Quantitative analysis of diet structure by real-time PCR, reveals different feeding patterns by two dominant grasshopper species

文献类型： 外文期刊

第一作者： Wu, Huihui

作者： Wu, Huihui;Qin, Xinghu;Ma, Jingchuan;Tu, Xiongbing;Cao, Guangchun;Wang, Guangjun;Nong, Xiangqun;Zhang, Zehua;Huang, Xunbing;Wu, Huihui;Qin, Xinghu;Ma, Jingchuan;Tu, Xiongbing;Cao, Guangchun;Wang, Guangjun;Nong, Xiangqun;Zhang, Zehua;McNeill, Mark Richard

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期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2016 年 6 卷

页码：

收录情况： SCI

摘要： Studies on grasshopper diets have historically employed a range of methodologies, each with certain advantages and disadvantages. For example, some methodologies are qualitative instead of quantitative. Others require long experimental periods or examine population-level effects, only. In this study, we used real-time PCR to examine diets of individual grasshoppers. The method has the advantage of being both fast and quantitative. Using two grasshopper species, Oedaleus asiaticus and Dasyhippus barbipes, we designed ITS primer sequences for their three main host plants, Stipa krylovii, Leymus chinensis and Cleistogenes squarrosa and used real-time PCR method to test diet structure both qualitatively and quantitatively. The lowest detection efficiency of the three grass species was similar to 80% with a strong correlation between actual and PCR-measured food intake. We found that Oedaleus asiaticus maintained an unchanged diet structure across grasslands with different grass communities. By comparison, Dasyhippus barbipes changed its diet structure. These results revealed why O. asiaticus distribution is mainly confined to Stipa-dominated grassland, and D. barbipes is more widely distributed across Inner Mongolia. Overall, real-time PCR was shown to be a useful tool for investigating grasshopper diets, which in turn offers some insight into grasshopper distributions and improved pest management.

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