Functional characterization of X-prolyl aminopeptidase from Toxoplasma gondii

文献类型： 外文期刊

第一作者： Yang, Mingfa

作者： Yang, Mingfa;Song, Mingxin;Yang, Mingfa;Zheng, Jun;Jia, Honglin

作者机构：

关键词： Toxoplasma gondii;Toxoplasmosis;X-prolyl aminopeptidase;enzymatic activity;growth

期刊名称：PARASITOLOGY （ 影响因子：3.234； 五年影响因子：3.118 ）

ISSN： 0031-1820

年卷期： 2016 年 143 卷 11 期

页码：

收录情况： SCI

摘要： In the present study, a recombinant aminopeptidase P (rTgAPP) from Toxoplasma gondii was expressed in Escherichia coli to evaluate its enzyme parameters. The rTgAPP showed strong activity against a synthetic substrate for aminopeptidase P at pH 8.0 with a K-m value of 0.255 mu M and a k(cat) value of 35.6 s(-1). The overall catalytic efficiency (k(cat)/K-m) of the rTgAPP was 139.6 x 10(5) M-1 s(-1). The activity of rTgAPP was enhanced by the addition of divalent cations and inhibited by bestatin. Deletion of TgAPP gene in the parasite through a CRISPR/Cas9 system resulted in inhibition of growth indicating the importance of TgAPP. Thus our findings reveal that TgAPP is an active enzyme in T. gondii and provide an insight into the function of TgAPP.

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