Molecular characterization of Pod1 during sex development in Chinese tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Wang, Linna

作者： Wang, Linna;Zhu, Ying;Xu, Wenteng;Shao, Changwei;Dong, Zhongdian;Li, Hailong;Cui, Zhongkai;Meng, Liang;Guo, Hua;Tian, Yongsheng;Chen, Songlin;Wang, Linna;Xu, Wenteng;Shao, Changwei;Tian, Yongsheng;Chen, Songlin

作者机构：

关键词： Cynoglossus semilaevis;Expression profile;In situ hybridization;Methylation patterns;Pod1;Sex development

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN： 0006-291X

年卷期： 2017 年 494 卷 3-4 期

页码：

收录情况： SCI

摘要： Pod1 encodes a Class II bHLH transcription factor involved in the development of a number of tissues such as gonad, spleen, lungs and heart. However, to date, little is known about its function in teleosts. In this study, we cloned and characterized Pod1 gene from Cynoglossus semilaevis. This gene contains three exons and two introns, with the full-length cDNA of 918 nucleotides that encodes a 183 amino acid protein with a conserved bHLH domain. Realtime quantitative PCR revealed that Pod1 was predominantly expressed in the testes of C semilaevis. In different stages of testes development, Pod1 expression was undetectable up to 120 days after hatching (dah), and then increased at 210 dah and 1 year after hatching (yah). Furthermore, in situ hybridization (ISH) analysis revealed that Pod1 was mainly localized in the germ cells of testes, but was not detected in ovarian cells; which suggested its possible functions in spermatogenesis of C semilaevis. The methylation profile analysis of Pod1 genomic sequence in the gonads showed that the differences in their putative promoter regions of Pod1 among ovary, male and pseudo-male testes were not obvious. Thus, further research might be needed to evaluate whether Pod1 expression is regulated by epigenetic level. (C) 2017 Published by Elsevier Inc.

分类号：

  • 相关文献

[1]Characterization and expression pattern of r-spondin1 in Cynoglossus semilaevis. Li, Hailong,Xu, Wenteng,Zhu, Ying,Zhang, Ning,Ma, Jialu,Sun, Ai,Cui, Zhongkai,Gao, Fengtao,Wang, Na,Shao, Changwei,Dong, Zhongdian,Li, Yangzhen,Li, Hailong,Xu, Wenteng,Zhu, Ying,Zhang, Ning,Ma, Jialu,Cui, Zhongkai,Gao, Fengtao,Wang, Na,Shao, Changwei,Dong, Zhongdian,Li, Yangzhen,Sun, Ai. 2017

[2]Complement factor I from flatfish half-smooth tongue (Cynoglossus semilaevis) exhibited anti-microbial activities. Xiang, Jinsong,Li, Xihong,Chen, Yadong,Lu, Yang,Yu, Mengjun,Chen, Xuejie,Zhang, Wenting,Zeng, Yan,Sun, Luming,Chen, Song Lin,Sha, Zhenxia,Xiang, Jinsong,Chen, Xuejie,Xiang, Jinsong,Chen, Yadong,Lu, Yang,Yu, Mengjun,Chen, Xuejie,Sha, Zhenxia,Li, Xihong,Chen, Song Lin,Yu, Mengjun,Zhang, Wenting,Zeng, Yan,Sun, Luming.

[3]sghC1q, a novel C1q family member from half-smooth tongue sole (Cynoglossus semilaevis): Identification, expression and analysis of antibacterial and antiviral activities. Zeng, Yan,Xiang, Jinsong,Lu, Yang,Chen, Yadong,Wang, Tianzi,Gong, Guangye,Wang, Lei,Li, Xihong,Chen, Songlin,Sha, Zhenxia,Zeng, Yan,Chen, Yadong,Xiang, Jinsong,Lu, Yang,Gong, Guangye,Wang, Tianzi,Wang, Lei.

[4]Expression Profile Analysis of miR-221 and miR-222 in Different Tissues and Head Kidney Cells of Cynoglossus semilaevis, Following Pathogen Infection. Yan, Hui,Chen, Yadong,Gong, Guangye,Chen, Xuejie,Wang, Tianzi,Chen, Songlin,Sha, Zhenxia,Chen, Yadong,Chen, Songlin,Sha, Zhenxia,Zhou, Shun,Li, Chao.

