文献类型： 外文期刊
第一作者： Suttiprapa, Sutas
作者： Suttiprapa, Sutas;Rinaldi, Gabriel;Mann, Victoria H.;Dubrovsky, Larisa;Yan, Hong-bin;Pushkarsky, Tatiana;Lordanskiy, Sergey;Bukrinsky, Michael I.;Brindley, Paul J.;Suttiprapa, Sutas;Rinaldi, Gabriel;Mann, Victoria H.;Dubrovsky, Larisa;Yan, Hong-bin;Pushkarsky, Tatiana;Lordanskiy, Sergey;Bukrinsky, Michael I.;Brindley, Paul J.;Suttiprapa, Sutas;Suttiprapa, Sutas;Rinaldi, Gabriel;Tsai, Isheng J.;Holroyd, Nancy;Huckvale, Thomas;Durrant, Caroline;Protasio, Anna V.;Berriman, Matthew;Tsai, Isheng J.;Yan, Hong-bin;Lordanskiy, Sergey
作者机构：
期刊名称：PLOS PATHOGENS （ 影响因子：6.823； 五年影响因子：7.455 ）
ISSN： 1553-7366
年卷期： 2016 年 12 卷 10 期
页码：
收录情况： SCI
摘要： Schistosomiasis is the most important helminthic disease of humanity in terms of morbidity and mortality. Facile manipulation of schistosomes using lentiviruses would enable advances in functional genomics in these and related neglected tropical diseases pathogens including tapeworms, and including their non-dividing cells. Such approaches have hitherto been unavailable. Blood stream forms of the human blood fluke, Schistosoma mansoni, the causative agent of the hepatointestinal schistosomiasis, were infected with the human HIV-1 isolate NL4-3 pseudotyped with vesicular stomatitis virus glycoprotein. The appearance of strong stop and positive strand cDNAs indicated that virions fused to schistosome cells, the nucleocapsid internalized and the RNA genome reverse transcribed. Anchored PCR analysis, sequencing HIV-1-specific anchored Illumina libraries and Whole Genome Sequencing (WGS) of schistosomes confirmed chromosomal integration; >8,000 integrations were mapped, distributed throughout the eight pairs of chromosomes including the sex chromosomes. The rate of integrations in the genome exceeded five per 1,000 kb and HIV-1 integrated into protein-encoding loci and elsewhere with integration bias dissimilar to that of human T cells. We estimated similar to 2,100 integrations per schistosomulum based on WGS, i.e. about two or three events per cell, comparable to integration rates in human cells. Accomplishment in schistosomes of post-entry processes essential for HIV-1replication, including integrase-catalyzed integration, was remarkable given the phylogenetic distance between schistosomes and primates, the natural hosts of the genus Lentivirus. These enigmatic findings revealed that HIV-1 was active within cells of S. mansoni, and provided the first demonstration that HIV-1 can integrate into the genome of an invertebrate.
分类号：
- 相关文献
作者其他论文 更多>>
-
Spatiotemporal Transcriptomic Profiling Reveals the Dynamic Immunological Landscape of Alveolar Echinococcosis
作者：Ou, Zhihua;Ren, Peidi;He, Fan;Chen, Jialing;Tan, Qiming;Chen, Qi;Zhang, Qian;Ou, Zhihua;Ren, Peidi;He, Fan;Chen, Jialing;Cai, Huimin;Li, Jiandong;Tan, Qiming;Chen, Qi;He, Xiuju;Zhao, Yanping;Zhang, Qian;Liang, Yingan;Li, Junhua;Li, Li;Zhou, Ting-Ting;Wu, Yao-Dong;Li, Xiu-Rong;Li, Wenhui;Zhang, Nian-Zhang;Chen, Wei-Gang;Sun, Jiwen;Wu, Yan-Tao;You, Jie;Tian, Xue-Qi;Fu, Bao-Quan;Cai, Xue-Peng;Jia, Wan-Zhong;Yan, Hong-Bin;Li, Li;Zhou, Ting-Ting;Wu, Yao-Dong;Li, Xiu-Rong;Li, Wenhui;Zhang, Nian-Zhang;Chen, Wei-Gang;Sun, Jiwen;Wu, Yan-Tao;You, Jie;Tian, Xue-Qi;Fu, Bao-Quan;Cai, Xue-Peng;Jia, Wan-Zhong;Yan, Hong-Bin;He, Fan;Chen, Jialing;Chen, Qi;Zhang, Qian;Cai, Huimin;Li, Jiandong;He, Xiuju;Zhao, Yanping;Liao, Sha;Yang, Huanming;Wang, Jian;Jin, Xin;Xu, Xun;Han, Xiumin;Tan, Qiming;Liang, Yingan;Hu, Guohai;Wang, Jian;Chen, Ao;Chen, Ao;Jin, Xin;Jin, Xin;Xu, Xun;Li, Junhua
