-Strain Used as a Vaccine Vector. Zhang, Lingkai,Li, Yongfeng,Xie, Libao,Wang, Xiao,Sun, Yuan,Qiu, Hua-Ji,Gao, Xulei. 2017

[20]Immunogenicity and immunoprotection of porcine circovirus type 2 (PCV2) Cap protein displayed by Lactococcus lactis. Li, Peng-cheng,Qiao, Xu-wen,Zheng, Qi-sheng,Hou, Ji-bo.

作者其他论文 更多>>

微信小程序