Identification, structural characterization, and expression analysis of toll-like receptors 2 and 3 from gibel carp (Carassius auratus gibelio)

文献类型： 外文期刊

第一作者： Fan, Yuding

作者： Fan, Yuding;Zhou, Yong;Zeng, Lingbing;Jiang, Nan;Liu, Wenzhi;Zhao, Jianqing;Zhong, Qiwang

作者机构：

关键词： Toll-like receptor;TLR2;TLR3;Gibel carp;Gene structure;Expression;Immune response;CyHV-2

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2018 年 72 卷

页码：

收录情况： SCI

摘要： Toll-like receptors (TLRs) are important components of innate immunity. TLRs recognize pathogen-associated molecular patterns (PAMPs) and initiate downstream signaling pathways in response. In present study, we report the identification of two TLRs from gibel carp (Carassius auratus gibelio), TLR2 and TLR3 (designated CagTLR2 and CagTLR3, respectively). We report on the genomic structures and mRNA expression patterns of CagTLR2 and CagTLR3. Five exons and four introns were identified from the genomic DNA sequence of CagTLR3 (4749 bp in total length); this genomic organization is similar to that of TLR3 in zebrafish and human. However, only one intron was identified from the CagTLR2 genomic locus (3166 bp in total length); this unique genomic organization of CagTLR2 is different from that of TLR2 in fish and humans. The cDNAs of CagTLR2 and CagTLR3 encoded 791 and 904 amino acid residues, respectively. CagTLR2 and CagTLR3 contained two distinct structural/functional motifs of the TLR family: a leucine-rich repeat (LRR) domain in the extracellular portion and a toll/interleukin-1 receptor (TIR) domain in the intracellular portion. The positions of critical amino acid residues involed in PANT recognition and signaling pathway transduction in mammalian TLRs were conserved in CagTLR2 and CagTLR3. Phylogenetic analysis revealed a closer clustering of CagTLR2 and CagTLR3 with TLRs from freshwater fish than with marine fish species. In healthy gibel carp, transcripts of these genes were detected in all examined tissues, and high expression levels of CagTLR2 and CagTLR3 were observed in liver and brain, respectively. Following injection with CyHV-2, expression levels of CagTLR2 and CagTLR3 were significantly upregulated in the spleens of gibel carp after three days, and CagTLR3 transcript levels were rapidly increased in head kidney after 12 h. These results suggest that CagTLR2 and CagTLR3 are functionally involved in the induction of antiviral immune response.

分类号：

  • 相关文献

[1]Genome-wide characterization of Toll-like receptor gene family in common carp (Cyprinus carpio) and their involvement in host immune response to Aeromonas hydrophila infection. Gong, Yiwen,Feng, Shuaisheng,Li, Shangqi,Zhang, Yan,Zhao, Zixia,Jiang, Yanliang,Gong, Yiwen,Hu, Mou,Jiang, Yanliang,Xu, Peng. 2017

[2]Duplication and Divergence of Leucine-Rich Repeat Receptor-Like Protein Kinase (LRR-RLK) Genes in Basal Angiosperm Amborella trichopoda. Liu, Ping-Li,Mao, Jian-Feng,Liu, Hui,Gao, Shu-Min,Shi, Peng-Hao,Gong, Jun-Qing,Xie, Lu-Lu,Li, Peng-Wei. 2016

[3]Characterization of miRNAs involved in response to poly(I: C) in porcine airway epithelial cells. Wang, L.,Wang, J. K.,Han, L. X.,Zhuo, J. S.,Du, X.,Yang, X. Q.,Wang, L.,Liu, D..

[4]Co-administration of porcine-specific CpG oligodeoxynucleotide enhances the immune responses to pseudorabies attenuated virus vaccine in newborn piglets in vivo. Zhang, LH,Guo, Y,Tian, XS,Zhou, FZ.

[5]Protective immunity in gibel carp, Carassius gibelio of the truncated proteins of cyprinid herpesvirus 2 expressed in Pichia pastoris. Zhou, Yong,Zhang, Linlin,Zeng, Lingbing,Zhou, Yong,Jiang, Nan,Ma, Jie,Fan, Yuding,Zhang, Linlin,Xu, Jin,Zeng, Lingbing.

[6]Toll-Like Receptor 2 Gene Polymorphism and its Relationship with SCS in Dairy Cattle. Zhang, L. P.,Li, H. D.,Li, J. Y.,Gao, X.,Chen, J. B.,Ren, H. Y.,Xu, S. Z.,Gan, Q. F.,Wang, X. P.,Xu, S. Z.,Ma, T. H.. 2009

[7]Recombinant TB10.4 of Mycobacterium bovis induces cytokine production in RAW264.7 macrophages through activation of the MAPK and NF-kappa B pathways via TLR2. Liu, Shuqing,Jia, Hong,Hou, Shaohua,Zhang, Gaimei,Xin, Ting,Yuan, Weifeng,Guo, Xiaoyu,Li, Ming,Wu, Jing,Zhu, Hongfei,Li, Hegang,Li, Hegang,Gao, Xintao. 2014

