Molecular cloning of heat shock protein 60 from Marsupenaeus japonicus and its expression profiles at early developmental stages and response to heat stress

文献类型： 外文期刊

第一作者： Zheng, Jinbin

作者： Zheng, Jinbin;Li, Lijun;Mao, Yong;Su, Yongquan;Wang, Jun;Dong, Hongbiao;Mao, Yong

作者机构：

关键词： development;heat shock protein 60;heat stress;Marsupenaeus japonicus;thermotolerance;water temperature

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

ISSN： 1355-557X

年卷期： 2018 年 49 卷 1 期

页码：

收录情况： SCI

摘要： Water temperature is an essential environmental factor in aquaculture that affects many aspects of organism, and a rise in water temperature stresses aquatic animals. HSP60 is a major component of the chaperone system and plays an important role in stress response. In this study, the full-length cDNA sequence of HSP60 from Marsupenaeus japonicus was cloned for the first time, and the tissue distributions and expression profiles of MjHSP60 at the early developmental stages of M.japonicus and under heat stress were verified by qRT-PCR. The full-length cDNA sequence of MjHSP60 was 2,303bp with the deduced amino acid (AA) sequence of 579 AA. MjHSP60 was expressed in all eight tested tissues of M.japonicus and was mainly expressed in the hepatopancreas under normal condition. MjHSP60 was expressed in all of the early developmental stages examined from the fertilized egg to the post-larval stage, and the expression level of MjHSP60 peaked at zoea II and reached a trough at the transition periods between each two stages (N and M1) and showed a relatively stable expression level at the post-larval stage. The expression level of MjHSP60 was significantly up-regulated in the gills, muscle, heart, hepatopancreas and stomach at 3hr post-heat-stress and showed varied sensitivity to heat stress. The expression profile of MjHSP60 in the hepatopancreas and gill showed a time-dependent manner post-heat-stress and peaked at 3hr post-heat-stress. The present study provided a basis for further studies for elucidating the function of MjHSP60 in the heat stress response and the early developmental stages of M.japonicus.

分类号：

  • 相关文献

[1]The transport of C-14-salicylic acid in heat-stressed young Vitis vinifera plants. Wang, LJ,Huang, WD,Zhan, JC,Yu, FY.

[2]Differential Expression Patterns of Pleurotus ostreatus Catalase Genes during Developmental Stages and under Heat Stress. Wang, Lining,Wu, Xiangli,Gao, Wei,Zhao, Mengran,Zhang, Jinxia,Huang, Chenyang,Wang, Lining,Wu, Xiangli,Gao, Wei,Zhao, Mengran,Zhang, Jinxia,Huang, Chenyang. 2017

[3]Developmental Expression of HSP60 and HSP10 in the Coilia nasus Testis during Upstream Spawning Migration. Fang, Di-An,Zhou, Yan-Feng,Zhang, Min-Ying,Xu, Dong-Po,Liu, Kai,Duan, Jin-Rong,Fang, Di-An,Xu, Dong-Po. 2017

[4]Characterization of the Toxoplasma gondii hsp60 gene sequences from different hosts and geographical locations. Lu, J.,Zhou, D. H.,Chen, J.,Zhang, N. Z.,Wang, R. A.,Zhu, X. Q.,Lu, J.,Wang, R. A.,Weng, Y. B.. 2014

[5]Differential expression of heat shock protein 90, 70, 60 in chicken muscles postmortem and its relationship with meat quality. Zhang, Muhan,Wang, Daoying,Geng, Zhiming,Sun, Chong,Bian, Huan,Xu, Weimin,Zhu, Yongzhi,Li, Pengpeng,Xu, Weimin.

[6]Bioassay evidence for the transmission of WSSV by the harpacticoid copepod Nitocra sp.. Zhang, Jia-Song,Dong, Shuang-Lin,Dong, Yun-Wei,Tian, Xiang-Li,Hou, Chun-Qiang,Zhang, Jia-Song. 2008

[7]Effect of desiccation and resubmersion on the oxidative stress response of the kuruma shrimp Marsupenaeus japonicus. Duan, Yafei,Zhang, Jiasong,Dong, Hongbiao,Wang, Yun,Liu, Qingsong,Li, Hua.