[5]Dimorphic expression of sex-related genes in different gonadal development stages of sterlet, Acipenser ruthenus, a primitive fish species. Wang, Wei,Niu, CuiJuan,Wang, Wei,Zhu, Hua,Dong, Ying,Tian, ZhaoHui,Dong, Tian,Hu, HongXia.

[6]Uniqueness of the Gossypium mustelinum Genome Revealed by GISH and 45S rDNA FISH. Qiong Wu,Fang Liu,Shaohui Li,Guoli Song,Chunying Wang,Xiangdi Zhang,Yuhong Wang,David Stelly,Kunbo Wang. 2013

[7]Expression Analysis and Binding Assays in the Chemosensory Protein Gene Family Indicate Multiple Roles in Helicoverpa armigera. Zhao-Qun Li,Shuai Zhang,Jun-Yu Luo,Jing Zhu,Jin-Jie Cui,Shuang-Lin Dong.

[8]Molecular Characterization of a New Wheat-Thinopyrum intermedium Translocation Line with Resistance to Powdery Mildew and Stripe Rust. Zhan, Haixian,Li, Guangrong,Pan, Zhihui,Yang, Zujun,Zhan, Haixian,Zhang, Xiaojun,Hu, Jin,Li, Xin,Qiao, Linyi,Guo, Huijuan,Chang, Zhijian,Jia, Juqing,Chang, Zhijian. 2015

[9]Niemann-Pick C2 Proteins: A New Function for an Old Family. Guo, Mengbuo,Liu, Yang,Pelosi, Paolo,Wang, Guirong,Ban, Liping,Song, Li-Mei. 2018

[10]Improved approaches of in situ hybridization and in situ enzyme histochemistry for gene expression analyses in plants. Liu, QF,Hao, ZR,Shen, DL. 1998

[11]Development and identification of wheat-Ag. pulcherrimum addition line and substitution line with BYDV resistance. Wu, DL,Xin, ZY,Chen, X,Xu, HJ,Ma, YZ,Zhang, ZY. 1999

[12]Hybridization of Aegilops caudata with Triticum durum, Triticum aestivum and the detection of alien chromatins. Kong, XY,Zhou, RH,Dong, YS,Jia, JZ. 1999

[13]Identifying the alien chromosomes in wheat - Leymus multicaulis derivatives using GISH and RFLP techniques. Jia, JZ,Zhou, RH,Li, P,Zhao, ML,Dong, YS. 2002

[14]Characterization of Triticum aestivum Psathyrostachys juncea derivatives by genomic in situ hybridization. Zhou, R,Jia, J,Dong, Y,Schwarzacher, T,Reader, SM,Wu, S,Gale, MD,Miller, TE. 1998

[15]Molecular cytogenetic characterization of a new leaf rolling triticale. Ren, Z. L.,Yang, E. N.,Zhang, J. F.,Zou, Y. C.,Yang, Z. J.. 2011

[16]Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA). Li, Y,Xi, YW,Zhang, ZK,Huang, XQ. 2001

[17]Identification of Plasma Membrane Aquaporin in Guard Cells of Vicia faba and Its Role in Stomatal Movement. Huang, RF,Zhu, MJ,Kang, Y,Chen, J,Wang, XC.

[18]Localization, expression, and secretion pathway of diapause hormone in embryo and larva of the silkworm, Bombyx mori. Sun, JS,Chen, FS,Xu, WH.

[19]Production and identification of wheat - Agropyron cristatum (1.4P) alien translocation lines. Liu, Wei-Hua,Luan, Yang,Wang, Jing-Chang,Wang, Xiao-Guang,Su, Jun-Ji,Zhang, Jin-Peng,Yang, Xin-Ming,Gao, Ai-Nong,Li, Li-Hui.

[20]Establishment of 6VS Telocentric Lines of Haynaldia villosa Resistant to Powdery Mildew Induced by Immature Embryo Culture. Li, H,Chen, X,Xin, ZY,Xu, HJ,Du, LP,Ma, YZ.

作者其他论文 更多>>

微信小程序