[8]Haplotype variation of toll-like receptor 3 among pig populations. Yang, X. -Q.,Zhai, C. -Y.,Li, H. -T.,Liu, D.. 2011

[9]Functional characterization of genetic variants in the porcine TLR3 gene. Wang, L.,Chen, Y. C.,Li, H. T.,Yang, X. Q.,Wang, L.,Zhang, D. J.,Liu, D.. 2014

[10]The Transcriptomes of the Crucian Carp Complex (Carassius auratus) Provide Insights into the Distinction between Unisexual Triploids and Sexual Diploids. Li, Chun-Yan,Kuang, You-Yi,Sun, Xiao-Wen,Li, Chun-Yan,Sun, Xiao-Wen,Li, Chun-Yan,Li, Jiong-Tang,Xu, Ru,Zhao, Zi-Xia,Hou, Guang-Yuan,Sun, Xiao-Wen,Li, Chun-Yan,Xu, Ru,Liang, Hong-Wei. 2014

[11]Effects of DL-methionine supplementation on the success of fish meal replacement by plant proteins in practical diets for juvenile gibel carp (Carassius auratus gibelio). Ren, M.,Ge, X.,Xie, J.,Xi, B.,Liang, H.,Yang, H.,Ge, X.,Xie, J.,Xi, B.,He, J.,Yue, Y.,Masagounder, K.. 2017

[12]Effects of repeated handling and air exposure on the immune response and the disease resistance of gibel carp (Carassius auratus gibelio) over winter. Yang, Bingyuan,Tu, Yongqin,Hu, Huihua,Han, Dong,Zhu, Xiaoming,Jin, Junyan,Yang, Yunxia,Xie, Shouqi,Yang, Bingyuan,Tu, Yongqin,Hu, Huihua,Wang, Cuicui,Han, Dong.

[13]Gibel carp Carassius auratus gut microbiota after oral administration of trimethoprim/sulfamethoxazole. Liu, Yuchun,Zhou, Zhigang,Xu, Li,Cao, Yanan,Zhang, Yuting,Yao, Bin,Wu, Ning,Tao, Yu. 2012

[14]Evidence that ghrelin may be associated with the food intake of gibel carp (Carassius auratus gibelio). Zhou, Chaowei,Zheng, Jinfa,Lei, Luo,Zhu, Chengke,Ye, Hua,Wang, Dan,Yang, Minmin,Wu, Jingjing,Zhu, Long,Zeng, Benhe,Yuan, Dengyue,Zhang, Chi.

[15]Co-expression of march5b and tlr7 in large yellow croaker Larimichthys crocea in response to Cryptocaryon irritans infection. Zhang, D. L.,Chen, J.,Wang, Z. Y.,Yu, D. H.,Chen, C..

[16]Identification, characterization and expression analysis of TLR5 in the mucosal tissues of turbot (Scophthalmus maximus L.) following bacterial challenge. Liu, Fengqiao,Fu, Qiang,Gao, Chengbin,Li, Chao,Su, Baofeng,Shang, Mei,Su, Baofeng,Shang, Mei,Tan, Fenghua.

[17]Eimeria tenella heat shock protein 70 enhances protection of recombinant microneme protein MIC2 subunit antigen vaccination against E. tenella challenge. Zhang, Lei,Ma, Liping,Liu, Renqiang,Zhang, Yunfei,Zhang, Shouping,Hu, Chunmei,Song, Meng,Wang, Ming,Cai, Jianping. 2012

[18]Porcine parvovirus infection activates inflammatory cytokine production through Toll-like receptor 9 and NF-kappa B signaling pathways in porcine kidney cells. Zhou, Yong,Jin, Xiao-hui,He, Xiao-xiang,Wei, Zhan-yong,Zhang, Gai-ping,Jing, Ya-xing,Song, Yue,Zheng, Lan-Lan,Wang, Ya-bin,Wei, Zhan-yong,Zhang, Gai-ping.

[19]Inactivated vaccine with adjuvants consisting of pattern recognition receptor agonists confers protection against avian influenza viruses in chickens. Tang, Yinghua,Lu, Jihu,Wu, Peipei,Liu, Zhenxing,Tian, Zhen,Zha, Guofei,Chen, Hui,Wang, Qiaochu,Hou, Fengxiang,Hou, Jibo,Tang, Yinghua,Wang, Qiaoxiu,Kang, Sang-Moo.

[20]Genome-wide identification, phylogeny, and expression analysis of pectin methylesterases reveal their major role in cotton fiber development. Weijie Li,Haihong Shang,Qun Ge,Changsong Zou,Juan Cai,Daojie Wang,Senmiao Fan,Zhen Zhang,Xiaoying Deng,Yunna Tan,Weiwu Song,Pengtao Li,Palanga Kibalou Koffi,Muhammad Jamshed,Quanwei Lu,Wankui Gong,Junwen Li,Yuzhen Shi,Tingting Chen,Juwu Gong,Aiying Liu,Youlu Yuan. 2016

作者其他论文 更多>>

微信小程序