The response of glutathione peroxidase 1 and glutathione peroxidase 7 under different oxidative stresses in black tiger shrimp, Penaeus monodon

文献类型： 外文期刊

第一作者： Liu, Wei

作者： Liu, Wei;Zhao, Chao;Wang, Pengfei;Qiu, Lihua;Liu, Wei;Lin, Heizhao;Qiu, Lihua;Wang, Shu

作者机构：

关键词： Antioxidant activity;Glutathione peroxidase;mRNA expression;Oxidative stresses;Penaeus monodon

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

ISSN： 1096-4959

年卷期： 2018 年 217 卷

页码：

收录情况： SCI

摘要： Glutathione peroxidase (GPx) is part of the enzymatic antioxidant system that can eliminate the peroxides produced as effect of reactions of molecules with reactive oxygen species (ROS). We cloned two different GPx genes from Penaeus monodon (black tiger shrimp). Bioinformatics sequence analysis showed that PmGPx1 and PmGPx7 encoded polypeptides of 207 and 208 amino acids, respectively. PmGPx1 has a selenocysteine residue that is encoded by an opal codon 187TGA189. The PmGPx1 and PmGPx7 expression profiles were detected in the gills and hepatopancreas of the shrimps under osmotic stress, heavy metal exposure and bacterial infection, and the results suggested that PmGPx1 and PmGPx7 are involved in the responses to these stimuli. The recombinant PmGPx1 and PmGPx7 protein was expressed and purified through affinity chromatography and was refolded successfully using ion-exchange chromatography, which were used to measure antioxidant activity, and the results revealed that the rPmGPxl and rPmGPx7 protein could catalyze the redox reaction between GSH and H2O2. This study provides useful information to help further understand the functional mechanism of the GPx family in the immunity of P. monodon.

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