Electrophysiological and pharmacological properties of the slowpoke channel in the diamondback moth, Plutella xylostella

文献类型： 外文期刊

第一作者： Huo, Xiaoyi

作者： Huo, Xiaoyi;Liu, Zheming;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhou, Xiaomao;Ma, Haihao;Huo, Xiaoyi;Wang, Yinna;Zhou, Xiaomao;Ma, Haihao;Liu, Zheming;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhou, Xiaomao;Ma, Haihao;Liu, Zheming;Liu, Jia;Zhu, Hang;Zhou, Yong;Zhou, Xiaomao;Ma, Haihao

作者机构：

关键词： Plutella xylostella; Slowpoke channel; Patch clamp; Electrophysiology; Inhibitor

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 0048-3575

年卷期： 2024 年 200 卷

页码：

收录情况： SCI

摘要： The slowpoke channel responds to the intracellular calcium concentration and the depolarization of the cell membrane. It plays an important role in maintaining the resting potential and regulating the homeostasis of neurons, but it can also regulate circadian rhythm, sperm capacitation, ethanol tolerance, and other physiological processes in insects. This renders it a potentially useful target for the development of pest control strategies. There are relatively few studies on the slowpoke channels in lepidopteran pests, and their pharmacological properties are still unclear. So, in this study, the slowpoke gene of Plutella xylostella (Pxslo) was heterologous expressed in HEK293T cells, and the I-V curve of the slowpoke channel was measured by whole cell patch clamp recordings. Results showed that the slowpoke channel could be activated at -20 mV with 150 mu M Ca2+. The subsequent comparison of the electrophysiological characteristics of the alternative splicing site E and G deletions showed that the deletion of the E site enhances the response of the slowpoke channel to depolarization, while the deletion of the G site weakens the response of the slowpoke channel to depolarization. Meanwhile, the nonspecific inhibitors TEA and 4-AP of the Kv channels, and four pesticides were tested and all showed an inhibition effect on the PxSlo channel at 10 or 100 mu M, suggesting that these pesticides also target the slowpoke channel. This study enriches our understanding of the slowpoke channel in Lepidopteran insects and can aid in the development of relevant pest management strategies.

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