Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara

文献类型： 外文期刊

第一作者： Liu, Xiao-Feng

作者： Liu, Xiao-Feng;Wu, Ya-Hong;Wang, Na;Li, Yang-Zhen;Zhang, Nian-Wei;Chen, Song-Lin;Liu, Xiao-Feng;Chen, Song-Lin;Liu, Xiao-Feng;Wei, Shi-Na;Li, Peng-Fei;Qin, Qi-Wei

作者机构：

关键词： Kidney cell line;Epinephelus moara;Transfection;SGIV;NNV

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN： 0920-1742

年卷期： 2018 年 44 卷 1 期

页码：

收录情况： SCI

摘要： A novel cell line, Epinephelus moara kidney cell line (EMK), was established from kidneys of kelp grouper E. moara. Cells were cultured at 24 A degrees C in Leibovitz's L-15 medium (L15) supplemented with antibiotics, basic fibroblast growth factor (bFGF), foetal bovine serum (FBS) and 2-mercaptoethanol (2-ME). EMK cells, fibroblastic in morphology, proliferated to 100% confluency in 3-4 days and were subcultured for over 50 passages. The cells could grow from 18 to 30 A degrees C, with optimal growth at 24 A degrees C. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 42. Green fluorescent signals could be observed in EMK cells when the cells were transfected with pEGFP-N3 plasmid. Moreover, a significant cytopathic effect (CPE) was observed in the cells after infection with Singapore grouper iridovirus (SGIV) or nervous necrosis virus (NNV), and viral replication was confirmed by quantitative real-time PCR (qPCR). These results suggested the potential of the EMK cell line for studies of transgene and pathogenesis of SGIV and NNV.

分类号：

  • 相关文献

[1]Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara. Liu, X. F.,Wu, Y. H.,Wang, N.,Li, Y. Z.,Zhang, N. W.,Chen, S. L.,Liu, X. F.,Chen, S. L.,Liu, X. F.,Wei, S. N.,Li, P. F.,Qin, Q. W.. 2018

[2]Establishment, characterization and virus susceptibility of a kidney-derived cell line from southern flounder, Paralichthys lethostigma Jordan & Gilbert. Wang, N.,Wang, X-L,Sha, Z-X,Tian, Y-S,Chen, S-L,Chen, S-L. 2011

[3]Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus). Wang, N.,Wang, X. L.,Sha, Z. X.,Tian, Y. S.,Chen, S. L..

[4]Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection. Tu, Jiagang,Chen, Wenjie,Zhao, Lijuan,Lan, Jiangfeng,Lin, Li,Fu, Xiaozhe,Lin, Qiang,Chang, Ouqin,Li, Ningqiu. 2016

[5]Diagnosis and ORF Gene Sequencing Analysis of The Nervous Necrosis Virus (NNV) Isolated from Cultured Pearl Gentian Grouper, Epinephelus lanceolatus x Epinephelus fuscoguttatus, in China. Ma, Rong-Rong,Wang, Yin-Geng,Liao, Mei-Jie,Zhang, Zheng,Rong, Xiao-Jun,Li, Bin,Ma, Rong-Rong,Yang, Xian-Le. 2014

[6]Cetyltriethylammonium bromide stimulating transcription of Bombyx mori nucleopolyhedrovirus gp64 gene promoter mediated by viral factors. Zhou, YJ,Yi, YZ,Zhang, ZF,He, JL,Zhang, YX.

[7]Effects of cetyltriethylammonium bromide on the replication of Bombyx mori nucleopolyhedrovirus. Zhou, YJ,Zhang, ZF,He, JL,Zhang, YX.

[8]Immune response in guinea pigs vaccinated with DNA vaccine of foot-and-mouth disease virus O/China99. Guo, HC,Liu, ZX,Sun, SQ,Bao, HF,Chen, YL,Liu, XT,Xie, QG.

[9]Establishment and characterization of a fish-cell line from the brain of Japanese flounder Paralichthys olivaceus. Zheng, Y.,Peng, L. M.,You, F.,Zou, Y. X.,Zhang, P. J.,Peng, L. M.,Chen, S. L..

[10]A Preliminary Study on Stable Transfection of EGFP in Buffalo Cumulus Cells. Shang, Jiang-Hua,Yang, Chun-Yan,Qin, Jing,Zheng, Hai-Ying,Huang, Fen-Xiang,Wang, Jian,Liu, Hua-Zhong,Liu, Hua-Zhong. 2013

[11]Promoter activities in the baculovirus envelope glycoprotein gp64 gene. Zhou, YJ,Yi, YZ,Zhang, ZF,He, JL,Zhang, YX,Wu, XF. 2003

[12]The technology of large-scale pharmaceutical plasmid purification by cetyltrimethylammonium bromide and Tritonx-114. Hou, S. H.,Zhu, H. F.,Zhang, Q.,Yuan, W. F.,Sun, H. C.. 2010

[13]Optimization of the Transfection Efficiency of Buffalo Fetal Fibroblasts Cells Using Liposome. Deng, Tingxian,Pang, Chunying,Zhang, Yingyin,Yang, Bingzhuang,Liang, Xianwei. 2013

作者其他论文 更多>>

微信小程序