Cloning, expression and functional analysis of a delta 6-desaturase gene from the silkworm, Bombyx mori L.

文献类型： 外文期刊

第一作者： Yu, Hai-Yan

作者： Yu, Hai-Yan;Zhou, Zhi-Feng;Jia, Jun-Qiang;Gui, Zhong-Zheng;Jia, Jun-Qiang;Gui, Zhong-Zheng

作者机构：

关键词： Bombyx mori;BmD6DES;Cloning;Expression;Functional analysis

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.303； 五年影响因子：1.427 ）

ISSN： 1226-8615

年卷期： 2016 年 19 卷 3 期

页码：

收录情况： SCI

摘要： Delta 6-fatty acid desaturase is a membrane-bound enzyme, which is the rate-limiting factor in the biosynthesis of polyunsaturated fatty acids. In this study, a novel delta 6-desaturase gene was cloned from Bombyx mori (BmD6DES). Sequencing analysis revealed that BmD6DES has an open reading frame of 1357 by that encodes 448 amino acids. Heterologous expression in yeast demonstrated that BmD6DES could synthesize gamma-linolenic acid (GLA, 18:3, Delta(6.9.12)) by utilizing the endogenous substrate linoleic acid (LA, 18:2, Delta(9.12)). We found that BmD6DES transcripts were distributed in almost all B. mori tissues, with high expression levels observed at the 5th instar larval, pupal, and adult moth stages. A functional analysis of BmD6DES was performed by measuring mRNA levels after temperature stress, fungal infection, and RNA interference (RNAi). The results indicated that the highest expression of BmD6DES was observed at low temperatures (0 degrees C) and 6 h to 36 h after fungal infection. qPCR analysis demonstrated that BmD6DES mRNA levels in pupa after BmD6DES RNAi treatment were significantly reduced from 12 h to 72 h compared to those in the control group. Our findings suggest that BmD6DES not only induces the formation of the third carbon-carbon double bond in the LA carbon chain, but also leads to sensitivity to low-temperature stress and fungal infection. These results imply that BmD6DES is a key gene in the gamma-linolenic acid pathway during B. mori development. (C) 2016 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.

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