[8]The application of bioflocs technology in high-intensive, zero exchange farming systems of Marsupenaeus japonicus. Zhao, Pei,Huang, Jie,Wang, Xiu-Hua,Song, Xiao-Ling,Yang, Cong-Hai,Zhang, Xu-Guang,Wang, Guo-Cheng,Zhao, Pei,Zhang, Xu-Guang,Wang, Guo-Cheng. 2012

[9]High-resolution genetic linkage mapping, high-temperature tolerance and growth-related quantitative trait locus (QTL) identification in Marsupenaeus japonicus. Lu, Xia,Luan, Sheng,Hu, Long Yang,Kong, Jie,Lu, Xia,Luan, Sheng,Hu, Long Yang,Kong, Jie,Mao, Yong,Zhong, Sheng Ping,Tao, Ye.

[10]Effect of the dietary probiotic Clostridium butyricum on growth, intestine antioxidant capacity and resistance to high temperature stress in kuruma shrimp Marsupenaeus japonicus. Duan, Yafei,Zhang, Yue,Dong, Hongbiao,Wang, Yun,Zhang, Jiasong.

[11]NPR1-dependent salicylic acid signaling is not involved in elevated CO2-induced heat stress tolerance in Arabidopsis thaliana. Li, Xin,Ahammed, Golam Jalal,Li, Xin,Yu, Jingquan,Shi, Kai. 2015

[12]Effects of Carbon Concentrations and Carbon to Nitrogen ratios on Sporulation of Two Biological Control Fungi as Determined by Different Culture Methods. Liu, Xingzhong,Gao, Li.

[13]Monitoring Thermal Pollution in Rivers Downstream of Dams with Landsat ETM plus Thermal Infrared Images. Ling, Feng,Ban, Xuan,Li, Xiaodong,Zhang, Yihang,Du, Yun,Foody, Giles M.,Du, Hao. 2017

[14]Effects of River Hydrological Status on Gonadal Development of Breeding Population of Chinese Sturgeon in the Yangtze River: Inferred from a On-site Observation. Zhang, Hui,Wei, Qiwei,Du, Hao. 2013

[15]The immune response of turbot, Scophthalmus maximus (L.), skin to high water temperature. Huang, Z-H,Ma, A-J,Wang, X-A. 2011

[16]Effect of water temperature on digestive enzyme activity and gut mass in sea cucumber Apostichopus japonicus (Selenka), with special reference to aestivation. Gao Fei,Yang Hongsheng,Xu Qiang,Wang Fangyu,Liu Guangbin,Gao Fei. 2009

[17]Variation in the distribution of wintering anchovy Engraulis japonicus and its relationship with water temperature in the central and southern Yellow Sea. Niu Mingxiang,Wang Jun,Wang Jun,Niu Mingxiang,Wang Jun,Niu Mingxiang,Wang Jun. 2017

[18]SEASONAL VARIATIONS OF WATER TEMPERATURE, FOOD AVAILABILITY, SIZE, AND REPRODUCTION ON THE HEMOCYTE PARAMETERS IN THE SCALLOP CHLAMYS FARRERI. Lin, Tingting,Li, Ziniu,Xing, Jing,Lin, Tingting,Zhou, Kai,Lai, Qifang,Yao, Zongli,Li, Ziniu. 2012

[19]TOLERANCE OF FENNEROPENAEUS CHINENSIS "HUANGHAI NO. 2" TO WHITE SPOT SYNDROME VIRUS. Meng, Xian-Hong,Wang, Qing-Yin,Kong, Jie,Luo, Kun,Luan, Sheng,Zhang, Qing-Wen,Liu, Ning,Cao, Baoxiang,Meng, Xian-Hong.

[20]Dietary glutamine supplementation improves growth performance and intestinal digestion/absorption ability in young hybrid sturgeon (Acipenser schrenckii female x Huso dauricus male). Qiyou, X.,Qing, Z.,Hong, X.,Chang'an, W.,Dajiang, S.,Qing, Z..

作者其他论文 更多>>

微信小程序