关键词：
Echinococcus multilocularis ; macrophages; neutrophils; single-cell RNA sequencing; spatial transcriptomics -
Epidemiology, molecular diversity and zoonotic implications of Echinococcus granulosus sensu stricto (G1) in cattle across Nigeria: Evidence of broader transmission dynamics
作者：Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Morenikeji, Olajumoke Abimbola;Odaibo, Georgina Nijideka;Onoja, Bernard Anyebe;Akanbi, Ibikunle Mark;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong
关键词：Echinococcus granulosus sensu lato; Cystic echinococcosis; Nigeria; Genetic diversity; Molecular epidemiology; One health
-
A new intermediate host of Echinococcus shiquicus in Qinghai-Tibet Plateau, China
作者：Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Hou, Xue-Yi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Guo, Min-Kuan;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Shumuye, Nigus Abebe;Gao, Sheng-Zhi;Hou, Xue-Yi;Zhonggecao, Tao;Zhaxilamao, Bao-Quan;Gazangzhuoma, Hong;Doukao, Hong-Bin;Zhang, Fu-Heng;Guo, Min-Kuan;Li, Wen-Dong;Yang, Tao;Fu, Bao-Quan;Yin, Hong;Jia, Wan-Zhong
关键词：Echinococcus shiquicus; Plateau zokor; Intermediate host; Qinghai-Tibet Plateau; Genetic diversity
-
Molecular detection and genetic diversity of Anaplasma and Ehrlichia species in livestock and dogs in Pakistan
作者：Alvi, Mughees Aizaz;Yan, Hong-Bin;Jia, Wan-Zhong;Li, Li;Alvi, Mughees Aizaz;Yan, Hong-Bin;Jia, Wan-Zhong;Li, Li;Alvi, Mughees Aizaz;Javaid, Talha;Yameen, Abu Bakar;Asghar, Fakiha;Ali, Aliza;Saqib, Muhammad;Zaheer, Iqra;Fadladdin, Yousef Abdal Jalil
关键词：Tick-borne diseases; Molecular epidemiology; Genetic diversity; Anaplasmosis
-
High Genetic Variability in Full-length cox2 and nad6 Genes of Echinococcus granulosus sensu stricto and Echinococcus ortleppi Recovered from Cattle
作者：Malik, Muhammad Abdullah;Subhani, Muhammad Irshad;Alvi, Mughees Aizaz;Saqib, Muhammad;Alvi, Mughees Aizaz;Li, Li;Liu, You-Yu;Fu, Bao-Quan;Yan, Hong-Bin;Jia, Wan-Zhong;Alvi, Mughees Aizaz;Li, Li;Liu, You-Yu;Fu, Bao-Quan;Yan, Hong-Bin;Jia, Wan-Zhong;Wakid, Majed H.;Wakid, Majed H.;Alkhaldi, Abdulsalam A. M.;Khan, Muzaffar Ali
关键词：Echinococcus granulosus; Echinococcus ortleppi; cox2; nad6; Genetic variation; Population structure
-
Genetic variation and population structure of Taenia multiceps (Coenurus cerebralis) based on mitochondrial cox1 gene: A comprehensive global analysis
作者：Ul Haq, Shahbaz;Gao, Fenfei;Malik, Muhammad Abdullah;Qamar, Warda;Sajid, Muhammad Sohail;Alshammari, Ayed;Yameen, Abu Bakar;Alvi, Mughees Aizaz;Saqib, Muhammad;Wakid, Majed H.;Wakid, Majed H.;Alvi, Mughees Aizaz;Li, Li;Fu, Bao-Quan;Yan, Hong-Bin;Jia, Wan-Zhong;Alvi, Mughees Aizaz;Li, Li;Fu, Bao-Quan;Yan, Hong-Bin;Jia, Wan-Zhong;Kabli, Abdulbaset Mohammad
关键词：cox 1; Genetic diversity; Haplotypes; Sheep; Goat; Dog
-
Molecular Characterization of Hydatigera taeniaeformis Recovered from Rats: An Update from Pakistan
作者：Alvi, Mughees Aizaz;Li, Li;Fu, Bao-Quan;Yan, Hong-Bin;Jia, Wan-Zhong;Alvi, Mughees Aizaz;Ali, Rana Muhammad Athar;Rashid, Imaad;Saqib, Muhammad;Shafeeq, Muhammad;Ashfaq, Khurram;Alshammari, Ayed;Qamar, Warda;Askar, Hussam;Abdelsater, Naser;Butt, Asif Ali;Jia, Wan-Zhong
关键词：Hydatigera taeniaeformis; NADH dehydrogenase subunit 1; Phylogeny; Genetic variation; Pakistan
